Protein Purification Reagents and Kits
IBA LifeSciences StrepMAB-Classic monoclonal antibody with HRP conjugate; 75 µl
StrepMAB-Classic is a highly selective, murine monoclonal antibody for detection of Strep-tagII and Twin-Strep-tag fusion proteins in applications like western blots, ELISA, FACS, immunohistochemistry, and immunocytochemistry. It is available coupled to fluorescent dyes (DY-488, DY-549, DY-649) or to horseradish peroxidase (HRP). StrepMAB-Classic HRP is suitable for chromogenic and chemiluminescent reactions.The absorption/emission maxima of the fluorescent dyes are:• DY-488: 493/518 nm• DY-549: 550-560/575 nm• DY-649: 655/676 nmPlease note: For capturing of Strep-tagII and Twin-Strep-tag fusion proteins on solid phases, the use of StrepMAB-Immo or Strep-TactinXT is recommended due to the binding affinity in the pM range.
Vector Laboratories Glycoprotein Eluting Solution for GlcNAc or Chitin-Binding Lectins 100 ml
This Glycoprotein Eluting Solution can be used to elute glycoproteins from agarose-bound N-acetylglucosamine- or chitin-binding lectins. The neutral pH of this solution maximizes the yield of eluted glycoproteins and preserves the activity of the agarose-bound lectins for repeated use. All components of this ready-to-use solution can be subsequently removed by dialysis. This Glycoprotein Eluting Solution offers convenience and superior recovery over standard sugar solutions.
STEMCELL Technologies EasySep™ Human Gamma/Delta T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Gamma/Delta T Cell Isolation Kit is designed to isolate gamma/delta T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-gamma/delta T cells and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. The desired cells are poured off into a new tube. To obtain a custom kit optimized for culture-expanded PBMCs, contact STEMCELL Technologies’ Technical Support at techsupport@stemcell.com.
STEMCELL Technologies EasySep™ RBC Depletion Reagent - 10mL, For processing: 100 mL whole blood processed
The EasySep™ RBC Depletion Reagent is designed to immunomagnetically deplete red blood cells (RBCs) from fresh human peripheral blood, umbilical cord blood, bone marrow or leukapheresis samples by negative selection. RBCs are targeted with Tetrameric Antibody Complexes recognizing Glycophorin A and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube while desired cells are poured off.This product replaces the EasySep™ Human Glycophorin A Depletion Kit (Catalog #18352) for even faster cell isolations.
ProteinSimple Maurice cIEF pI Marker - 8.40, Lyophilized, 210 uL
Maurice C. reagents and consumables are designed for use on with Maurice and Maurice C. systems, and must be used in conjunction with Compass for iCE version 1.0 software.
Pfanstiehl Inc Maltose Monohydrate. High Purity, Low Endotoxin, Low Metals, USP-NF, JP, ChP (M-167) -100G GMP, Multi-compendial CAS: 6363-53-7
Pfanstiehl Maltose Monohydrate High Purity, Low Endotoxin, Low Metals is a multi-compendial carbohydrate utilized in bioprocessing applications and as an infusion stabilizer. Pfanstiehl Maltose has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Maltose meets parenteral (injectable) excipient grade standards, making it ideal as a bioprocessing feed, or as a stabilizing agent for pharmaceutical infusions. Pfanstiehl Maltose is available as USP-NF (National Formulary) JP (Japanese Pharmacopeia) and ChP (Chinese Pharmacopeia) Compendial, U.S.P. , cGMP , pharmaceutical grade .
Izon Science Usa Ltd qEVoriginal/35nm Gen 2 Column, 35 nm pore size, 5-pack, 500 μL sample loading volume, proprietary agarose resin, enhanced separation performance
qEVoriginal/35nm Gen 2 Column, 35 nm pore size, 5-pack, 500 μL sample loading volume, proprietary agarose resin, enhanced separation performance
STEMCELL Technologies EasySep™ Mouse Neutrophil Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse Neutrophil Enrichment Kit is designed to isolate neutrophils from single-cell suspensions of bone marrow or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-neutrophils. Labeled cells are then recognized by Tetrameric Antibody Complexes that are directed against biotin and dextran. These cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
IBA LifeSciences Strep-TactinXT 4Flow high capacity cartridge; 5 ml
Strep-TactinXT 4Flow cartridges contain a 4% agarose coupled with the streptavidin variant Strep-TactinXT and are applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins with HPLC/FPLC devices like Äkta systems (Cytiva). Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow allows the application of denaturing agents such as urea. In comparison to Strep-TactinXT 4Flow, Strep-TactinXT 4Flow high capacity has a higher density of immobilized Strep-TactinXT, which leads to a 2-fold higher yield of purified protein.
Neuromics, Inc BioLock Biotin Blocking Solution, 50 ml
Cultivation media often contain significant amounts of biotin. This is especially the case for mammalian or insect cell culture media.Thus, if proteins from biotin containing extracts or media are intended to be purified via Strep-Tactin chromatography, biotin must be masked by the addition of avidin prior to the application onto the column. BioLock biotin blocking solution allows this masking of biotin (and biotinylated proteins) in a very fast and convenient way.Activity: >70U/mlAdd at least 1U of BioLock solution per µg of biotin.
MONOD BIO INC His Tag NovoBody™ Duo Fc Fusion, 100 μg
The NovoBody™ Duo Fc is a tetravalent anti-His binder with a human Fc fused to the C-terminus, providing higher affinity than conventional antibodies. NovoBody™ Binders are single-chain proteins designed computationally to improve on traditional antibodies. They offer enhanced stability and function while eliminating suboptimal traits like post-translational modifications. Suitable for ELISA, flow cytometry, and capture applications. Tag: Human IgG1 Fc | Conjugation: None | Amount: 100 µg. Learn more at monod.bio
Profoldin Colorimetric DTT Assay Kit
The Colorimetric DTT Assay Kit (Catalog No. DTT100C) measures light absorbance at 590 nm. It is based on the strong reducing property of DTT. The assay is not interfered by phosphate buffer, mono thio compounds such as cysteine or chelators. It is interfered by NADH or NADPH.