Protein Purification Reagents and Kits
Sartorius Biosensor / Ni-NTA (NTA) Tray
Ni-NTA (NTA) Biosensors - One tray of 96 biosensors coated with nickel-charged tris-NTA for both quantitation and kinetic applications of HIS-tagged molecules.
Surmodics Inc StabilGuard Immunoassay Stabilizer, 50mL
StabilGuard™ Immunoassay Stabilizer (BSA-Free) effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. It can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper. The key benefit of the protein free formulation is the elimination of interference and cross reactivity within immunoassay applications.
IBA LifeSciences Strep-TactinXT PE , 50 µl
Strep-TactinXT conjugated to R-Phycoerythrin (PE) is optimized for flow cytometry of cells. It binds to Strep-tagII- or Twin-Strep-tag- fusion proteins with a high affinity.Cells can either be stained directly if they express a strep-tagged surface protein or indirectly via a strep-tagged protein that subsequently binds to the cells (e.g. for sorting antigen-specific B cells). Independent from the chosen method, biotin addition will cause the release of Strep-TactinXT PE from the cell surface. This enables the use of selected cells for further downstream applications.This conjugate is especially recommended for low-affinity or low-expressed targets.
Neuromics, Inc BioLock Biotin Blocking Solution, 50 ml
Cultivation media often contain significant amounts of biotin. This is especially the case for mammalian or insect cell culture media.Thus, if proteins from biotin containing extracts or media are intended to be purified via Strep-Tactin chromatography, biotin must be masked by the addition of avidin prior to the application onto the column. BioLock biotin blocking solution allows this masking of biotin (and biotinylated proteins) in a very fast and convenient way.Activity: >70U/mlAdd at least 1U of BioLock solution per µg of biotin.
Invent Biotechnologies Inc Minute™ Cytoplasmic & Nuclear Extraction Kits for Cells - Spin Column-Based < 15 min (50 Preps)
Minute™ Cytoplasmic and Nuclear Extraction Kit for Cells is composed of optimized cytoplasmic extraction buffer, nuclear protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to quickly separate native cytosol and nuclear proteins from mammalian cells and protoplasts of plant, bacteria, yeast and fungus. Separation of cytoplasmic and nuclear proteins can be accomplished in less than 15 minutes using the protein extraction filter cartridges.
Cell Biolabs Inc QuickTiter™ Adenovirus Titer Immunoassay Kit, 100 assays
Traditional plaque forming unit assays (PFU) used to measure adenoviral functional titer are tedious and can take up to 10 days for results. Cell Biolabs' QuickTiter™ Adenovirus Titer Immunoassay Kit measures functional titer in less time (2.5 days vs. 10 days) with greater accuracy than traditional PFU assays. Each kit provides sufficient reagents for 100 assays.
ProteinSimple Maurice CE-SDS PLUS 1x Sample Buffer, 25 mL
Buffer for samples preparation for CE-SDS PLUS application, 25 mL. For use with CE-SDS PLUS cartridge (PS-MC02-SP), or CE-SDS cartridge (PS-MC02-S) on Maurice (090-000) and Maurice S. (090-001) systems.
STEMCELL Technologies EasySep™ Human B Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human B Cell Isolation Kit is designed to isolate B cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched, desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. This product can be used in place of the EasySep™ Human B Cell Enrichment Kit (Catalog #19054) for even faster cell isolations.
Sigma Aldrich Fine Chemicals Biosciences Nuclei Isolation Kit: Nuclei EZ Prep
The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types. Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.
STEMCELL Technologies EasySep™ Human Gamma/Delta T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Gamma/Delta T Cell Isolation Kit is designed to isolate gamma/delta T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-gamma/delta T cells and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. The desired cells are poured off into a new tube. To obtain a custom kit optimized for culture-expanded PBMCs, contact STEMCELL Technologies’ Technical Support at techsupport@stemcell.com.
Cell Biolabs Inc ViraBind™ Adenovirus Miniprep Kit, 10 preps
Cell Biolabs' ViraBind™ Adenovirus Miniprep Kit provides fast purification of adenovirus supernatant with high yields. The kit uses a special spin column to purify supernatant from a single T75 flask or 10cm dish per prep in about 30 minutes. Total adenovirus recovery is >95%. The kit is suitable for 10 preps.
CUBE BIOTECH 50 ml 100 Ni-INDIGO Agarose for His-tag-purification
50 ml 100 Ni-INDIGO Agarose for His-tag-purification, 50% slurry