Spin Columns and Gravity-flow Columns
IBA LifeSciences Strep-Tactin TACS Agarose Column; 1 ml
Strep-Tactin TACS Agarose columns contain agarose macrobeads coated with the streptavidin variant Strep-Tactin. A special crosslinking permits the use for cell isolation and depletion approaches. They were developed for column-based Traceless Affinty Cell Selection (Fab-TACS/Nano-TACS). In this approach, Fab fragments fused to a Twin-Strep-tag (Fab-Streps) or nanobodies (Nano-Streps) are bound to Strep-Tactin on the agarose matrix. After specifically capturing cells, Fab-/Nano-Streps are released from the Strep-Tactin Agarose by biotin addition. This way the cells are eluted ready for further downstream applications. The same principle is applicable for exosome isolation (Fab-TACS for exosomes).Alternatively to Fab-Streps/Nano-Streps, it is possible to load columns with Twin-Strep-tagged, desthiobiotinylated or biotinylated antibodies (irreversible binding). This makes Strep-Tactin TACS Agarose columns also suitable for negative cell selection or depletion approaches.
IBA LifeSciences Strep-TactinXT 4Flow high capacity column; 1 ml
Strep-TactinXT 4Flow high capacity columns contain a 4% agarose coupled with the streptavidin variant Strep-TactinXT and are applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins via gravity flow. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow high capacity allows the application of denaturing agents such as urea. In comparison to Strep-TactinXT 4Flow, Strep-TactinXT 4Flow high capacity has a higher density of immobilized Strep-TactinXT, which leads to a 2-fold higher yield of purified protein.
IBA LifeSciences Strep-TactinXT 4Flow high capacity column; 5 ml
Strep-TactinXT 4Flow high capacity columns contain a 4% agarose coupled with the streptavidin variant Strep-TactinXT and are applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins via gravity flow. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow high capacity allows the application of denaturing agents such as urea. In comparison to Strep-TactinXT 4Flow, Strep-TactinXT 4Flow high capacity has a higher density of immobilized Strep-TactinXT, which leads to a 2-fold higher yield of purified protein.
IBA LifeSciences Strep-TactinXT 4Flow column; 1 ml
Strep-TactinXT 4Flow columns contain a 4% agarose coupled with the streptavidin variant Strep-TactinXT and are applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins via gravity flow. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose allows for the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow enables the application of denaturing agents such as urea. To reduce the waiting period during the sample and buffer application, the use of the application aid WET FRED is recommended.
Zymo Research Corporation Zymo-Spin™ IIC-XL (50 Pack)
The Zymo-Spin IIC-XL features durable polypropylene construction and contains a unique silica-based matrix that allows purification of up to 25 g of DNA, and 50 g of RNA, in 35 l eluate. Capacity is 900 l.
Bio Rad Laboratories BIO-SPIN 6 COLUMNS
6 Columns; Bio-Rad; Bio-Spin; Includes 100 columns in Tris buffer, 200 collection tubes
Bio Rad Laboratories FREEZE N SQUEEZE DNA GEL EXTR
Column, Spin; Bio-Rad; Freeze N Squeeze; DNA gel extraction; 25
Zymo Research Corporation Zymo-Spin™ I Columns (50 Pack) (Uncapped)
The Zymo-Spin I features durable polypropylene construction and contains a unique silica-based matrix that allows purification of up to 5 g DNA or RNA in 6 l eluate. Capacity is 800 l.
Zymo Research Corporation Zymo-Spin™ IIC Columns (250 Pack) (Capped)
The Zymo-Spin IIC features durable polypropylene construction with an attached cap and contains a unique silica-based matrix that allows purification of up to 25 g of DNA or 50 g of RNA, in 25 l eluate. Capacity is 900 l.
Zymo Research Corporation Zymo-Spin IIN Columns, 50 pack
The Zymo-Spin IIN features durable polypropylene construction and contains a unique silica-based matrix that allows purification of up to 25 µg of DNA, and 50 µg of RNA, in ≥ 25 µl eluate. Capacity is 900 µl.
Epoch Life Science Inc EconoSpin® prepacked G50 Spin Columns with inert support for Dye Terminator Removal, Desalting, Pre-filtration etc. With screw cap and snap off end-tail (50 columns)
PP housing with prepacked G50 on an inert support. 700 ul bed volume. 50-200 ul sample volume. Liquid tight screw cap enables long term buffer storage and eliminates splash when opening/closing. Ideal for protein desalting or post-sequencingg cleanup for Sanger sequencingg. Ideal for small volume batch mode His-tag protein purification when used with Ni+ agarose. 50 columns.
Epoch Life Science Inc 2 ml Collection Tubes for spin columns (1000/PK)
2 ml Collection Tubes for spin columns (1000/PK)
Proteintech Group Inc Spin columns
Spin column protocol for Immunoprecipitation and Glycine elution of proteins from Nano-Trap agarose beads
Proteintech Group Inc Spin columns
Spin column protocol for Immunoprecipitation and Glycine elution of proteins from Nano-Trap agarose beads