Clinical Chemistry Reagents
Biochain Institute Inc Enzyme Ethanol Assay Kit, 100 Assays/PK
BioChain's ethanol assay kit is a simple, direct and automation-ready assay based on alcohol dehydrogenase catalyzed oxidation of ethanol, in which the formed NADH is coupled to the formazan (MTT) chromogen. The intensity of the product color, measured at 565 nm, is proportionate to the ethanol concentration in the sample.
Enzo Life Sciences Matrix metalloproteinase-1 (MMP-1) fluorometric drug discovery kit, RED (1x96wells)
The MMP-1 Fluorometric (also known as fluorimetric) Drug Discovery Kit, RED is a complete assay system designed to screen MMP-1 inhibitors using a quenched fluorogenic substrate OMNIMMP RED: TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab(6-TAMRA)-Ala-Lys-NH2 [TQ3=quencher; GABA=4-aminobutyric acid; Cha=L-cyclohexylalanine; Abu=2-aminobutyric acid; Smc=S-methyl-L-cysteine; Dab=2,4-diaminobutyric acid; 6-TAMRA=6-tetramethylrhodamine]. TAMRA fluorescence is thoroughly quenched by the TQ3 group until cleavage by MMPs separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-1, a potential therapeutic target. The compound NNGH is also included as a prototypic control inhibitor. Alternative name: Interstitial collagenase, Fibroblast collagenase. Applications: Fluorescent detection, HTS Activity assay.
Cell Biolabs Inc Lactate Assay Kit (Colorimetric), 100 assays
Lactic Acid is an alpha hydroxyl acid that can ionize a carboxyl proton to yield the lactate ion, the latter of which exists as two optical isomers L-Lactate and D-Lactate. In the brain lactate, like glucose, is thought to be one of the main sources of energy. High levels of lactate have been found in the extracellular fluid surrounding neurons due to the high metabolic activity of glial cells. Cell Biolabs’ Lactate Assay Kit (Colorimetric) is a simple assay that measures the amount of total lactate present in biological samples in a 96-well microtiter plate format. Sample lactate concentrations are determined by comparison with a known lactate standard. The kit has a detection sensitivity limit of 1.5 uM L-Lactate. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, lactate standards and unknown samples.
VAZYME INTERNATIONAL LLC ADP-Lite Kinase Assay 400 Tests
ADP-Lite Kinase Assay is a universal kinase activity assay kit designed for measuring the activity of any ADP-generating enzyme (e.g., kinases and ATPases). To accurately determine target kinase activity, the assay quantifies the ADP produced during a kinase reaction, which is positively correlated with the kinase activity. This simple, rapid, and homogeneous assay is based on a highly sensitive in vitro bioluminescence technology that requires no excitation light or radioactive materials. It is compatible with a wide range of kinase substrates, including lipids, peptides, proteins, and carbohydrates.The kit contains two core components: ADP-Lite Reagent (for ATP depletion) and Kinase Detection Reagent (for ADP detection). Kinase Detection Reagent is prepared by mixing Kinase Detection Buffer and Kinase Detection Substrate (Lyophilized).
Bioassay Systems QuantiChrom™ Zinc Assay Kit. For quantitative determination of zinc ion Zn2+ and evaluation of drug effects on zinc metabolism.
For quantitative determination of zinc ion Zn2+ and evaluation of drug effects on zinc metabolism. Key Features: Sensitive and accurate. Uses 50 µL samples. Linear detection range 0.12 µM (0.78 µg/dL) to 10 µM (65 µg/dL) zinc in 96-well assay format. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay formats possible. Low interference in biological samples. No pretreatments are needed. Method: OD425nm. Samples: Serum, plasma, urine, saliva, food, beverage and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.78 µg/dL (0.12 µM).
Teco Diagnostics TRIGLYCERIDE GPO-TRINDER
For the quantitative determination of triglycerides in serum or plasma. 10x15mL Vials of Trygliceride-GPO, 1x5mL Vial of Trygliceride-GPO Standard
Cell Biolabs Inc Lipid Doplet Isolation Kit, 50 preps
Lipid droplets are organelles that are rich in lipids, contain a lipid rich core, and are surrounded by a phospholipid monolayer and associated proteins. They are found in adipose tissue of animals and regulate the hydrolysis and storage of neutral lipids; they also serve as storage for cholesterol and acyl-glycerols used to form cell membranes. More recently they have been associated with inflammatory responses, obesity, atherosclerosis, and cancer. Cell Biolabs’ Lipid Droplet Isolation Kit isolates lipid droplets by simple gradient centrifugation, eliminating the need for ultracentrifugation. A lipid droplet source such as tissue or cultured cells is homogenized. A gradient is then created with the homogenate, and the material is centrifuged. The lipid droplets float to the top of the gradient and are recovered by carefully pipetting from the top of the gradient. Each kit provides sufficient reagents to isolate up to 50 preps based on a 50-100 mg tissue or cell sample size.
Biochain Institute Inc Ethanol Assay Kit, 500 Assays/PK
BioChain's ethanol assay kit is a simple, direct and automation-ready assay based on an improved dichromate method, in which dichromate is reduced by ethanol to a bluish chromic (Cr3+) product. The intensity of color, measured at 580 nm, is a direct measure of the alcohol concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits high sensitivity.
Roche Diagnostics COBAS C(501) ISE DILUENT GEN 2
reag, for Roche analyzer, Cobas C501, ISE Diluent gen 2
Bioassay Systems EnzyChrom™ D-Mannitol Assay Kit. For quantitative determination of D-mannitol in biological, food, beverage and agriculture samples.
For quantitative determination of D-mannitol in biological, food, beverage and agriculture samples. Key Features: Fast and sensitive. Use of 20 µL sample. Linear detection range 0.007 to 3 mM mannitol in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Biological, food, beverage and agricultural products. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 7 µM.
Nova Biomedical Corporation NOVA CDV Sample Cup, 100 µL Capacity, Compatible with BioProfile FAST CDV, 500 Cups
The NOVA BIOMEDICAL CORPORATION FAST CDV Sample Cup is designed for accurate cell density and viability analysis, compatible with the Nova Biomedical BioProfile FAST CDV analyzer. With a 100 µL capacity, it prevents cell adherence and enhances mixing, ensuring reliable results for high-density cultures. The proprietary design optimizes sample integrity and allows waste containment for up to 500 cups, setting it apart from standard sample cups.
Dojindo Molecular Technologies Inc Lipid Droplet Assay Kit-Blue allows selective and easy measuring of lipid droplets, applicable for plate reader and flow cytometry (Detection Ex: 376-386 nm/Em: 435-455 nm) kit
Oil Red O and Nile Red staining are widely used to detect lipid droplets. The method by using Oil Red O has the following disadvantages: time-consuming and complicated procedure tends to precipitate in solution, precipitated Oil Red O may cause errors of measurement, and only applicable for fixed cells. Nile Red is not a well-suited method for lipid droplet assay due to its high background. Unlike Oil Red O and Nile Red, the Lipid Droplet Assay Kit - Blue enables selective and easy measuring of lipid droplets as per the protocol. The Staining Dye in this kit minimizes background due to more selective staining ability to LDs than Nile Red. Additionally, the Loading Buffer solution contained in this kit maintains the health of the cells during assays. This kit can be used for live and fixed cells in flow cytometry analysis and plate reader assay. The Lipid Droplet Assay Kit offers two different color options (blue and deep red).
Aat Bioquest Amplite™ Colorimetric Ammonia Quantitation Kit *Blue Color*
Ammonia is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. It is produced in liver and converted to urea through the urea cycle.
Bioassay Systems QuantiChrom™ Hemoglobin Assay Kit. For quantitative determination of hemoglobin and evaluation of drug effects on hemoglobin metabolism.
For quantitative determination of hemoglobin and evaluation of drug effects on hemoglobin metabolism. Key Features: Sensitive and accurate. Linear detection range 0.9 - 200 mg /dL hemoglobin in 96-well plate assay. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Safety. Reagents do not contain any toxic components, e.g. hexacyanoferrate(III) and potassium cyanide used in Drabkin's reagent. Versatility. Assays can be executed in 96-well plate or cuvet. Method: OD400nm. Samples: Blood, serum, plasma, urine etc. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.9 mg/dL.