Restriction Enzymes
New England Biolabs, Inc. BtsI-v2 – 2500 units
BtsI-v2 is an engineered and improved version of BtsI that recognizes the sequence GCAGTGNN^.
New England Biolabs, Inc. EagI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence C^GGCC_G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
INTACT GENOMICS INC PEmax Enzyme 400 µg
Intact Genomics (IG) PEmax enzyme is the purified recombinant Streptococcus pyogenes Cas9 nickase mutant (H840A)-Murine Moloney Leukemia Virus-Reverse Transcriptase (MMLV-RT) fusion protein3-4 containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR prime-editing3-4. The physical purity of this enzyme is ≥85% as assessed by SDS-PAGE with Coomassie blue staining.Product Includes: PEmax enzyme 5x PEmax Nicking Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM MgCl2, 0.1 mM EDTA, pH 6.5 @ 25 °CStore all components at -20°C.
New England Biolabs, Inc. CspCI – 500 units
A restriction endonuclease that recognizes the sequence _NN^NNNNNNNNNNNCAANNNNNGTGGNNNNNNNNNN_NN^.
New England Biolabs, Inc. T7 Endonuclease I – 250 units
T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5' to the mismatch. The protein is the product of T7 gene 3.
New England Biolabs, Inc. EnGen Cas9 NLS, S. pyogenes – 400 pmol
EnGen Spy Cas9 NLS, is an RNA-guided endonuclease that catalyzes site- specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) ). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. EnGen Spy Cas9 NLS contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein.
New England Biolabs, Inc. MluI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence A^CGCGT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BtsI-v2 – 500 units
BtsI-v2 is an engineered and improved version of BtsI that recognizes the sequence GCAGTGNN^.
New England Biolabs, Inc. SnaBI – 2500 units
A restriction endonuclease that recognizes the sequence TAC_^GTA.
New England Biolabs, Inc. BglI – 2000 units
A restriction endonuclease that recognizes the sequence GCCN_NNN^NGGC.
New England Biolabs, Inc. PstI – 50000 units
A restriction endonuclease that recognizes the sequence C_TGCA^G.
New England Biolabs, Inc. NsiI – 5000 units
A restriction endonuclease that recognizes the sequence A_TGCA^T.