Restriction Enzymes
New England Biolabs, Inc. Nb.BsmI – 1000 units
A restriction endonuclease that recognizes the sequence GAATG_CN.
New England Biolabs, Inc. Nb.BssSI – 1000 units
A restriction endonuclease that recognizes the sequence CACGAG.
New England Biolabs, Inc. BsrFI-v2 – 1000 units
A restriction endonuclease that recognizes the sequence R^CCGG_Y.
New England Biolabs, Inc. Esp3I – 1500 units
A Type IIS restriction endonuclease that recognizes the sequence CGTCTCN^NNNN_....
New England Biolabs, Inc. BclI-HF – 15000 units
A high fidelity restriction endonuclease that recognizes the sequence T^GATCA. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
New England Biolabs, Inc. BsaI-HF®v2 – 1000 units
BsaI-HFv2 is a Type IIS restriction enzyme that has been optimized for Golden Gate Assembly and in protocols requiring DNA cutting by BsaI. Works well for any protocol requiring DNA cutting by BsaI. Recommended enzyme for any purpose requiring digestion at the recognition sequence ...5-GGTCTC(N1)/(N5)-3...
- Engineered for improved performance
- Used in Golden Gate Assembly; maintains high activity levels in T4 DNA Ligase Buffer
- Type IIS REs recognize asymmetric DNA sequences; cleave outside their recognition sequence
- 100% activity in rCutSmart Buffer
- Time-Saver qualified for digestion in 5-15 minutes
New England Biolabs, Inc. BsaI-HF®v2 – 5000 units
BsaI-HFv2 is a Type IIS restriction enzyme that has been optimized for Golden Gate Assembly and in protocols requiring DNA cutting by BsaI. Works well for any protocol requiring DNA cutting by BsaI. Recommended enzyme for any purpose requiring digestion at the recognition sequence ...5-GGTCTC(N1)/(N5)-3...
- Engineered for improved performance
- Used in Golden Gate Assembly; maintains high activity levels in T4 DNA Ligase Buffer
- Type IIS REs recognize asymmetric DNA sequences; cleave outside their recognition sequence
- 100% activity in rCutSmart Buffer
- Time-Saver qualified for digestion in 5-15 minutes
New England Biolabs, Inc. BbsI HF - 1000 units
A high fidelity restriction endonuclease that recognizes the sequence GAAGAC(N)2. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind HF restriction enzymes are fully active under a broader range of conditions minimizing off-target products while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. Nt.CviPII – 40 units
A restriction endonuclease that recognizes the sequence ^CCD. Nt.CviPII is a nicking endonuclease that cleaves only one strand of DNA on a double-stranded DNA substrate. The final product on pUC19 is an array of bands from 25 to 200 bp. CCT is cut less efficiently than CCG and CCA. Some of the CCT sites are not cleaved. (Based on NEB observations.)
Abcam Acetylcholinesterase Inhibitor Screening Kit (Colorimetric).
Acetylcholinesterase Inhibitor Screening Kit (Colorimetric).
The product is subject to the following: Abcam Restricted Use Statement
New England Biolabs, Inc. PaqCI - 200 units
PaqCI is a Type IIS restriction enzyme with a 7 basepair recognition sequence: CACCTGC(4/8). It is sourced from an E. coli strain that carries the modified cloned PaqCI gene from Paucibacter aquatile. PaqCI requires multiple sites for efficient cleavage -- PaqCI Activator is included for optimal cleavage. This enzyme is supplied with NEB's rCutSmart Buffer and is heat inactivated.
