Restriction Enzymes
New England Biolabs, Inc. SalI – 10000 units
A restriction endonuclease that recognizes the sequence G^TCGA_C.
New England Biolabs, Inc. DpnII – 5000 units
A restriction endonuclease that recognizes the sequence ^GATC_.
New England Biolabs, Inc. SfcI – 200 units
A restriction endonuclease that recognizes the sequence C^TRYA_G.
New England Biolabs, Inc. MwoI – 2500 units
A restriction endonuclease that recognizes the sequence GCNN_NNN^NNGC.
New England Biolabs, Inc. HpyCH4IV – 2500 units
A restriction endonuclease that recognizes the sequence A^CG_T.
New England Biolabs, Inc. BsaXI – 500 units
A restriction endonuclease that recognizes the sequence _NNN^NNNNNNNNNACNNNNNCTCCNNNNNNN_NNN^.
New England Biolabs, Inc. SbfI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence CC_TGCA^GG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
Cayman Chemical Glycerol Enzyme MIxture 1ea
Glycerol enzyme mixture has been tested and formulated to work exclusively with Cayman’s Glycerol Colorimetric Assay Kit (Item No. 10010755).
New England Biolabs, Inc. PleI – 1000 units
A restriction endonuclease that recognizes the sequence GAGTCNNNN^N_.
New England Biolabs, Inc. KpnI-HF – 20000 units
A high fidelity restriction endonuclease that recognizes the sequence G_GTAC^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. PaqCI - 200 units
PaqCI is a Type IIS restriction enzyme with a 7 basepair recognition sequence: CACCTGC(4/8). It is sourced from an E. coli strain that carries the modified cloned PaqCI gene from Paucibacter aquatile. PaqCI requires multiple sites for efficient cleavage -- PaqCI Activator is included for optimal cleavage. This enzyme is supplied with NEB's rCutSmart Buffer and is heat inactivated.
New England Biolabs, Inc. BsrGI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence T^GTACA. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BsrGI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence T^GTACA. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.