Restriction Enzymes
New England Biolabs, Inc. BfuAI – 250 units
A restriction endonuclease that recognizes the sequence ACCTGCNNNN^NNNN_.
New England Biolabs, Inc. I-SceI – 2500 units
A restriction endonuclease that recognizes the sequence TAGGG_ATAA^CAGGGTAAT. I-SceI is an intron-encoded endonuclease. The intron encoding I-SceI is present in the mitochondria of Saccharomyces cerevisiae.
New England Biolabs, Inc. HinfI – 25000 units
A restriction endonuclease that recognizes the sequence G^ANT_C.
New England Biolabs, Inc. BsiWI-HF – 1500 units
A high fidelity restriction endonuclease that recognizes the sequence C^GTACG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
Cayman Chemical COX-1humnrecombInt 5kU
Source: Active recombinant human His-tagged COX-1 expressed in insect cells • Amino acids: 24-599 • MW: ~70 kDa
New England Biolabs, Inc. WarmStart Nt.BstNBI-1000 u
WarmStart Nt.BstNBI is a site specific nicking endonuclease cloned fromBacillus Stereothermophilus. Restriction Enzyme Cut Site GAGTC(4/-5). It is formulated with a reversibly-bound aptamer which inhibits its nicking activity at temperatures below 40C. WarmStart Nt.BstNBI cleaves only one strand of DNA on a double-stranded DNA substrate. The nicking endonuclease catalyzes a single strand break 4 bases beyond the 3side of the recognition sequence.
New England Biolabs, Inc. FspI – 2500 units
A restriction endonuclease that recognizes the sequence TGC_^GCA.
New England Biolabs, Inc. AvaII – 10000 units
A restriction endonuclease that recognizes the sequence G^GWC_C.
New England Biolabs, Inc. BstNI – 3000 units
A restriction endonuclease that recognizes the sequence CC^W_GG.
New England Biolabs, Inc. SfcI – 1000 units
A restriction endonuclease that recognizes the sequence C^TRYA_G.
INTACT GENOMICS INC PEmax Enzyme 400 µg
Intact Genomics (IG) PEmax enzyme is the purified recombinant Streptococcus pyogenes Cas9 nickase mutant (H840A)-Murine Moloney Leukemia Virus-Reverse Transcriptase (MMLV-RT) fusion protein3-4 containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR prime-editing3-4. The physical purity of this enzyme is ≥85% as assessed by SDS-PAGE with Coomassie blue staining.Product Includes: PEmax enzyme 5x PEmax Nicking Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM MgCl2, 0.1 mM EDTA, pH 6.5 @ 25 °CStore all components at -20°C.
ABclonal Technology NsiI
5...A/ T G C A T...3 3...T A C G T/ A...5 Isoschizomers: Mph1103I EcoT22I Zsp2I