Substrates
Cayman Chemical AMC Arachidonoyl Amide
A fluorometric FAAH substrate used for enzyme activity measurement; release of the fluorescent AMC (ex/em = 360/465 nm) allows for fast and convenient measurement of FAAH activity
Cayman Chemical ALP Substrate 50mg
ALP Substrate has been tested and formulated to work exclusively with Cayman’s Alkaline Phosphatase Colorimetric Activity Assay Kit (Item No. 701710).
Revvity Health Sciences Inc Tag-lite CLIP-Red Labeling Reagent, 500 nmoles
Tag-lite CLIP-Red Labeling Reagent, 500 nmoles
Medchemexpress LLC Benzyloxycarbonyl-L-leucyl-L-leucyl-L-glutamic acid 7-amido-4-methylcoumarin | 348086-66-8 | MFCD01074989 | 99.5% | 664.75 g·mol⁻¹ | C35H44N4O9 | 50MG
Z-Leu-Leu-Glu-AMC is a fluorogenic peptide substrate for measuring postacidic-like (peptidylglutamyl-peptide hydrolyzing, PGPH) activity of the 20S proteasome. Proteolytic cleavage liberates the fluorescent 7-amino-4-methylcoumarin (AMC) reporter, enabling quantitative proteasome activity assays for research use.
- Fluorogenic substrate that releases 7-amino-4-methylcoumarin (AMC) upon cleavage.
- Designed to measure postacidic-like (PGPH) activity of the 20S proteasome.
- Reported purity 99.5%.
- Chemical formula C35H44N4O9.
- Molecular weight 664.75 g·mol⁻¹.
- White to off-white solid appearance.
- Available in multiple pack sizes including 50 mg.
- Storage: powder stable at -80°C for long-term and -20°C for short-term when sealed and protected from moisture.
Seracare Life Sciences Inc STABLEDAB HRP SUBSTRATE KIT
StableDAB remains active and does not precipitate over a 2 week period at use dilution; at ambient temperature it remains stable for 1 week. At 2-8C it remains stable for 2 weeks. Diaminobenzidine (DAB)is the most commonly used substrate for the histochemical detection of peroxidase. It reacts with HRP to produce an insoluble brown precipitate and can be used in staining tissues and on membranes. The main disadvantage of DAB is that it must be used immediatley to avoid reaction with the enzyme. KPL's StableDAB overcomes this disadvantage and can remain ready-to-use for 2 weeks after preparation. Kit includes StableDAB Peroxidase Substrate and Buffer Concentrate.Smooth muscle actin in skin tissue, using streptavidin peroxidase stained with StableDAB, Contrast BLUE counterstain.
Santa Cruz Biotechnology 4-Methylumbelliferyl alpha-L-iduronide (free acid) | 66966-09-4 | MFCD00135894 | 2 mg
4-Methylumbelliferyl alpha-L-iduronide (free acid) | Mol Wt: 352.29 | 66966-09-4 | MFCD00135894 | 2 mg
Medchemexpress LLC Z-leu-leu-glu-AMC | 348086-66-8 | MFCD01074989 | 99.5% | 664.75 | C35H44N4O9 | 10MG
Z-Leu-Leu-Glu-AMC is a fluorogenic tripeptide substrate used to quantify the post-acidic (peptidylglutamyl) hydrolyzing activity of the 20S proteasome. Proteolytic cleavage releases the 7-amino-4-methylcoumarin (AMC) fluorophore, enabling sensitive fluorescence-based assays for biochemical research and enzyme activity screening.
- Fluorogenic substrate that releases AMC upon proteolysis.
- Suitable for measuring peptidylglutamyl-peptide hydrolyzing (PGPH/caspase-like) proteasome activity.
- High reported purity for reliable assay results.
- Available as lyophilized solid and as solution in DMSO for easy reconstitution.
- Characterized by molecular weight 664.75 and formula C35H44N4O9.
Bioworld TMB Stop Reagent, 450 nm, 100 mL
TMB Stop ReagentStop reagent is 0.16M sulfuric acid used to stop the ELISA substrate 3,3’,5,5’ - tetramethylbenzidine (TMB) reaction. Horseradish peroxidase (HRP) enzyme conjugates react withTMB and peroxide to produce a blue byproduct having maximum absorbance at 605nm. Adding Stop reagent changes it to yellow color with absorption maximum at 450 nm and stabilizes the color development.Quantity: 100ml
Bachem H-Ala-AMC
H-Ala-AMC, 77471-41-1, C13H14N2O3, Mr 246.27, 50mg. H-Ala-MCA (free base). Special fluorogenic substrate for aminopeptidase M (microsomal alanyl aminopeptidase, membrane aminopeptidase, APN). Furthermore, the major aminopeptidase from human skeletal muscle showed highest activity with this substrate.
Cayman Chemical G6PDH Substrate 1ea
A PARP1 and PARP2 inhibitor (IC50s 50s 1-14.1 nM) inhibits the proliferation of Capan-1 cells (IC50 0.1 nM) as well as talazoparib- or olaparib-resistant Capan-1 cells (IC50s 1.13 and 0.89 nM respectively) inhibits the proliferation of BRCA1 mutant HCC1937 breast cancer cells (IC50 4.54 nM) reduces tumor growth in an olaparib-resistant MDA-MB-436 breast cancer mouse xenograft model at 12.5 and 25 mg/kg
Cayman Chemical Ascorbate Substrate 1ea
Morphine ELISA Standard has been tested and formulated to work exclusively with Cayman’s Morphine ELISA Kit (Item No. 501940).
Enzo Life Sciences Ac-IEPD-pNA (5mg)
Colorimetric granzyme B substrate. Combinatorial and kinetic studies have found the IEPD sequence to be optimal for cleavage of a tetrapeptide substrate by recombinant granzyme B, with a Km for Ac-IEPD-pNA of 57 µM. Cleavage of the substrate by granzyme B releases pNA, which can be detected by its absorbance at 405 nm. Alternative name: Caspase 8 substrate (chromogenic), Granzyme B substrate (chromogenic). Purity: ≥95%. Solubility: Soluble to at least 100mM in DMSO. Long Term Storage: -20°C.