Substrates
Bioworld BCIP, 1 g
BCIP Also known as 5-Bromo-4-Chloro-3-Indolyl Phosphate. A widely used chromogenic substrate for alkaline phosphatase in enzyme-linked assay systems. BCIP may be used alone, or may act in a coupled reaction with NBT. Upon cleavage of the phosphate group from the molecule, BCIP forms a bright blue, insoluble product. This product then reacts to form a chromogen homodimer. This reaction may be used to amplify the signal by catalyzing the reduction of a tetrazolium salt (usually NBT) to form an intenselycolored, insoluble formazan chromogen. Technical Information:Ultra Pure GradePurity (enzymatic): >99.0%Purity (UV): >99.0%Inhibitors (enzymatic): <0.1%Solubility (0.1% phosphate buffer): Clear and haze-freeSolubility (0.10% DMF): Pass (Clear/Haze-free)Melting Point: 195-197CA260 (1mg/ml, DMF): 0.010Ref: Unger, et al., Am. J. Surg. Pathol., 10(1): 1-8, 1986.
Bioworld IPTG (Isopropyl-B-D-ThioGalactopyranoside) Solution, 100mM, 1 mL
Isopropyl-β-D-Thiogalactopyranoside, an inducer of β-galactosidase is used to promote the expression of proteins in cells controlled by the lac operator system. Supplied as 1ml or 10x1ml of 100mM ready-to-use solution to be used with X-gal for blue white colony screening. Techncial Information:Biotechnology Grade.Ready-to-use solution Sterile filtered (0.22μM)Dioxane freeApplication: Petriplates: Use 40 μl per Petriplates (9 cm diameter). LB agar: Use 1μl/mL of liquid LB agar medium just before pouring (~50°C).Liquid culture: Use 1μl/mL of liquid culture medium.Sold as 1mL or 10 x 1mL.
Seracare Life Sciences Inc DAB-C SUBSTRATE CONCENTRATE
Available as a substrate component of the HistoMark Orange immunohistochemistry substrate kit or as a stand-alone product. Requires Enhance Orange Buffer and Peroxide Solution for use.
Medchemexpress LLC Boc-Gly-Lys-Arg-AMC | 109358-48-7 | 616.71 | 25 MG
Boc-Gly-Lys-Arg-AMC is a polypeptide identified through peptide screening, a research tool that primarily uses immunoassay to pool active peptides. This method is applicable for protein interaction, functional analysis, and epitope screening, particularly in agent research and development.
- Identified through peptide screening
- Utilizes immunoassay to pool active peptides
- Applicable for protein interaction studies
- Suitable for functional analysis
- Useful for epitope screening
- Valuable in agent research and development
- For research use only
Medchemexpress LLC Ac-YVAD-AMC | 149231-65-2 | MFCD00171369 | 99.6% | 665.69 | 10 MG
Ac-YVAD-AMC is a synthetic tetrapeptide substrate and a caspase inhibitor. It is utilized in research for inhibiting bacteria-induced cell death within hypersensitive response (HR) cells.
- Cleaved by Caspase-1, releasing fluorogenic AMC.
Bachem Boc-Lys(Tfa)-AMC
Boc-Lys(Tfa)-AMC, 97885-44-4, C23H28F3N3O6, Mr 499.49, 250mg. Fluorogenic substrate for assaying histone deacetylase (HDAC) 4, 5 and 7 activity in a protease-coupled assay. Suitable substrate for HDAC 8. HDAC cleavage yields Boc-Lys-AMC.
Medchemexpress LLC Boc-Glu-Lys-Lys-AMC | 73554-85-5 | 99.9% | 660.76 | 10 MG
Boc-Glu-Lys-Lys-AMC is a sensitive fluorogenic substrate for urokinase-activated plasmin. It is intended for research use only.
- Sensitive fluorogenic substrate
- For urokinase-activated plasmin
- Research use only
Selleck Chemical LLC X-GAL
X-GAL (BCIG) is a widely used chromogenic -galactosidase substrate -galactosidase cleaves X-gal and produce an insoluble detectable blue compound X-Gal is applied in blue-white selection of recombinant bacterial colonies with the lac genotype
Bachem DABCYL-LYS-HCOV-SARS REPLIC5MG
DABCYL-Lys-HCoV-SARS Replicase Polyprotein 1ab (3235-3246)-Glu-EDANS trifluoroacetate salt | 730985-86-1 | MFCD08458566 | 5MG
CPC Scientific DABCYL-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-EDANS (trifluoroacetate salt) 1MG
TACE FRET substrate I that is derived from the cleavage sequence of pro-TNFa recognized by the TNFa-cleaving enzyme (TACE). TNFa is presumably shed from cell membranes by TNFa-cleaving enzyme (TACE). Incubation of the TACE substrate with recombinant human TACE gives a specific cleavage to restore the quenched fluorescence. The substrate is widely used to screen inhibitors of TNF-convertase.ONE-LETTER SEQUENCE: Dabcyl-LAQAVRSSSR-EDANSMOLECULAR FORMULA: C70H104N22O18S1MOLECULAR WEIGHT:1573.80STORAGE CONDITIONS: -20 5C, CAS REGISTRY NUMBER: [396716-14-6], SYNONYMS: DABCYL-TNF--EDANS (-4 to +6) (human)RESEARCH AREA: ApoptosisREFERENCES: A.J. H.Gearing et al., Nature , 370, 555 (1994); K.M. Mohler et al., Nature , 370, 218 (1994); Becker BF, et al.Biol Chem 383, 1821-6 (2002); Mohler KM, et al., Nature 370, 218-20. (1994);
Cayman Chemical Ac-QPKK Ac-AMC 1mg
A fluorogenic substrate for SIRT1, SIRT2, and SIRT3; AMC is released upon enzymatic cleavage by SIRT1, SIRT2, or SIRT3 and its fluorescence can be used to quantify SIRT1, SIRT2, and SIRT3 activity; ex/em = 340-360/440-460 nm, respectively
CPC Scientific H-Asp-Arg-Val-Tyr-Ile-His-Pro-Cys-His-Leu-Leu-Tyr-Tyr-Ser-OH (trifluoroacetate salt) 1MG
ONE-LETTER SEQUENCE: DRVYIHPCHLLYYSMOLECULAR FORMULA: C83H119N21O21S1MOLECULAR WEIGHT:1779.1STORAGE CONDITIONS: -20 5CRESEARCH AREA: Cardiovascular