Substrates
Biotium Coelenterazine HCP
Coelenterazine hcp is a synthetic derivative of coelenterazine. Its aequorin complex generates luminescence intensity 190 times higher and has a faster response time to calcium compared to native coelenterazine.Coelenterazines are luminescent enzyme substrates for apoaequorin and Renilla luciferase. Apoaequorin is used as a calcium indicator. It binds coelenterazine to form an aequorin complex that emits light upon calcium binding and measures concentrations ~0.1 uM to >100 uM. Renilla luciferase is widely used as a reporter of transcription regulation and as a donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Coelenterazines are also used for chemiluminescent detection of superoxide anions and peroxynitrite in cells or tissues. Coelentrazine analogs and water-soluble Aquaphile™ Coelenterazines are also available.Properties : Yellow solid soluble in MeOH or EtOH; Store at -20°C, protect from light; C25H25N3O2; MW: 399.49.
Biotium Coelenterazine E
Coelenterazine e is a synthetic derivative of coelenterazine. It is a superior substrate for Renilla luciferase generating nearly 40% more light, as well as has an initial light intensity more than 7 times higher than native coelenterazine. Coelenterazine e/Renilla luciferase has two emission peaks centered at 418 and 475 nm, respectively. Coelenterazine e is also a good substrate for apoaequorin compared with native coelenterazine. The e-aequorin compelx has a faster luminescence rise time and shows two emission peaks at 405 and 465 nm respectively, with the ratio of the peak heights dependent on calcium concentration in the range pCa 5-7. The dual emission makes ratiometric calcium detection possible.Properties : Dark brown solid soluble in MeOH or EtOH; Store at -20°C, protect from light; C28H23N3O3; MW: 449.50.
AURORA BIOLABS LLC TR-FRET PARP2 Trapping Assay Kit, 384 rxn
TR-FRET PARP2 Trapping Assay Kit, 384 rxn
Abcam C12FDG (5-Dodecanoylaminofluorescein di-ß-D-galactopyranoside), 5MG
MW 853.9 Da, Purity >95%. C12FDG (5-Dodecanoylaminofluorescein di-β-D-galactopyranoside). Achieve your results faster with highly validated, pure and trusted compounds.
The product is subject to the following: Abcam Restricted Use Statement
CPC Scientific N-Methylsulfonyl-DPhe-Gly-Arg-pNA (trifluoroacetate salt) 1MG
Determination of t-PA(tissue plasminogen activator) in purified preparation and in cell culture supernatents in the micro gram scale. Working concentration ~0.25 mM.ONE-LETTER SEQUENCE: N-Methylsulfonyl-DPhe-GR-pNAMOLECULAR FORMULA: C24H32N8O7S1MOLECULAR WEIGHT:576.5STORAGE CONDITIONS: -20 5CRESEARCH AREA: Apoptosis
Aniara Diagnostica LLC BIOPHEN CS-01(38) THROMB 12 EA
This item MFG by HYPHEN BioMed and Dis. by Aniara Diagnostics. Chromogenic substrate for thrombin, lyophilized. Peptide sequence H-D-Phe-Pip-Arg-pNa, 2HCl. 12 vials x 25 mg.
Sigma Aldrich Fine Chemicals Biosciences 4-Nitrophenyl beta-D-glucopyranoside >=98% (TLC) | 2492-87-7 | MFCD00006593 | 5G
4-Nitrophenyl beta-D-glucopyranoside >=98% (TLC) | Purity: >=98% (TLC) | Mol Wt: 301.25 | 2492-87-7 | MFCD00006593 | 5G
Sigma Aldrich Fine Chemicals Biosciences Azocasein protease substrate | 102110-74-7 | MFCD00130592 | 1g
Azocasein protease substrate Purity: MW: | 102110-74-7 | MFCD00130592 | 1g
Revvity Health Sciences Inc Tag-lite CLIP-Red Labeling Reagent, 100 nmoles
Tag-lite CLIP-Red Labeling Reagent, 100 nmoles
Medchemexpress LLC Z-Nle-Lys-Arg-AMC acetate | 1135364-14-5 | 99.6% | 706.83 | C36H50N8O7.xC2H4O2 | 10 MG
Z-Nle-Lys-Arg-AMC acetate is a fluorogenic peptide substrate that specifically monitors cathepsin B activity across a broad pH range. Supplied as a solid powder for research use, it produces a fluorescent signal upon enzymatic cleavage, enabling sensitive activity assays and inhibitor screening.
- Monitors cathepsin B activity across a broad pH range.
- Generates fluorescent signal upon enzymatic cleavage for sensitive assays.
- High purity (99.58%) with molecular weight 706.83 for consistent performance.
- White to off-white solid suitable for sealed storage.
- Stable under recommended storage: powder at -80°C (2 years) or -20°C (1 year); in solvent at -80°C (6 months) or -20°C (1 month).
- Supplied in 10 mg quantity for laboratory assays.
Apexbio Technology LLC D-Luciferin sodium salt(Synonyms: Sodium D-Luciferin, Luciferin sodium salt, Luciferase substrate), 50mg, CAS: 103404-75-7.
D-Luciferin sodium salt (CAS 103404-75-7) is a bioluminescent substrate widely utilized in biomedical research and diagnostic applications Upon entering cells D-Luciferin undergoes enzymatic oxidation catalyzed by luciferase enzymes a reaction requiring ATP and molecular oxygen that emits detectable light This ATP-dependent bioluminescent reaction enables non-invasive monitoring of intracellular metabolic status and energy availability Commonly employed in bioluminescence imaging D-Luciferin sodium salt serves as a powerful tool to assess cellular viability metabolism and gene expression in research investigating cell biology oncology and metabolism
Biotium NucView 530 Caspase-3 Substrate, 1 mM in DMSO, Trial Size
NucView Caspase-3 Substrates are novel fluorogenic substrates for real-time or endpoint detection of apoptosis in live cells. The non-toxic, no-wash substrates do not inhibit caspase-3 unlike other caspase substrates or FLICA assays permitting multiday analysis. Consisting of a high-affinity DNA dye linked to a DEVD caspase-3 substrate moiety, the cell permeable non-fluorescent substrate is cleaved by caspase-3 in apoptotic cells to emit fluorescence, allowing detection of both caspase-3 activity and apopototic nuclear morphology. Suitable for flow cytometry, fluorescence microscopy, or live cell imaging systems, staining is compatible with fixation and permeabilization for immunostaining. NucView 488 is validated in over 100 cell types and 200 publications. Available with blue (NucView 405), green (NucView 488), or red (NucView 530) fluorescence, in DMSO or PBS for DMSO-sensitive cells, and as kits with apoptosis or necrosis probes. NucView technology is covered by U.S. patents.
Medchemexpress LLC D-luciferin potassium | 115144-35-9 | MFCD00044928 | 99.97% | 318.41 | C11H7KN2O3S2 | 100MG
D-luciferin potassium is the water-soluble potassium salt of D-luciferin, the natural substrate for firefly luciferase. It is provided as a solid for preparation of aqueous solutions used in both in vitro and in vivo bioluminescent assays and imaging applications.
- Water-soluble potassium salt of D-luciferin.
- Serves as substrate for firefly luciferase in bioluminescent assays.
- High purity suitable for research applications (reported 99.97%).
- Applicable to both in vitro assays and in vivo imaging workflows.
- Soluble in aqueous buffers for easy preparation of working solutions.
- Molecular weight 318.41; chemical formula C11H7KN2O3S2.