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Biotin-binding protein conjugates for biotinylated antibody probe detection in ELISA and western blot protocols; products may include peroxidase and poly-HRP conjugates, high-intensity fluorescent conjugates, and infrared conjugates.
Thermo Scientific™ CaptureSelect™ Biotin Anti-Factor X Conjugate consists of a 13-kDa recombinant single domain anti-Factor X antibody fragment chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the antibody fragment.
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The In Vitro Biotinylation Kit (with BirA Tagless Enzyme) is a kit designed for in vitro addition of biotin to biotin-acceptor peptides using BirA. This kit comes in a convenient, easy-to-use format and contains all the components necessary to label biotin-acceptor peptides with biotin. The BirA protein provided is a tagless recombinant biotin protein ligase (EC6.3.4.15). It efficiently catalyzes the specific attachment of biotin to the AviTag™. It is a robust reaction in a wide range of conditions, such as 20-40 µM of protein substrate (biotin-acceptor peptide) concentrations and can tolerate up to 1M NaCl and 20% glycerol in the reaction.
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Conventional ImmunoGold Reagents are monodisperse size population makes the conjugates suited for multiple labeling with no overlap. The Conventional Immunogold Reagents are the classical conjugates in immuno electron microscopy; they are a good choice when the antigen is abundant and the accessibility of the antigen is relatively good.
The conventional labeling approach in transmission and scanning electron microscopy utilizes secondary immunogold reagents based on particles that can be observed without enhancement. The particle population is monodisperse and thus shows minimal size variation and overlap.
The reagents are supplied in PBS with 1% Bovine Serum Albumin and 15 mM NaN3. Particle size: EM-grade 25nm
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MW 67000 g/mol. Fluorescent conjugate useful for DNA and RNA microarray analyses and in detecting biotin-labeled antibodies. It can be employed with other fluorescein or coumarin conjugates to simultaneously localize two or three antigens in the same tissue section - excitation/emission maxima are well separated from those of fluorescein.