Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Molecular Devices Time Resolved Filter, 642 nm, 10 nm Bandwidth
Manufactured by Molecular Devices, this time-resolved interference filter provides a narrow optical band centered at 642 nm with a 10 nm full width at half maximum (FWHM) for improved signal specificity in time-resolved fluorescence assays. It is intended as a plug-in accessory for compatible multi-mode microplate readers to enable sensitive TRF measurements.
- Center wavelength: 642 nm.
- Bandwidth: 10 nm FWHM.
- Filter type: time-resolved fluorescence interference filter.
- Optimized for time-resolved fluorescence assays to reduce background signal.
- Designed as a plug-in accessory for compatible multi-mode microplate readers.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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The Molecular Devices 610 nm LP luminescence filter (cat. #6590-0117) is a long-pass emission filter optimized for luminescence detection and BRET acceptor measurements. It is intended for use as an acceptor/emission filter in NanoBRET assays and is documented for compatibility with filter-based multi-mode microplate readers such as the SpectraMax iD5.
- Provides a long-pass emission cutoff at 610 nm.
- Optimized for luminescence and BRET acceptor detection.
- Designed for use in filter-based microplate reader optical systems.
- Supports assays requiring acceptor emission separation in NanoBRET workflows.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Molecular Devices Absorbance Filter, 630 nm, 8 nm Bandwidth, FilterMax
Molecular Devices absorbance filter (MPN 6590-0028) centered at 630 nm with an approximately 8 nm full-width bandwidth for use in absorbance-based microplate assays.
- Center wavelength 630 nm for red-region absorbance measurements.
- 8 nm bandwidth for narrowband detection.
- Intended for absorbance-based microplate assays.
- Fits microplate readers that accept replaceable absorbance filters.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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This accessory offers transmission, reflectance, and visible viewing modes all in one device; no realignment or extra equipment required.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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