Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
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Perkin Elmer US LLC Micro ATR Objective for the Multiscope Systems
This accessory offers transmission, reflectance, and visible viewing modes all in one device; no realignment or extra equipment required.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Enterprise Technology Solutions Customizable Index Dividers
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Molecular Devices Fluorescence Polarization Filter, 595 nm, 25 nm, Vertical/Horizontal
Fluorescence polarization filter set with a 595 nm center wavelength and 25 nm bandwidth, supplied as a pair of orthogonally polarized filters for fluorescence polarization assays. Designed for removable-filter multi-mode microplate readers and optimized for TAMRA-labeled substrates.
- Center wavelength: 595 nm
- Bandwidth: 25 nm
- Polarization: vertical and horizontal
- Quantity: set of 2 filters
- Intended application: fluorescence polarization assays with TAMRA-labeled substrates
- Compatible with removable-filter multi-mode microplate readers
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Molecular Devices Fluorescence Polarization Filter, 635 nm, Emission
Fluorescence polarization emission filter centered at 635 nm for use with SpectraMax iD5 microplate readers. The filter is intended for fluorescence polarization assays and mounts in the instrument filter slide for straightforward exchange.
- Provides emission detection centered at 635 nm for red-shifted fluorophores.
- Designed for fluorescence polarization measurements.
- Fits 25 mm filter slides used in SpectraMax iD5 readers.
- Installs into the instrument filter slide for tool-free exchange.
- Reduces background signal for improved polarization read accuracy.
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