Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Total VEGFR2 Detection Kit, 500 Assay Points
HTRF Human Total VEGFR2 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Anti histone H3K4 Me2-EuK mAb conjugate, 10,000 Assay Points
HTRF Anti histone H3K4 Me2-EuK mAb conjugate, 10,000 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Multiplex Human Phospho-CDK1 (Tyr15 & Total) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Multiplex Human Phospho-CDK1 (Tyr15 & Total) Detection Kit, 100 Assay Points
Molecular Devices Validation Plate, Absorbance, NIST-Traceable, ISO 17025 Recertifiable
The SpectraTest ABS2 Validation Plate from Molecular Devices is an automated absorbance validation tool used to verify the optical performance and sensitivity of absorbance-capable microplate readers. It provides NIST-traceable photometric checks and supports recertification to maintain GMP and GLP compliance; requires SoftMax Pro software version 6.5.1 or higher.
- Validates absorbance sensitivity and photometric accuracy.
- Automated certificate value entry for streamlined validation workflows.
- Compatible with absorbance-capable microplate readers and automated handling systems.
- Supports annual recertification with ISO 17025 traceability.
- Designed for GMP and GLP instrument performance verification.
- Factory recertification and repair services reduce downtime.
Molecular Devices Photometric Filter, 405 nm, 10 nm Bandwidth
AD 340 photometric absorbance filter for narrowband absorbance measurements at 405 nm. Designed as a replacement or accessory optical filter for absorbance channels in microplate reader assays, it provides a focused transmission band for reliable endpoint and kinetic measurements.
- Center wavelength: 405 nm.
- Bandwidth: 10 nm.
- Measurement type: Absorbance (photometric).
- Typical applications: Endpoint and kinetic microplate assays.
- Form factor: Optical bandpass photometric filter.
Molecular Devices Absorbance Filter, 480 nm, 8 nm, FilterMax
This replacement absorbance photometric filter is designed for use in Molecular Devices microplate readers and provides narrowband absorbance detection centered at 480 nm for photometric assays that require precise wavelength selection.
- Provides narrowband absorbance detection centered at 480 nm.
- 8 nm full width at half maximum bandwidth for selective measurements.
- Intended for absorbance and photometric microplate assays.
- Compatible with microplate reader filter holders used in multi-mode readers.
- Useful for assays requiring blue-green wavelength detection.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human & Mouse Total PLK1 Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human & Mouse Total PLK1 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total CREBBP Detection Kit, 500 Assay Points
HTRF Human & Mouse Total CREBBP Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF cIAP1 BIR2 Binding Kit, 100,000 Assay Points
HTRF cIAP1 BIR2 Binding Kit, 100,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total BRD7 Detection Kit, 500 Assay Points
HTRF Human & Mouse Total BRD7 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total Rb Detection Kit, Control Lysate
HTRF Human Total Rb Detection Kit, Control Lysate
Molecular Devices Fluorescence Filter, 425 nm, 40 nm, With Holder
Molecular Devices fluorescence filter with holder provides a 425 nm central wavelength and 40 nm bandwidth for selective optical isolation in fluorescence, luminescence, and time-resolved fluorescence (TRF) assays. The filter comes mounted in a holder for integration into compatible multi-mode microplate readers and imaging modules.
- Designed for fluorescence, luminescence, and TRF assays.
- 425 nm central wavelength with 40 nm bandwidth.
- Supplied in a holder for instrument mounting.
- Reduces background signal and improves specificity.
- Suitable for integration with multi-mode microplate readers and imaging systems.
Revvity Health Sciences Inc HTRF Human XIAP / BIR3 Binding Kit, 100,000 Assay Points
HTRF Human XIAP / BIR3 Binding Kit, 100,000 Assay Points
Revvity Health Sciences Inc AlphaLISA Surefire Ultra Human Phospho-NPM (Thr199) Detection Kit, Control Lysate
AlphaLISA Surefire Ultra Human Phospho-NPM (Thr199) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total CDK1 Detection Kit, 10,000 Assay Points
HTRF Human Total CDK1 Detection Kit, 10,000 Assay Points