Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Sigma Aldrich Fine Chemicals Biosciences PyrexR low actinic media s1L
Heavy-duty low actinic amber glass bottles protect light-sensitive materials. Include linerless one-piece autoclavable orange PP plug-seal caps (size GL 45) with drip-free pouring rings. Permanent white enamel graduations and marking spots.
Molecular Devices Multi-Mode Microplate Reader, Five-mode, 6-384 wells, Hybrid optics
The SpectraMax iD5 is a five-mode multi-mode microplate reader designed for routine and advanced laboratory assays, combining monochromator and filter optics with an intuitive touchscreen and SoftMax software to support absorbance, fluorescence, luminescence, TRF, and FP workflows.
- Five detection modes: absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, fluorescence polarization.
- Plate formats: compatible with 6 to 384 well microplates.
- Optics: hybrid monochromator and filter-based reads with xenon flash lamp.
- Detectors: photomultiplier tube and photodiode.
- Temperature control: 5 °C above ambient to 66 °C.
- Shaking modes: linear, orbital, double orbital.
- Software: embedded SoftMax Touch interface and SoftMax Pro compatibility for data analysis.
- Dimensions and weight: 15.79 x 20.94 x 23.54 in (40.1 x 53.2 x 59.8 cm); 88.1 lb (40 kg).
Revvity Health Sciences Inc HTRF cIAP1 BIR2 Binding Kit, 500 Assay Points
HTRF cIAP1 BIR2 Binding Kit, 500 Assay Points
Molecular Devices Fluorescence Filter, 590 nm, 20 nm bandwidth, Holder
Molecular Devices fluorescence emission bandpass filter with holder designed for narrow emission detection at 590 nm. The filter provides a 20 nm full width passband and installs in a compatible filter holder for microplate reader fluorescence assays.
- Provides a narrow emission passband centered at 590 nm.
- 20 nm full width bandwidth for selective signal detection.
- Supplied mounted in a holder for straightforward instrument installation.
- Optimized for fluorescence emission measurements to improve assay specificity.
- Reduces spectral crosstalk for improved signal-to-noise ratio.
Leica Biosystems Leica Microsystems Filter Cube 405 nm (for Leica fluorescence microscopes)
Filter cube for 405 nm fluorescence imaging. Provides the excitation, dichroic, and emission filtering required for capturing fluorescence excited near 405 nm. Intended as a drop-in accessory for compatible microscope filter turrets to enable bright, high-contrast imaging of blue/violet-excited fluorophores in research and clinical workflows.
- Provides 405 nm excitation band for fluorescence imaging.
- Includes matched excitation, dichroic, and emission filter elements.
- Designed for drop-in installation in compatible filter turret assemblies.
- Suitable for imaging DAPI, Hoechst, and similar 405 nm-excited fluorophores.
- Used in fixed and live-sample fluorescence microscopy workflows.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Phospho-c-FOS (Ser32) Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human Phospho-c-FOS (Ser32) Detection Kit, 100 Assay Points
THERMO SCI LSG 3P Robotic Tray including adapter for plate without lid
Robotic Tray including adapter for plate without lid
Revvity Health Sciences Inc HTRF CD64 Cellular Binding Kit, 1,000 Assay Points
HTRF CD64 Cellular Binding Kit, 1,000 Assay Points
Molecular Devices Reader Cable, MAC-Series 1, Emax and Vmax Compatible
Molecular Devices replacement cable designed to connect MAC-Series 1 readers to compatible Emax and Vmax microplate readers for instrument control and data transfer.
- Replacement interconnect cable for legacy microplate readers.
- Enables data and control signal transfer between instruments.
- Plug-and-play design for straightforward installation.
- Supplied as a single unit for spare parts inventory.
Revvity Health Sciences Inc HTRF Human & Mouse Total HDAC5 Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total HDAC5 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human LAG3 / MHCII Binding Kit Standard
HTRF Human LAG3 / MHCII Binding Kit Standard
Molecular Devices Photometric Filter, 540 nm, 8 nm Bandwidth
Molecular Devices AD 340 photometric filter provides a narrow-band absorbance measurement centered at 540 nm for compatible multimode microplate readers. It is intended as a replacement or accessory filter for routine absorbance assays and instrument maintenance.
- Provides a 540 nm center wavelength for absorbance measurements.
- 8 nm full width at half maximum (bandwidth) for narrow-band detection.
- Bandpass design reduces out-of-band signal and improves assay specificity.
- Designed for use with compatible multimode microplate readers; verify instrument compatibility before ordering.
- Packaged as a single replacement filter for instrument maintenance and routine swaps.