Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Enterprise Technology Solutions Customizable Print Dividers
Customizable Print-On Dividers Unpunched 5-Tab 11 x 8 5 White 5 Sets
Molecular Devices Enhanced TRF Module, Time-Resolved Fluorescence, SpectraMax iD5 Compatible
The Enhanced TRF Module expands time-resolved fluorescence performance of SpectraMax iD5 and iD5e microplate readers, enabling TRF, TR-FRET, HTRF, IMAP, and ScanLater western blot reads with improved sensitivity for lanthanide assays.
- Improved sensitivity for Europium detection down to 10 fM.
- Enables TRF, TR-FRET, and HTRF assay read modes.
- Supports ScanLater western blot TRF reads.
- Operates with configurable flash intervals for time-resolved reads.
- Compatible with exchangeable excitation and emission filter slides sold separately.
- Requires compatible data acquisition software for some TR-FRET read modes.
Revvity Health Sciences Inc HTRF Human LAG3 / MHCII Binding Kit Standard
HTRF Human LAG3 / MHCII Binding Kit Standard
Molecular Devices Photometric Filter, 540 nm, 8 nm Bandwidth
Molecular Devices AD 340 photometric filter provides a narrow-band absorbance measurement centered at 540 nm for compatible multimode microplate readers. It is intended as a replacement or accessory filter for routine absorbance assays and instrument maintenance.
- Provides a 540 nm center wavelength for absorbance measurements.
- 8 nm full width at half maximum (bandwidth) for narrow-band detection.
- Bandpass design reduces out-of-band signal and improves assay specificity.
- Designed for use with compatible multimode microplate readers; verify instrument compatibility before ordering.
- Packaged as a single replacement filter for instrument maintenance and routine swaps.
Revvity Health Sciences Inc HTRF Human & Mouse LC3B II Detection Kit, Control Lysate
HTRF Human & Mouse LC3B II Detection Kit, Control Lysate
Enterprise Technology Solutions Customizable TOC Ready Index M
Customizable TOC Ready Index Multicolor Tab Dividers Uncollated 8-Tab 1 to 8 11 x 8.5 White 24 Sets
Revvity Health Sciences Inc HTRF AAV3B Detection Kit, 500 Assay Points
HTRF AAV3B Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human CCL22 Detection Kit Standard
HTRF Human CCL22 Detection Kit Standard
Revvity Health Sciences Inc HTRF Human Androgen Receptor Variant 7 Detection Kit, 500 Assay Points
HTRF Human Androgen Receptor Variant 7 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human XIAP / BIR3 Binding Kit Standard
HTRF Human XIAP / BIR3 Binding Kit Standard
Alkali Scientific ASI™ 6-Well Confocal Plate (35m), Clear, Adhesion Type, Sterile, 4/Cs, PS/Glass, Non-Treated (Non-Charged/ Hydrophobic), 100/Cs, 22 x 22 x 0.13mm, Hole 3.14mm
ASI™ confocal products allow researchers to acquire high resolution microscopic images of cells in 35 mm culture dishes or 6 well plates. Low auto-fluorescence glass and FLux were used, ideal for uses of these products are confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Surface materials for confocal products: Glass: naturally charged, good growth surface for adherent cellsFLux: surface modified plastic film (188 μm thick), optimal for confocal microscopy ▪ Bottom materials: Glass/FLux (no surface treatment for glass bottom)▪ Dish color: Clear▪ Dish size: 35 Ø▪ Plate size: 6well Plate▪ Hole size: 13 Ø / 20 Ø / 25Ø▪ Non – pyrogenic▪ Non – cytotoxic▪ DNase / RNase – free▪ Human DNA – free
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Multiplex Human Phospho-ErbB3 (HER3) (Tyr1289 & Total) Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Multiplex Human Phospho-ErbB3 (HER3) (Tyr1289 & Total) Detection Kit, Control Lysate
Molecular Devices Fluorescence Filter, 625 nm, 35 nm BW
Replacement fluorescence emission filter supplied with a mounting holder for use in compatible Molecular Devices microplate readers. The filter provides a red-region emission band centered at 625 nm with a 35 nm full bandwidth, suitable for fluorescence intensity and time-resolved fluorescence assays.
- Center wavelength 625 nm.
- Bandwidth 35 nm full width.
- Includes mounting holder for slide insertion.
- Optimized for red-emission fluorescence detection.
- Intended for use in microplate readers that accept plug-in filters.
