Cell Function Reagents and Kits
Medchemexpress LLC HY-15621A 5mg Medchemexpress, DMA (trihydrochloride) CAS:2095832-33-8 Purity:>98%
Medchemexpress, HY-15621A 5mg DMA (trihydrochloride) CAS:2095832-33-8 DMA trihydrochloride is a fluorescent compound (λex=340 nm, λem=478 nm). Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Biolegend Cellular Senescence Kit Red
Cellular Senescence Kit Red; Reactivity: Mouse, Human; Apps: FC, Live cell imaging; Size: 100 tests
Biotium 5-CFDA (5-Carboxyfluorescein Diacetate,
5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein. Properties: Off-white solid soluble in DMSO; Store desiccated at 4°C; C25H16O9; MW: 460; [79955-27-4].
Cayman Chemical PROLI NONOate, Purity: ≥98%, CAS: 178948-42-0, 100 mg
PROLI NONOate is a NO donor. It spontaneously dissociates in a pH-dependent, first-order process with a half-life of 1.8 seconds at 37°C (pH 7.4) to liberate 2 moles of NO per mole of parent compound.{3266}
BANGS LABORATORIES INC ViaCheck 0 percent Viability Control Single Shots
ViaCheck 0 percent Viability Control Single Shots
LICORbio Sapphire700™ Stain for Cell Viability Assays and In-Cell Western™ Assay Normalization (100 L)
Sapphire700 Stain is a non-specific cell stain that accumulates in both the nucleus and cytoplasm of fixed or dead cells, but not live cells. When used to stain serial dilutions of A431 cells in 96-well plates, Sapphire700 Stain displays linearity of fluorescent signal for higher cell densities, from ~50,000 to ~250,000 cells/well. Simultaneous staining of cells with Sapphire700 and DRAQ5 (Biostatus, Ltd.) Stains expands the linear range, allowing more accurate normalization of cell number across both low and high cell densities.
Sigma Aldrich Fine Chemicals Biosciences Live Dead Cell Viability A
The Live-Dead Cell Viability Assay Kit is a quick and simple three-color assay to measure cell viability. The kit consists of Calcein-AM (stains live cells) Propidium Iodide (stains dead cells) and Hoechst 33342 (stains all cells). The kit has been optimized for 3D cell culture (spheroids human organoids and 3D matrices) and 2D cell culture (multiple cell types). The kit can be used for flow cytometry fluorescence microscopy and with fluorescence microplate readers. The kit determines viability of cells based on plasma membrane integrity and esterase activity. One kit contains reagents sufficient for 5 x 24-well plates or 12 x 96-well plates.boldAdvantages/bold 1. Tested and optimized for 3D cell culture Spheroid culture organoid culture 2D cell culture and flow cytometry.2. Easy to use and quick protocol.3. Stronger intensity of Calcein AM and Hoechst dyes compared to other products.Spectral PropertiesCalcein AM Ex/Em 490/515 nm (Green)Propidium Iodide Ex/Em 535/617 nm (Red)
Sartorius Fixable Viability Kit
The iQue Fixable Viability Kits are used to label viable from non-viable mammalian cells prior to downstream fixation and permeabilization which is required for intracellular cell staining. The dyes are cell membrane-impermeant that covalently label cell surface and intracellular amines resulting in dim surface staining of live cells and highly fluorescent staining of cells with compromised membranes. The staining is stable and the fluorescence is preserved following fixation and permeabilization.Key AdvantagesPreserve staining post fixation - Maintain fluorescence staining intensity to discriminate live and dead cells post fixation or permeabilization.Enable artifact-free analysis of intracellular targets- Monitor surface and intracellular marker expression simultaneously while reducing artifacts from dead cells.Save precious sample - Conserve samples for use in multiple downstream assays with only 20 L of cells required.
Perkin Elmer US LLC Sapphire Disk for TMA Ordinate Calibration
Sapphire Disk for TMA Ordinate Calibration
Lonza Walkersville ToxiLight Non-Destructive Cytotoxicity BioAssay Kit, 1000 Test
The ToxiLight BioAssay Kit is a bioluminescent, non-destructive cytolysis assay kit designed to measure the release of the enzyme, adenylate kinase (AK), from damaged cells. AK is a robust protein present in all eukaryotic cells, which is released into the culture medium when cells die. The enzyme actively phosphorylates ADP to form ATP and the resultant ATP is then measured using the bioluminescent firefly luciferase reaction. As the level of cytolysis increases, the amount of AK in the supernatant also increases, which results in emission of higher light intensity by the ToxiLight reagent. Because the ToxiLight BioAssay Kit exploits the fact that AK is released from cells when they die, there is no need for cell lysis (unlike many other cytotoxicity assays). Repeated samples of supernatant can therefore be taken over time without disrupting the cells themselves. This allows for kinetic analysis of cell death.