One-Step Reverse Transcription PCR Reagents

One-Step Reverse Transcription PCR Reagents
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Invitrogenâ„¢ SuperScriptâ„¢ IV One-Step RT-PCR System
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.


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Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | PCR Amplified cDNA |
Quantity | Each |
PCR Method | 1-step RT-PCR |
Detection Method | Gel Electrophoresis |
Polymerase | Platinum SuperFi |
Format | Kit |
Size (Final Product) | Up to 14 kb |
Reaction Format | Master Mix |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Hot Start | Built-In Hot Start |
Optimal Reaction Temperature | 45°C to 60°C |
Technique | 1-step RT-PCR |
Starting Material | RNA |
Invitrogenâ„¢ SuperScriptâ„¢ III One-Step RT-PCR System with Platinumâ„¢ Taq DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript III |
Final Product Type | PCR Amplified cDNA |
PCR Method | 1-step RT-PCR |
Detection Method | Gel Electrophoresis |
Polymerase | Platinum Taq DNA Polymerase |
Format | One-Step RT-PCR System |
Size (Final Product) | Up to 4.5 kb |
Reaction Format | Premixed Components |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Hot Start | Built-In Hot Start |
Optimal Reaction Temperature | 50°C |
Technique | 1-Step RT-PCR |
Starting Material | RNA |
Thermo Scientificâ„¢ Verso 1-Step RT-PCR Kit ReddyMix, with ThermoPrime Taq
Thermo Scientific Verso 1-Step RT-PCR Kit ReddyMix with ThermoPrime Taq is a complete set of RT-PCR reagents that combines everything needed for RT-PCR.

Invitrogenâ„¢ SuperScript IV UniPrime One-Step RT-PCR System
Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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GC-Rich PCR Performance | High |
---|---|
Format | 1-step RT-PCR Kit |
Reverse Transcriptase | SuperScript IV |
Size (Final Product) | Up to 13 kb |
Reaction Format | Master Mix |
Reagent Type | Reverse Transcription |
Hot Start | Built-in Hot Start |
Optimal Reaction Temperature | 50°C to 65°C |
Technique | 1-step RT-PCR |
Starting Material | RNA |
PCR Method | 1-step RT-PCR |
Multiplex Capability | Yes |
Detection Method | Gel Electrophoresis |
Applied Biosystemsâ„¢ TaqPathâ„¢ DuraPlexâ„¢ 1-Step RT-qPCR Master Mix, no ROXâ„¢
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
---|---|
Polymerase | Applied Biosystemsâ„¢ AmpliTaqâ„¢ DNA Polymerase with Hot Start |
GC-Rich PCR Performance | Medium |
Content And Storage | Store at –15 to –25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use. |
Format | Single-Tube |
Form | Liquid |
Reaction Speed | Fast or Standard |
Hot Start | Antibody-based |
Shelf Life | Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt |
For Use With (Application) | Virus Detection,Pathogen Detection,Real Time PCR (qPCR),Gene Expression |
PCR Method | 1-step RT-qPCR,Multiplex qPCR |
Product Line | TaqPathâ„¢ |
Detection Method | Primer-probe |
Invitrogenâ„¢ SuperScriptâ„¢ III One-Step RT-PCR System with Platinumâ„¢ Taq High Fidelity DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.

Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript III |
Final Product Type | PCR Amplified cDNA |
PCR Method | 1-step RT-PCR |
Detection Method | Gel Electrophoresis |
Polymerase | Platinum Taq High Fidelity |
Format | One-Step RT-PCR System |
Size (Final Product) | Up to 10 kb |
Reaction Format | Premixed Components |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Hot Start | Built-In Hot Start |
Optimal Reaction Temperature | 50°C |
Technique | 1-Step RT-PCR |
Starting Material | RNA |
Invitrogenâ„¢ SuperScriptâ„¢ One-Step RT-PCR System with Platinumâ„¢ Taq DNA Polymerase
SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.

Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystemsâ„¢ TaqPathâ„¢ DuraPlexâ„¢ 1-Step RT-qPCR Master Mix
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | Medium |
Content And Storage | Store at –15 to –25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use. |
Form | Liquid |
Concentration | 4X |
For Use With (Application) | Virus Detection,Pathogen Detection,Real Time PCR (qPCR),Gene Expression |
PCR Method | 1-step RT-qPCR,Multiplex qPCR |
Detection Method | Primer-probe |
Polymerase | Applied Biosystemsâ„¢ AmpliTaqâ„¢ DNA Polymerase with Hot Start |
Format | Single-Tube |
Passive Reference Dye | ROXâ„¢ |
Reaction Speed | Fast or Standard |
Hot Start | Antibody-based |
Shelf Life | Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt |
Product Line | TaqPathâ„¢ |
New England Biolabs, Inc. Luna Universal One-Step RT-qPCR Kit – 200 reactions
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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In the Luna One-Step RT-qPCR Kit, Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended.

Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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New England Biolabs, Inc. Luna Universal Probe One-Step RT-qPCR Kit – 200 reactions
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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In the Luna Universal One-Step Probe RT-qPCR Kit, Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended.

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LIFE TECHNOLOGIES SS IV 1-STEP RT-PCR SYS SAMPLE
12-595-010 SS IV 1-STEP RT-PCR SYS SAMPLE

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New England Biolabs, Inc. OneTaq One-Step RT-PCR Kit – 30 reactions
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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The OneTaq One-Step RT-PCR Kit offers sensitive and robust end-point detection of RNA templates. cDNA synthesis and PCR amplification steps are performed in a single reaction using gene-specific primers, resulting in a streamlined RT-PCR protocol. The kit combines three optimized mixes: OneTaq One-Step Enzyme Mix, OneTaq One-Step Reaction Mix and OneTaq One-Step Quick-Load Reaction Mix. The Enzyme Mix combines ProtoScript II Reverse Transcriptase, Murine RNase Inhibitor and OneTaq Hot Start DNA Polymerase. ProtoScript II Reverse Transcriptase is a mutant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. OneTaq Hot Start DNA Polymerase is mixture of a Hot Start Taq DNA Polymerase combined with a proof-reading DNA polymerase, resulting in high-yield amplification with minimal optimization. The OneTaq One-Step RT-PCR Kit is capable of amplifying long transcripts up to 9 kb in length.

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LGC CLINICAL DIAGNOSTICS INC HSV-1 RT PCR KIT 100 REACTIONS
NC2872527 HSV-1 RT PCR KIT 100 REACTIONS

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Enzo Life Sciences AMPIGENE 1-Step RT-PCR Kit (1000 Reactions)
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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AMPIGENE 1-Step RT-PCR Kit uses the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and PCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. Applications: PCR.

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Roche Diagnostics KAPA SYBR FAST One-Step qRT-PCR Kits - 100 x 20 uL reactions, 1 mL
KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting improvements in sensitivity, specificity, reaction efficiency, and fluorescence. The kits contain an M-MuLV reverse transcriptase, an RNase inhibitor, and a novel DNA polymerase engineered through our directed evolution technology. *This item is non returnable.

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