LAMP and Other Isothermal Amplification Technologies
Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.
Invitrogen™ SuperScript™ IV RT-LAMP Master Mix
Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions.
Invitrogen™ Lyo-ready Bst DNA Polymerase
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors.
Thermo Scientific™ phi29 DNA Polymerase (10 U/μL)
Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification.
Invitrogen™ Dry-Ready™ RT-LAMP Kit
Experience the simplicity and efficiency of the air drying process by gently heating materials at moderate temperatures in a simple ventilated oven.
Thermo Scientific™ Bsm DNA Polymerase, large fragment (8 U/μL)
Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities.
Thermo Scientific™ EquiPhi29™ DNA Amplification Kit
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).
Thermo Scientific™ EquiPhi29™ DNA Polymerase
Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution.
Thermo Scientific™ Reaction Buffer for phi29 DNA Polymerase (10X)
Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi29 DNA polymerase.
Thermo Scientific™ EquiPhi29™ DNA Polymerase Reaction Buffer (10X)
This reaction buffer is for use with EquiPhi29 DNA Polymerase. Buffer is included with purchase of the polymerase, but additional buffer is made available here to help accommodate experimental needs.
ABclonal Technology Pilot phi29 DNA Polymerase
Pilot phi29 DNA Polymerase differs from Phi29 DNA Polymerase by significantly enhancing protein thermal stability, reaction speed, and salt tolerance while retaining all the advantages of the latter. Pilot phi29 DNA Polymerase exhibits strong multiple strand displacement, continuous synthesis characteristics, and 3 -5 exonuclease activity.
Meridian Life Science Gly-Free Phi29 Polymerase -HC, 20UL
Glycerol-Free Phi29 DNA Polymerase (HC) is a highly processive DNA-dependent DNA polymerase with strong strand displacement activity, which enables efficient isothermal DNA amplification from low DNA inputs. Glycerol-Free Phi29 DNA Polymerase (HC) 3′ - 5′ exonuclease activity delivers high fidelity amplification, making it an appropriate solution for sequencing DNA template preparation. It is supplied with a 5x Reaction Buffer that contains excipients required for lyophilization. Glycerol-Free Phi29 DNA Polymerase (HC) enables flexible and scalable reaction volumes, and is suitable for applications such as rolling circle amplification (RCA) and multiple displacement amplification (MDA).
New England Biolabs, Inc. SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit – 96 reactions
The SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit utilizes Loop-Mediated Isothermal Amplification (LAMP) to detect SARS-CoV-2 nucleic acid. The kit is available for research use only and includes WarmStart Colorimetric LAMP 2X Master Mix with UDG and a primer mix targeting the N and E regions of the viral genome. Controls are provided to verify assay performance, and include an internal control primer set and a positive control template. Guanidine hydrochloride has been found to increase the speed and sensitivity of the RT-LAMP reaction and is also included.
Mascon Medical PD-Phi29 DNA polymerase, 5000U/pk
PD-Phi29 is an engineered variant of the phi29 DNA polymerase with enhanced thermostability and activity compared to wild-type enzyme. The polymerase retains its intrinsic 3´→5' exonuclease activity, allowing high-fidelity proofreading during nucleotide incorporation. PD-phi29 is ideal for isothermal DNA amplification with exceptionally low template input (~1fg DNA). Compatible formats include RCA and MDA for whole genome amplification and plasmid sequencing.
Features:
• Thermostable up to 42°C
• High yield even from ultra-low input
• Low error rate
• Robust strand-displacement processivity
Includes two tubes (enzyme + buffer); store at –15°C to –25°C. For Research Use Only.
PD-Phi29 DNA Polymerase, 0.1 mg/mL, 10,000 U/mL, 0.5 mL (5,000 U/PK)
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