Additional Cell Culture Reagents and Supplements
SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES BURKHOLDERIA CEPACIA SEL SUPP
NC3371068 BURKHOLDERIA CEPACIA SEL SUPP
Sigma Aldrich Fine Chemicals Biosciences Lipopolysaccharides from Escherichia coli O26:B6, MFCD00164401, 1 mg
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs). Lipopolysaccharides from Escherichia coli 026:B6 was used to elicit the secretion of cytokines by human PBMC, murine bone marrow dendritic cells and rat astrocytes.
Drg International Inc CHROMagar™ ESBL, dehydrated culture media, 5000 mL reconstituted
CHROMagar™chromogenic plates are easy to prepare - just add water to the dehydrated media, bring to a boil, let it cool down and pour! Use the entire pack, or if there is a need for a smaller number of plates, just a portion. CHROMagar™ products are sold in dehydrated form. DRG offers the 5L size (after reconstitution). If kept under appropriate storage temperatures, CHROMagar™ powder products have a long shelf life (2-3 years). This flexibility is essential to avoid the costly waste resulting from unused plates. This product requires a refrigerated supplement, RT412, to use properly; these are shipped free of charge along with the product. Supplements contain compounds that cannot be heated, therefore the compounds would degrade in the base if stored together for a long time.
STEMCELL Technologies ClonaCell™-CHO ACF Supplement, Size: 2.5 mL
ClonaCell™-CHO ACF Supplement is a serum-free and animal component-free supplement that is added to protein-free media in order to increase cloning efficiency and promote robust growth of CHO cells. The 40X supplement can be added to a variety of protein-free liquid or semi-solid cell culture media to increase the growth and single-cell cloning efficiency of suspension-adapted CHO cells. The supplement contains recombinant proteins and phenol red, and does not contain animal components, serum, hydrolysates, L-glutamine, or selection agents.
Sigma Aldrich Fine Chemicals Biosciences Pluronic F-127, 9003-11-6, MFCD00082049, 250G
Pluronic F-127 was used to coat a siliconized coverslip to hold an egg extract in a study. Pluronic F-127 was added to phosphate buffered saline (PBS), to lower unspecific cell and protein adhesion to a PDMS-based microfluidic device. A study reports its use as a release vehicle to transport low-dose perivascular lipopolysaccharide (LPS) on mouse vein grafts. PLGA/pluronic F127 may be used to fabricate nerve guidance conduits (NGCs) for regeneration of peripheral nerve. Fabrication of poly (lactide-co-glycolide) (PLGA) — Pluronic F127 glass composites was reported. Pluronic F-127 was used for fluorescent labeling of blood vessels, astrocytes, and neurons.
General Laboratory Products Saline, 0.9%, 99 mL, 12/cs
Wide mouth glass bottle, 12 per case. Saline is routinely used a diluent.
Sigma Aldrich Fine Chemicals Biosciences Lipopolysaccharides from Salmonella enterica serotype typhimurium purified by gel-filtration chromatography | MFCD00164404 | 5MG
Lipopolysaccharides from Salmonella enterica serotype typhimurium purified by gel-filtration chromatography | MFCD00164404 | 5MG
STEMCELL Technologies 3% Acetic Acid with Methylene Blue, Size: 100 mL
3% Acetic Acid with Methylene Blue is recommended for manual counting of nucleated mammalian cells. It will lyse red blood cell and white blood cell membranes. The remaining white blood cell nuclei will stain lightly with methylene blue.
Sigma Aldrich Fine Chemicals Biosciences Burkholderia cepacia Selective Agar
Burkholderia cepacia Selective Agar | Composition (agar, 15.0 g/L; crystal violet, 0.002 g/L; lactose, 10.000 g/L; phenol red, 0.080 g/L; sodium chloride, 5.0 g/L; sucrose, 10.0 g/L; tryptone, 10.0 g/L; yeast extract, 1.5 g/L), 500g
Invivogen c-di-AMP, 1 mg
Bis-(3'-5')-cyclic dimeric adenosine monophosphate (c-di-AMP) is a bacterial second messenger implicated in the control of cell wall metabolism, osmotic stress responses and sporulation. Detection of c-di-AMP by the host cytoplasmic surveillance pathway (CSP) is known to elicit type I IFN responses through a signaling axis that involves STING, TBK1 and IRF3.