Classical and Basal Cell Culture Media

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Gibco™ DMEM, high glucose, pyruvate

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

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Specifications

Shipping Condition	Ambient
Cell Line	HeLa, 293, Cos-7, and PC-12
Sterilization Method	Sterile-filtered
With Additives	High Glucose,Glutamine,Phenol Red,Sodium Pyruvate
Form	Liquid
Without Additives	No HEPES
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells

Gibco™ RPMI 1640 Medium, no glutamine, no phenol red

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

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Gibco™ FreeStyle™ 293 Expression Medium

An animal origin-free, serum-free, protein-free formulation, allowing for easier purification

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Content And Storage	Storage conditions: 2°C to 8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Product Type	Expression Medium
Form	Liquid
Classification	Animal Origin-free,Chemically-defined,Protein-free,Serum-free
Product Line	Freestyle
Serum Level	Serum-free

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Gibco™ McCoy's 5A (Modified) Medium, HEPES

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues.

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Gibco™ FreeStyle™ F17 Expression Medium

An animal origin-free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation.

Pricing & Availability
Specifications

Cell Line	HEK293
Culture Environment	CO₂
Purity or Quality Grade	Qualified for Further Manufacturing
Form	Liquid
Without Additives	No Glutamine
Endotoxin Level	< 1 EU/mL
Concentration	1 X
For Use With (Application)	Protein expression, viral vector production
Sterility	Sterile
Product Type	Expression Medium
Classification	Animal Origin-free,Chemically-defined,Protein-free,Serum-free
Culture Type	Suspension Cell Culture
Product Line	Freestyle
Serum Level	Serum-free

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Gibco™ MEM α, nucleosides

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.

Pricing & Availability
Specifications

Sterilization Method	Sterile-filtered
Product Type	MEM α (Minimum Essential Medium α)
Form	Liquid
Without Additives	No HEPES
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Product Line	Gibco
Serum Level	Standard Serum Supplementation

Gibco™ Advanced DMEM/F-12

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	MRC-5, SP2, Vero, WI-38 and Jurkat
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No Glutamine,No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Sterilization Method	Sterile-filtered
With Additives	High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Product Type	DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification	Animal Origin
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco
Serum Level	Reduced Serum

Gibco™ AIM V™ Medium, liquid (research grade)

AIM V (research grade) is a medium designed for the proliferation and/or manipulation of T cells, dendritic cells, and other immune cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Content And Storage	Store in refrigerator (2–8°C) and protect from light.
Purity or Quality Grade	Research Grade
Included Antibiotics	Streptomycin Sulfate, Gentamicin
With Additives	Glutamine,Phenol Red,HSA
Product Type	Serum Free Medium (SFM)
Form	Liquid
Culture Type	Mammalian Cell Culture
Product Line	AIM V
Cell Type	Monocytes, Dendritic Cells, T Cells, Hybridomas, PBMCs, Fibroblasts, Macrophages, Myeloma Cells, Lymphocytes

Gibco™ DMEM, high glucose, no glutamine, no methionine, no cystine

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.

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Gibco™ Leibovitz's L-15 Medium, GlutaMAX™ Supplement

Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate.

Pricing & Availability
Specifications

Product Type	Leibovitz's L-15 Medium
Form	Liquid
Without Additives	No HEPES,No Sodium Bicarbonate,No Glucose
pH	7.4 to 7.9
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Shelf Life	12 Months
Concentration	1 X

Gibco™ Basal Medium Eagle (BME)

Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells.

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Gibco™ Medium NCTC-109 (1X)

Suitable for suspension and monolayer culture methods

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Gibco™ BenchStable™ MEM

Gibco Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Content And Storage	Store at room temperature. Protect from light.
Form	Liquid
Without Additives	No HEPES,No Sodium Pyruvate
Manufacturing Quality	cGMP-compliant under the ISO 9001: 2015 standard
Green Features	ACT Ecolabel (Gold)
Concentration	1 X
Sterility	Sterile-filtered
With Additives	Low Glucose,GlutaMAX,Phenol Red
Product Type	MEM (Minimum Essential Medium)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	BenchStable
Serum Level	Standard Serum Supplementation

Gibco™ BenchStable™ DMEM

Gibco DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, 293, Cos-7, and PC-12
Content And Storage	Store at room temperature. Protect from light.
Form	Liquid
Without Additives	No HEPES,No Sodium Pyruvate
Manufacturing Quality	cGMP-compliant under the ISO 9001: 2015 standard
Green Features	ACT Ecolabel (Silver)
Concentration	1 X
Sterility	Sterile-filtered
With Additives	High Glucose,GlutaMAX,Phenol Red
Product Type	DMEM (Dulbecco's Modified Eagle Medium)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	BenchStable
Serum Level	Standard Serum Supplementation

Gibco™ MEM (Richter's modification), no phenol red

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Pricing & Availability

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Classical and Basal Cell Culture Media

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Gibco™ DMEM, high glucose, pyruvate Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ RPMI 1640 Medium, no glutamine, no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ FreeStyle™ 293 Expression Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ McCoy's 5A (Modified) Medium, HEPES Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ FreeStyle™ F17 Expression Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM α, nucleosides Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Advanced DMEM/F-12 Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ AIM V™ Medium, liquid (research grade) Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM, high glucose, no glutamine, no methionine, no cystine Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Leibovitz's L-15 Medium, GlutaMAX™ Supplement Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Basal Medium Eagle (BME) Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Medium NCTC-109 (1X) Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ BenchStable™ MEM Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ BenchStable™ DMEM Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM (Richter's modification), no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More