Classical and Basal Cell Culture Media

A variety of nutrient-rich solutions, including DMEM, RPMI, MEM, etc., used for maintaining and cultivating cells in laboratory settings, cell culture media plays a critical role in cell biology research, providing the necessary environment for cells to thrive and be studied in controlled laboratory conditions.

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Gibco™ Medium 199 (10X)

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

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Gibco™ DMEM, low glucose, pyruvate, no glutamine, no phenol red

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.

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Specifications

Cell Line	HeLa, 293, Cos-7, and PC-12
Form	Liquid
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Product Line	Gibco
Serum Level	Standard Serum Supplementation
Cell Type	Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells

Gibco™ DMEM/F-12, powder, HEPES

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

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Gibco™ Medium 199, HEPES

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

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Gibco™ MEM, NEAA, powder

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Content And Storage	Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture
Form	Powder
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Product Line	Gibco
Serum Level	Standard Serum Supplementation

Gibco™ VP-SFM AGT™ Medium

Gibco VP-SFM AGT Medium is a flexible-format medium that is equivalent to VP-SFM with the addition of Advanced Granulation Technology for simplified preparation.

Pricing & Availability
Specifications

Cell Line	MDCK, VERO, HEp2, BHK-21, COS-7
Included Antibiotics	No antibiotics
Product Type	Serum Free Medium (SFM)
Form	Powder (AGT)
Endotoxin Level	Low
Culture Type	Adherent Cell Culture
Product Line	Gibco
Serum Level	Serum-free

Gibco™ Medium 199, Hanks' Balanced Salts

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

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Gibco™ RPMI 1640 Medium, powder

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Content And Storage	Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture
Product Type	RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Form	Powder
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Shelf Life	36 Months From Date of Manufacture
Sterility	Sterile-filtered
Product Line	Gibco
Serum Level	Standard Serum Supplementation
Cell Type	Leukemic Cells

Gibco™ MEM α, no nucleosides

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.

Pricing & Availability
Specifications

Sterilization Method	Sterile-filtered
Product Type	MEM α (Minimum Essential Medium α)
Form	Liquid
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Product Line	Gibco
Serum Level	Standard Serum Supplementation

Gibco™ Ham's F-12 Nutrient Mix

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells.

Pricing & Availability
Specifications

Cell Line	CHO, COS-7, and rat prostate epithelial cells
Sterilization Method	Sterile-filtered
With Additives	Glutamine,Phenol Red,Sodium Pyruvate
Product Type	Ham's F-12 Nutrient Mixture
Form	Liquid
Without Additives	No HEPES
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Shelf Life	12 Months From Date of Manufacture
Concentration	1 X
Sterility	Sterile-filtered
Product Line	Gibco
Cell Type	Primary Rat Astrocytes

Gibco™ RPMI 1640 Medium, powder

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

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Gibco™ Opti-MEM™ I Reduced Serum Medium, GlutaMAX™ Supplement

Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	Sp2, AE-1, CHO, BHK-21, and HEK
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 18 months from date of manufacture
With Additives	GlutaMAX,Phenol Red
Product Type	Opti-MEM I
Form	Liquid
Classification	Animal Origin
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Shelf Life	18 Months From Date of Manufacture
Concentration	1X
Sterility	Sterile-filtered
Product Line	Gibco, GlutaMAX, Opti-MEM
Cell Type	Primary Fibroblasts

Gibco™ CTS™ OpTmizer™ One SFM

Gibco CTS OpTmizer One SFM is a novel animal-origin-free (AOF) serum-free medium (SFM) formulation developed for the growth and expansion of human T lymphocytes used in cell therapies.

Pricing & Availability
Specifications

Content And Storage	Store at 2-8°C and protect from light.
Sterilization Method	0.2 μm Sterile-filtered
With Additives	None
Form	Liquid
Without Additives	No Glutamine,No Phenol Red
Classification	Animal Origin-free,Serum-free,cGMP
Culture Type	Suspension Cell Culture,Dynamic Bioreactors
Shelf Life	12 Months
Sterility	Sterile-filtered
Product Line	CTS OpTmizer
Serum Level	Serum-free

Gibco™ Keratinocyte SFM (1X) Kit

Keratinocyte SFM contains L-glutamine.

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Gibco™ MEM, no glutamine, no phenol red

MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media.

Pricing & Availability

Classical and Basal Cell Culture Media

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Gibco™ Medium 199 (10X) Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM, low glucose, pyruvate, no glutamine, no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Medium 199, HEPES Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Medium 199, Hanks' Balanced Salts Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM α, no nucleosides Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Ham's F-12 Nutrient Mix Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Opti-MEM™ I Reduced Serum Medium, GlutaMAX™ Supplement Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Keratinocyte SFM (1X) Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM, no glutamine, no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Medium 199 (10X)

Gibco™ DMEM, low glucose, pyruvate, no glutamine, no phenol red

Gibco™ Medium 199, HEPES

Gibco™ Medium 199, Hanks' Balanced Salts

Gibco™ MEM α, no nucleosides

Gibco™ Ham's F-12 Nutrient Mix

Gibco™ Opti-MEM™ I Reduced Serum Medium, GlutaMAX™ Supplement

Gibco™ Keratinocyte SFM (1X) Kit

Gibco™ MEM, no glutamine, no phenol red