Buffer Solution, TISAB, Fisher Chemical
For Analysis of the Determination of Fluoride Ion Concentration by Fluoride Ion Electrode
Buffer Solution, pH 4.00, Color-Coded Red (Certified), Fisher Chemical
pH/temperature conversions given on label
Gibco™ PBS, pH 7.4
Balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents
Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Trimethylol Aminomethane, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Thermo Scientific™ Pierce™ 20X PBS Tween™ 20 Buffer
Prepare PBS-Tween (PBS-T) wash buffers and blocking buffers for ELISA and Western with this space-saving stock solution.
Thermo Scientific™ BupH™ Phosphate Buffered Saline Packs
Make 500mL of sodium phosphate buffer (PBS) for crosslinking and labeling reactions requiring amine-free conditions with these dry-blended powder pouches.
Gibco™ DPBS, no calcium, no magnesium
Balanced salt solution used for variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents
Tween™ 20, Fisher BioReagents™
CAS: 9005-64-5 Molecular Formula: C58H114O26 Molecular Weight (g/mol): 1227.54 InChI Key: HMFKFHLTUCJZJO-UHFFFAOYSA-N Synonym: Polyoxyethylene-20-sorbitan Monolaurate, Polyoxyethylenesorbitan monolaurate, Polysorbate 20 PubChem CID: 443314 IUPAC Name: 2-[2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy]ethyl dodecanoate SMILES: CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
Thermo Scientific™ BupH™ Carbonate-Bicarbonate Buffer Packs
Make 500mL of alkaline (pH 9.4) Na-carbonate (carb-bicarb) buffer for protein and antibody coating procedures with these dry-blended powder pouches.
Thermo Scientific™ Pierce™ 20X TBS Tween™ 20 Buffer
Prepare TBS-Tween (TBS-T) wash buffers or blocking buffers for ELISA, Western blotting and other immunoassays with this space-saving stock solution.
Tris Hydrochloride (Small White Flakes/Molecular Biology), Fisher BioReagents
CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N Synonym: Tris(hydroxymethyl)aminomethane Hydrochloride PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl
Thermo Scientific™ Pierce™ 20X TBS Buffer
Prepare Tris-NaCl buffer (TBS) used as a wash buffer and antibody diluent for ELISA, Western blotting and other immunoassays with this space-saving stock solution.
Thermo Scientific™ BupH™ Modified Dulbecco's PBS Packs
Make 500mL of Na- and K-phosphate buffered saline (D-PBS) for ELISA and Western blot diluents and wash buffers with these dry-blended powder pouches.
Thermo Scientific™ Pierce™ 20X Phosphate Buffered Saline
Prepare PBS buffers for crosslinking, biotinylation and fluorescent labeling reactions requiring an amine-free buffer with this space-saving stock solution.
Thermo Scientific™ BupH™ MES Buffered Saline Packs
Make 500mL of optimized amine- and carboxyl-free buffer for carbodiimide (EDC) crosslinking and conjugation reactions with these dry-blended powder pouches.
Thermo Scientific™ Pierce™ 20X Borate Buffer
Prepare sodium borate buffer solutions for protein modification procedures requiring amine-free buffer at alkaline pH with this space-saving stock solution.
Thermo Scientific™ BupH™ Tris Buffered Saline Packs
Make 500mL of Tris-NaCl buffer (TBS) for diluents and wash buffers in ELISA, Western blotting and other immunoassays with these dry-blended powder pouches.
Thermo Scientific™ Pierce™ 10X Tris-Glycine SDS Buffer
Quickly prepare Tris-glycine-SDS (pH 8.3) running buffer for SDS-PAGE (protein polyacrylamide gel electrophoresis) with this space-saving stock solution.
Tween™ 80, Fisher BioReagents
CAS: 9005-65-6 Synonym: Polyoxyethylene-20-sorbitan Monooleate, Polysorbate 80
Buffer-Pac Color-Coded Solutions (Certified), Fisher Chemical
CAS: 50-00-0 Molecular Formula: CH2O Molecular Weight (g/mol): 30.026 MDL Number: MFCD00163177 InChI Key: WSFSSNUMVMOOMR-UHFFFAOYSA-N PubChem CID: 712 ChEBI: CHEBI:16842 IUPAC Name: formaldehyde SMILES: C=O
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly. Dominant buffers in the human body include:
- Bicarbonate buffers
- Phosphate buffers
- Protein buffers
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Industrially, buffers and buffering agents help produce the right conditions for setting textile dyes. In fermentation processes, buffers help maintain the right growth conditions for yeast and other organisms. Buffers are commonly used in chemical analyses and pH meter calibrations.
The buffers used to calibrate pH meters may be certified and/or traceable to the National Institute of Standards and Technology (NIST). These buffers may also be color-coded for easy identification:
- Red: pH 4.0
- Yellow: pH 7.0
- Blue: pH 10.0