Crosslinking, Labeling and Protein Modification
Thermo Scientific™ Pierce™ Biotin Quantitation Kit
HABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins.
Thermo Scientific™ Pierce™ EMCH Crosslinker
Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this mid-length (11.8A) crosslinker containing maleimide and hydrazide groups.
Thermo Scientific™ Pierce™ Azido-Sugars
Study glycoproteins using in vivo metabolic labeling and chemoselective ligation to phosphine reagents with these azide-tagged sugars (GlcNAz, GalNAz, ManNAz).
Thermo Scientific™ Bond-Breaker™ Neutral pH TCEP Solution
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Thermo Scientific™ Pierce™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) No-Weigh™ Format
Thermo Scientific™ Pierce™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) No-Weigh™ Format is a water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium cyclohexane spacer arm.
Thermo Scientific™ Pierce™ PMPI Crosslinker
Short (8.7A) hydroxyl-to-sulfhydryl crosslinker; isocyanate and maleimide groups; label -OH by urethane bonds for conjugation to cysteines by thioether bonds.
Thermo Scientific™ Pierce™ SIA Crosslinker
Crosslink amines to sulfhydryls with this very short crosslinker composed of NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 3-atom (1.5A) spacer arm.
Thermo Scientific™ Pierce™ Iodination Tubes
Reliably label (iodinate) proteins and peptides with iodine-125 using glass test tubes that are pre-coated (activated) with iodination reagent.
Thermo Scientific™ Pierce™ 8M Guanidine·HCl Solution
Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.
Thermo Scientific™ Pierce™ NHS-Azide Staudinger Ligation Reagents
Activated azido compounds to derivatize proteins or amine surfaces for selective conjugation, detection or purification by phosphine-modified probes.
Thermo Scientific™ Pierce™ Sulfo-NHS Crosslinker
Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.
Thermo Scientific™ Pierce™ Traut's Reagent
2-Iminothiolane-HCl (CAS 4781-83-3); an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking.
Thermo Scientific™ Pierce™ SMCC Crosslinker
Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of a stabilizing cyclohexane-containing, 8-atom spacer arm.
Thermo Scientific™ Pierce™ DMP Crosslinker
Crosslink amines to amines with dimethyl pimelimidate (CAS 58537-94-3); composed of imidoester groups and a membrane-permeable, 7-atom spacer arm.
Thermo Scientific™ EZ-Link™ Psoralen-PEG3-Biotin
Biotinylate DNA or RNA via intercalation and conjugation with thymine- and other pyrimidine-containing bases with this light-activated biotinylation reagent.
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin and Labeling Kits
Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.
Thermo Scientific™ EZ-Link™ Hydrazide-PEG4-Biotin
Biotinylate glycoproteins (aldehydes of oxidized sugars) with this mid-length (31.3A), pegylated biotinylation reagent containing a 4-unit PEG spacer for solubility.
Thermo Scientific™ Pierce™ SPDP Crosslinker
Crosslink amines to sulfhydryls with this crosslinker composed of NHS-ester and pyridyldithiol groups on 3-atom spacer arm; forms cleavable disulfide bonds with cysteines.
Thermo Scientific™ Pierce™ Sulfo-SANPAH Crosslinker
Crosslink amines to anything with this long crosslinker composed of Sulfo-NHS-ester and nitrophenyl azide reactive groups; photoactivates with UV light (Amax 320 to 350nm).
Thermo Scientific™ EZ-Link™ Amine-PEG2-Biotin
Biotinylate proteins or surfaces using EDC or other amine-reactive crosslinkers with this short (20.4A) biotinylation reagent containing a 2-unit polyethylene glycol spacer.
BD 7-AAD Staining Solution
Convenient, ready-to-use nucleic acid dye that can be used in place of propidium iodide (PI)
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin No-Weigh™ Format
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin No-Weigh™ Format is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
Thermo Scientific™ Pierce™ BMOE Crosslinker
Crosslink sulfhydryls to sulfhydryls (cysteine residues in proteins or peptides) with this short crosslinker composed of maleimide groups and a 7-atom (8.0A) spacer arm.
Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format
Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format is designed for convenient preparation of stocks that will be added to protein samples for effective reduction of disulfide bonds.
Thermo Scientific™ Pierce™ Iodoacetamide No-Weigh™ Format
Thermo Scientific™ Pierce™ Iodoacetamide No-Weigh™ Format is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization and peptide mapping.
Thermo Scientific™ Pierce™ NHS-Diazirine Crosslinkers
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific™ Pierce™ TCEP·HCl
Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).
Thermo Scientific™ EZ-Link™ TFPA-PEG3-Biotin
Biotinylate molecules without common functional groups for coupling with this efficient, light-activated biotinylation reagent based on tetrafluorophenyl azide.
Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry
Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.