Substrates for ELISA Detection

Thermo Scientific™ 1-Step™ Ultra TMB-ELISA Substrate Solution Available on GSA/VA Contract for Federal Government customers only.

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate Available on GSA/VA Contract for Federal Government customers only.

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

Thermo Scientific™ SuperSignal™ ELISA Femto Substrate Available on GSA/VA Contract for Federal Government customers only.

HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

Thermo Scientific™ 1-Step™ Turbo TMB-ELISA Substrate Solution

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Thermo Scientific™ Pierce™ TMB Substrate Kit

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Invitrogen™ CDP-Star™ Substrate (0.4 mM Ready-To-Use) with Sapphire-II™ Enhancer, Size A Non-distribution product as customer accommodation.

Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.

Thermo Scientific™ QuantaRed™ Enhanced Chemifluorescent HRP Substrate Kit

Enable sensitivity comparable to enhanced chemiluminescence (ECL) using the most sensitive fluorescent ELISA substrate for peroxidase (HRP).

Invitrogen™ eBioscience™ TMB Solution (1X) Non-distribution product as customer accommodation.

Recommended for ELISA Ready-SET-Go!™ kits. eBioscience™ TMB ELISA Substrate Solution, 1x is a sensitive chromogenic substrate supplied as single ready-to-use solution.

R&D Systems™ Substrate Reagent Pack Available on GSA/VA Contract for Federal Government customers only.

8 vials of Color Reagent A (stabilized hydrogen peroxide) and 8 vials of Color Reagent B (stabilized tetramethylbenzidine)

BD TMB Substrate Reagent Set

For use in BD OptEIA™ ELISA sets. Substrate Reagent A, which is clear and colorless, contains hydrogen peroxide. Substrate Reagent B is colorless to a very light amber solution containing 3,3′, 5,5′ tetramethylbenzidine (TMB) in an organic solvent.

Thermo Scientific™ PNPP Substrate Tablets

Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.

Thermo Scientific™ 1-Step™ PNPP Substrate Solution

An alkaline phosphatase substrate for ELISA applications

Thermo Scientific™ Pierce™ PNPP Substrate Kit

Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.

Thermo Scientific™ 1-Step™ ABTS Substrate Solution

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.

Invitrogen™ eBioscience™ ELISA Wash Buffer, 1L packets Non-distribution product as customer accommodation.

Suitable for washing ELISA plate microwells. eBioscience™ ELISA Wash Buffer is supplied in 10 packets of concentrated powder, yielding a total of 10 liters of 1x Wash Buffer.

Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit Available on GSA/VA Contract for Federal Government customers only.

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

Thermo Scientific™ 1-Step™ Slow TMB-ELISA Substrate Solution

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Thermo Scientific™ OPD Substrate Tablets (o-phenylenediamine dihydrochloride)

Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

R&D Systems™ Glo Substrate Reagent Pack Available on GSA/VA Contract for Federal Government customers only.

for chemiluminescent immunoassay development

Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)

Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.

Thermo Scientific™ QuantaBlu™ NS/K Fluorogenic Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

Thermo Scientific™ PNPP Substrate

Powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble alkaline phosphatase substrate for use in ELISA applications.

Thermo Scientific™ Pierce™ Stable Peroxide Substrate Buffer (10X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

Thermo Scientific™ OPD (o-phenylenediamine dihydrochloride)

Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

Invitrogen™ Galacton-Star™ Substrate (50X Concentrate) Non-distribution product as customer accommodation.

Concentrated chemiluminescent substrate for E.coli β-galactosidase assays. Thermo Scientific Galacton-Star Substrate (50X Concentrate) is highly sensitive which makes it ideal for detection of weak expression and for transfection normalization with o

Invitrogen™ Galacto-Light™ Reaction Buffer Diluent with Galacton-Plus™ Substrate Non-distribution product as customer accommodation.

Established system with extended light emission and high sensitivity for reporter gene assays. Thermo Scientific Galacto-Light Reaction Buffer Diluent and the Galacton-Plus chemiluminescent substrate solution are used with the Galacto-Light Plus Reporter

Invitrogen™ Super AquaBlue ELISA Substrate Non-distribution product as customer accommodation.

Enhanced ABTS substrate solution for ELISA which provides high signal and sensitivity, low background. Safe, stable and convenient to use. Oxidized by HRP-labeled probes, resulting in soluble, blue-green colored end product, whose absorption can be measured by spectrophotometers at 405nm.

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