HPLC Quality Depends on Your Sample Prep Quality
HPLC Sample Preparation Starts with Sartorius
HPLC is one of the most common and precise analytical methods in use today, and can generate reproducible and accurate results. To accomplish this, your sample must be free of particles that can cause interference during detection and block the fl ow of sample through your column.
Sample prep is typically a labor-intensive, time-consuming and often tedious task. Problems that originate with sample prep can become immediately visible on the chromatogram and may require that the entire chromatography run be repeated. Sartorius products can help to address these problems and help you to achieve higher analytical accuracy.
Approximately 80% of the problems that occur during HPLC runs can be attributed to inadequate water quality. Even water sold as “HPLC-grade” can be found to have high total organic carbon (TOC) levels.
The HPLC mobile phase must be especially pure in terms of physical and chemical properties and should not contain organic impurities or particles.
You can rely on the arium mini plus UV ultrapure lab water system with its unique Bagtank system to deliver consistently high water quality. The closed Bagtank system reliably protects the pure water from secondary contamination by atmospheric components and also prevents the formation of a permanent biofilm.
The arium mini plus can be used to produce Type 1 or Type 3 water for use with SPE, IC, LC-MS, GS-MS and HPLC testing methods.
For quantitative HPLC, standard series must be prepared with defined concentrations. Solids can be difficult to weigh, and calculating the correct dilution can become time-consuming and another source of error.
Cubis MSA laboratory balances can now be converted into individual instruments when you download Q-Apps applications to your balance. These apps are integrated into the balance software to guide you step by step through the weighing process selected. Simply select the Q-App for HPLC Standards (or choose from many other options) to increase efficiency and reliability and eliminate the middleware.
BPipetting is a key step in preparing solvents for filtration — the Tacta mechanical pipette and low-retention tips can help.
Tacta pipettes have an ergonomic design and are lightweight for easy and convenient handling. The Optilock feature provides volume adjustment flexibility and protection against accidental changes during pipetting. Pipettes may need to be adjusted frequently for various viscosities, and low-retention tips feature a repellent surface to minimize residual liquid retention.
Pipetting is a key step in preparing solvents for filtration, and the Tacta mechanical pipette with low retention tips can help.
Even this routine aspect of the sample prep process can be improved by using quality equipment that makes a positive impact on your lab budget and provides a perfect balance of convenience, dependability and intelligence.
Tacta pipettes have an ergonomic design and are lightweight for easy and convenient handling.
The Optilock feature provides both volume adjustment flexibility and protection against accidental changes during pipetting.
Pipettes may need to be adjusted frequently for various viscosities, and low retention tips feature a repellent surface to minimize residual liquid retention.
Clarification by filtration removes particles from samples, which impacts the efficiency of your HPLC column and the related reliability of your results. Typically, syringe filters are used for this step; for more than just a few samples, this process can quickly turn into a tedious and hand-straining and strenuous chore.
Use the Claristep filtration system to simultaneously filter multiple HPLC samples. The patented station is designed to filter up to eight 60 to 600μL HPLC samples without the need for power, pumps or syringes, and you only need one hand!
Claristep filters have regenerated cellulose membranes (in 0.2 or 0.45μm pore sizes) and are optimized for organic and aqueous solutions and provide maximum chemical resistance and compatibility.
Simply pipette your samples into the filter reservoirs, press to close the station lid and the samples will pass through the filters and directly into your sample HPLC vials.
Clean Sample = Clean Results
These and other Sartorius products can help to improve your HPLC sample prep and offer:
- Reduced HPLC column blockage
- Improved HPLC column sensitivity
- Better accuracy
- Fewer false-positive peaks
- Less background noise
- No leachables