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Promega BacTiter-Glo™ Microbial Cell Viability Assay Kit

Catalog No. PRZG8233 Shop All Promega Corporation Products
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Quantity:
10 mL
100 mL
10 x 10mL
10 x 100mL
For Use With (Application):
Antimicrobial Compound Activity, Antimicrobial Compounds Screening, Viability Assay, Rapid Bacterial Quantitation, Rapid ATP Detection
Viability Assay
This item is not returnable. View return policy
This item is not returnable. View return policy

Includes

BacTiter-Glo Buffer; BacTiter-Glo Substrate (lyophilized)

The BacTiter-Glo™ Microbial Cell Viability Assay is a homogeneous method for determining the number of viable microbial cells in culture based on quantitation of the ATP present.

“Add-mix-measure” format utilizes a single reagent that causes cell lysis and generation of a luminescent signal after direct addition to bacterial cells cultured in medium. The luminescent signal is proportional to the amount of ATP present, which is directly proportional to the number of viable cells in culture.

  • Generates a stable, “glow-type” luminescent signal with a half-life generally over 30 minutes
  • Detects ATP from as few as 10 bacterial cells
  • Produces results quickly—data can be recorded in five minutes or less after reagent addition
  • Requires fewer handling steps than other ATP assays, making the kit ideal for automation
  • Compatible for use with 96- or 384-well plates

  • U.S. Patent 6,602,677, Australian Patent 754312 and other patents pending.
  • The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673.

Specifications

Content And Storage -20°C
For Use With (Application) Viability Assay
For Use With (Equipment) Microplate Reader
Quantity 10 x 100mL
Sufficient For 100,000 Assays at 100μL/assay in 96-well plates, 400,000 assays at 25μL/assay in 384-well plates
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