United States Biological Corporation

United States Biological Corporation DROP OUT MIX - URACIL

Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants. Convenient Powders Useful for One-Hybrid and Two-Hybrid Systems Competitively Priced...! Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates Homogeneous: Proprietary blending technology ensures complete mixing of components Companion Products: D9500: Drop-out Base With Glucose (Powder) D9501: Drop-out Base With Raffinose (Powder) D9502: Drop-out Base With Galactose (Powder) D9510: Drop-out Base With Glucose and Agar (Powder) D9511: Drop-out Base With Raffinose and Agar (Powder) D9512: Drop-out Base With Galactose and Agar (Powder) Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder)

United States Biological Corporation Nematode Growth Medium (NGM), Powder, 500g

A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58C water bath. N1000 solution needs to completely cool to the desired temperature (58C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58C, place flask(s) onto stir plate(s). Maintain temperature at 58C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution.

United States Biological Corporation Ames Medium w/L-Glutamine, Powder, 50 L

Ames' medium was formulated to support retinal tissue in relatively short-term culture. Rabbit retina has been incubated in Ames' medium for over 2 days with its metabolism and electrical responses to light stimuli well maintained. This mixture is a medium of choice for maintaining central nervous system tissue in vitro.

United States Biological Corporation ZYMOLYASE 100T 25MG

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg

United States Biological Corporation Chick Embryo Extract, Ultrafiltrate, Liquid, 20 mL

Chick Embryo Extract, Ultrafiltrate is used as a supplement in some growth media formulations. It is prepared by blending 11-14 day old chick embryos in a balanced salt solution (3X embryo volume). The solution undergoes centrifugation to remove larger particles and debris. The supernatant is subjected to an ultrafiltration step with a 10kD MW cutoff to remove protein from the solution, producing a clear, amber liquid. This liquid is sterile-filtered.

United States Biological Corporation N1010 Nematode Growth Medium, 100X (Trace Metals Mix) (Powder)

N1010 Nematode Growth Medium, 100X (Trace Metals Mix) (Powder)

United States Biological Corporation Zymolyase 100T (Lyticase, Yeast Lytic Enzyme), 37340-57-1, 100mg

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg

United States Biological Corporation IPTG DIOXINE FREE 10G

IPTG is a carbohydrate used to induce b-galactosidase for the selection of recombinant plasmids. Used to select for lac Y mutants and to induce the lac operon in E. coli. IPTG will also induce the cellular content of lactose permease. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in E. coli. Solubility: Colorless, clear, complete Identity (IR): Conforms to structure pH (5%): 5.0-7.0 Dioxane: Not Detected Specific Rotation (C=1,H2O): -29° to -35° Melting Point: 109 -114.0°C Water (KF): ≤1.0% Blue-White Assay: Induces b-galactosidase in a pUC 18-containing strain. Storage and Stability: Powder is very stable at RT. Stable for 12 months after receipt. For long-term storage of reconstituted product, aliquot and store at -20°C. Further dilutions can be made in assay buffer.

United States Biological Corporation 037167-PE Rabbit Anti-ITPRIPL1, NT (ITPRIPL1, KIAA1754L, Inositol 1,4,5-trisphosphate receptor-interacting protein-like 1) (PE)

ITPRIPL1 (inositol 1,4,5-triphosphate receptor-interacting protein-like 1), also known as KIAA1754L, is a 555 amino acid protein belonging to the ITPRIP family. ITPRIPL1 is a single-pass type I membrane protein expressed as two isoforms produced by alternative splicing events.

United States Biological Corporation DULBECCO MEM HIGH GLUC 25L

Dulbecco’s MEM DMEM High Glucose w/o Cystine and Glycine Powder; 25L; Appearance: Light tan to orange, homogeneous, free-flowing powder; UNITED STATES BIOLOGICAL mfg# D9800-19

United States Biological Corporation RNA MS2 Phage (Ribonucleic Acid) | 5ul

RNA MS2 Phage (Ribonucleic Acid) | 5ul

United States Biological Corporation X/GAL 40MG PER ML/DMF 10ML EA

Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white. Gene Cloning: In gene cloning, X-gal is used as a visual indication of whether a cell expresses a functional ²-galactosidase enzyme in a technique called blue/white screening. This method of screening is a convenient way of distinguishing a successful cloning product from other unsuccessful ones. The blue/white screening method relies on the principle of ±-complementation of the ²-galactosidase gene, where a fragment of the lacZ gene (lacZ±) in the plasmid can complement another mutant lacZ gene (lacZ”M15) in the cell. Both genes by themselves produce non-functional peptides, however, when expressed together, as when a plasmid containing lacZ± is transformed into a lacZ”M15 cells, they form a functional ²-galactosidase.

United States Biological Corporation BSA 100G

Albumin, Bovine Serum, Fatty Acid Free, is our highest purity albumin specifically designed for use in all applications, including Immunology, Tissue Culture and Microbiology. Produced in the USA. Applications include Immunohematology, Serology, RIA, Blood Banking, ELISA Blocking and Protein Control formulation. Appearance: White to tan powder Albumin: 98% Fatty Acid: 0.05% IgG: None Detected pH: 6.7-7.3 Moisture: 5% Protease: None Detected Endotoxin: 3EU/mg Conductivity (10%): 3000uS Sterility: Filtered before lyophilization through a 0.2um membrane filter. Sodium (10%): As reported Chloride (10%): As reported Calcium (10%): As reported Iron (10%): As reported Vesicular Stomatitis Virus: Negative Bluetongue Virus: Negative Microbial Growth: 3CFU/ml Country of Origin: United States Certificate of Origin: All raw material used in the manufacturing of Bovine Serum Albumin, Fatty Acid Free is of United States origin and was manufactured in USDA-registered plants.

United States Biological Corporation BACTO YEAST EXTRACT 500G

Aqueous extract of brewers yeast with an exceptionally high vitamin content. Selected for greater solubility and clarity. Free amino acid content of approximately 25%. Yeast Extract is produced from autolyzed yeast cells and is very soluble in water. It is used as an enrichment in a large number of culture media for general bacteriology and in media for sterility according to the USP. Because of its high content of carbohydrates, Yeast Extract should not be used in fermentation studies. Typical Amino Acid Profile: Ala 42%, Arg 27%, Asp 59%, Cys 5%, Glu 105%, Gly 27%, His 12%, Ile 28%, Leu 42%, Lys 45%, Met 9%, Phe 24%, Pro 21%, Ser 27%, Thr 28%, Trp 6%, Tyr 21%, Val 36% Appearance: Light yellow to medium tan, homogeneous, free flowing powder Solubility: Light yellow to amber, clear, complete pH: 6.5 � 0.5 Ash: 17% Salt: 0.5% Nitrogen (Amino): 5% Nitrogen (AN/TN): 36% Microbiological Testing: Salmonella, Standard Plate Count, Coliform (MPN/g), Viable Yeast & Mold.

United States Biological Corporation L BROTH 'LURIA' BROTH 500G

L-Broth (Luria Broth) is the standard nutrient media for propagation of Escherichia coli for purposes of strain maintenance, cloning, plasmid propagation, and protein expression. LB Broth media formulations have been industry standards for the cultivation of Escherichia coli since the 1950s (1,2,3). �These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins (4,5). �The media are nutrient-rich formulations which provide peptides and peptones, vitamins, and trace elements. �The three formulations (Lennox, Miller and Luria) differ in the amount of sodium chloride, thus providing selection of the appropriate osmotic conditions for the particular bacterial strain and desired culture conditions. The low salt formulations, Lennox and Luria, are ideal for cultures requiring salt-sensitive antibiotics such as Zeocin.