United States Biological Corporation

United States Biological Corporation Drop-out Mix Synthetic, Adenine Rich (2g) Minus Tryptophan w/o Yeast Nitrogen Base (DO, Dropout, Powder) | 100g

Drop-out Mixes are used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants.

United States Biological Corporation Nematode Growth Medium (NGM), Powder, 500g

A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58C water bath. N1000 solution needs to completely cool to the desired temperature (58C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58C, place flask(s) onto stir plate(s). Maintain temperature at 58C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution.

United States Biological Corporation TRIS BASE ULTRAPURE 1KG

Tris is one of the most widely used buffers in molecular biology and cell culture due to its low toxicity, stability and buffering capacity. Purity: ≥99.9% Appearance: White, crystalline powder pH (1M): 10.5-11.5 pKa: 8.0-8.4 Absorbance (260nm): ≤0.06 Absorbance (280nm): ≤0.05 Absorbance (290nm): ≤0.2 Solubility (1M): Colorless, clear, complete Loss on Drying: ≤0.2% Melting Point: 168-172°C Heavy Metals (Pb): ≤0.0001% Arsenic: ≤0.0001% Calcium: ≤0.0001% Copper: ≤0.0001% Iron: ≤0.0005% Magnesium: ≤0.0001% Insoluble Matter: ≤0.005% Residue on Ignition: ≤0.1% RNase: DNase: Protease: Meets specifications.

United States Biological Corporation YEAST NITROGEN BASE 500G

Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae. Y2030 has the same formulation as YNB without Amino Acids except that ammonium sulfate as a source of nitrogen has been omitted to test the ability of yeast cells to assimilate different nitrogen sources (i.e. Potassium Nitrate). Application: For carbon assimilation tests in the characterization and identification of yeasts. Note: The addition of a carbon source like glucose, galactose or raffinose is required.

United States Biological Corporation IPTG DIOXANE FREE 5G

IPTG is a carbohydrate used to induce b-galactosidase for the selection of recombinant plasmids. Used to select for lac Y mutants and to induce the lac operon in E. coli. IPTG will also induce the cellular content of lactose permease. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in E. coli. Solubility: Colorless, clear, complete Identity (IR): Conforms to structure pH (5%): 5.0-7.0 Dioxane: Not Detected Specific Rotation (C=1,H2O): -29° to -35° Melting Point: 109 -114.0°C Water (KF): ≤1.0% Blue-White Assay: Induces b-galactosidase in a pUC 18-containing strain. Storage and Stability: Powder is very stable at RT. Stable for 12 months after receipt. For long-term storage of reconstituted product, aliquot and store at -20°C. Further dilutions can be made in assay buffer.

United States Biological Corporation DROP OUT BASE/GALACTUSE/100GR

Drop-out Mix Synthetic Minus Tryptophan w/o Yeast Nitrogen Base (DO, Dropout, Powder)

United States Biological Corporation YEAST NITRO BASE 250G

Yeast Nitrogen Base w/o AA Carbohydrate & w/AS YNB Powder - 250g

United States Biological Corporation DROP OUT MIX - LEUCINE 100G

Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants. Convenient Powders Useful for One-Hybrid and Two-Hybrid Systems Competitively Priced...! Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates Homogeneous: Proprietary blending technology ensures complete mixing of components Companion Products: D9500: Drop-out Base With Glucose (Powder) D9501: Drop-out Base With Raffinose (Powder) D9502: Drop-out Base With Galactose (Powder) D9510: Drop-out Base With Glucose and Agar (Powder) D9511: Drop-out Base With Raffinose and Agar (Powder) D9512: Drop-out Base With Galactose and Agar (Powder) Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder)

United States Biological Corporation USP AGAR POWDER 1KG

Bacteriological agar is a highly purified extract of select Gelidium marine algae, forming part of the cell wall, that gives excellent growth characteristics used in a wide variety of microbiological applications. Agar is a phycocolloid, a complex mixture of several sugar compounds that retains a gelatinous structure at RT after boiling. Bacteriological agar is purified by successive washings to remove or extensively reduce chemical contaminants, color and salts. After autoclaving, agar is light amber and free of particulates, solidifying into a clear, firm gel. Agar is suitable for all molecular biology applications (i.e. bacterial and phage growth, M13 and Lambda). It is superior to standard microbiology grade agars. Appearance: White to light tan, homogenous, free flowing powder. Solubility: Light tan, clear, complete after autoclaving Moisture: 15% Ash: 6% pH (1.5%, post autoclaving): 7.2 � 1.5 Gel Strength (1.5%): 600-1200g/cm2 Gel Point (1.5%): 34-38 �C

United States Biological Corporation (DMEM) W/O AMINO ACIDS, GLUCOS

D9800-27 Dulbecco’s MEM (DMEM) w/o Amino Acids, Glucose (Powder)

United States Biological Corporation Ames Medium w/L-Glutamine, Powder, 25 L

Ames' medium was formulated to support retinal tissue in relatively short-term culture. Rabbit retina has been incubated in Ames' medium for over 2 days with its metabolism and electrical responses to light stimuli well maintained. This mixture is a medium of choice for maintaining central nervous system tissue in vitro.

United States Biological Corporation Polymannuronic Acid, Sodium Salt CAS: 29894-36-8

Sodium polymannuronate is produced from alginates by partial hydrolysis and chromatography of brown algae such as Laminaria digitata, Ascophyllum nodosum and Fucus spp. Food additive; anti-tumoral.

United States Biological Corporation Nonidet-P40 substitute, 9036-19-5, 500mL

Reagent Grade, Molecular Weight 603, Nonidet-P40 is an anhydrous liquid nonionic surface- active agent produced by the reaction of octyl phenol with 8.5-9.5 moles of ethylene oxide. Nonidet-P40 (NP-40) is a nonionic surfactant used in the isolation of membrane complexes. This product has been reformulated to be eco-friendly. The only observable differences are that the viscosity and handling characteristics are somewhat modified. Due to its nonionic structure, this product is compatible with anionic surfactants and is stable in the presence of acids, bases, and salts. It should not be mixed with concentrated oxidizing or reducing agents since the mixture of these compounds with organic compounds could form a potentially explosive mixture. Nonidet-P40 is an effective emulsifier for solvents such as xylene.

United States Biological Corporation Drop-out Mix Synthetic, Minus Methionine and Uracil w/o Yeast Nitrogen Base (DO, Dropout, Powder) 100g

Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics.

United States Biological Corporation Ames Medium w/L-Glutamine, Powder, 50 L

Ames' medium was formulated to support retinal tissue in relatively short-term culture. Rabbit retina has been incubated in Ames' medium for over 2 days with its metabolism and electrical responses to light stimuli well maintained. This mixture is a medium of choice for maintaining central nervous system tissue in vitro.