Histology Reagents
Electron Microscopy Sciences Glutaraldehyde Aqueous 10%, EM Grade 10 ML
OCHCH₂CH₂CH₂CHO F.W. 100.12
Aqueous glutaraldehyde, the universal fixative for electron microscopy, is purified to EM grade from commercial grade by "all-glass vacuum-distillation". It is the highest possible quality, free from polymers and other contaminents. Each lot is assayed prior to shipping assuring consistent purity and the highest possible quality.
If stored properly, our Glutaraldehyde has a very long shelf life. EM grade aqueous glutaraldehyde, stored in volumes greater than 100 ml, with repeated defrosting and recapping, will polymerize in time, producing an absorption peak of 235nm rather than the 280nm of pure EM grade. The presence of polymerized glutaraldehyde greatly reduces its efficiency as a cross-linking agent, preventing proper fixation. For this reason, we provide 10 ml ampoules sealed under dry nitrogen and 100 ml bottles that are kept refrigerated until it is shipped.
Grade: EM Grade
Electron Microscopy Sciences Alcian Blue 0.5% 250 ML
Used in Dane's Method for Prekeratin, Keratin, and Mucin. Keratin stain method for the staining of keratin, prekeratin, acid mucopolysaccharides and nuclei. See Dane, E.T., and Herman, D.L.: Stain Technology, 38:97, 1963., or AFIP Manual of Histological Staining Methods, 3rd Ed., Ed L. Luna: New York: McGraw Hill Publications, c 1968, p.83.
Electron Microscopy Sciences Acid Alcohol 1% 500 ML
Used in Ehrlich's Hematoxylin , Mallory (1938); Ehrlich (1886), Nuclei.
Electron Microscopy Sciences 0.2% KOH in 80% Alcohol 500 ML
Alkaline Alcohol Formulation is available for your convenience in a concentration of 0.2% KOH in 80% Alcohol.
Polysciences, Inc. Picric acid, formalin and acetic acid fixative. Bouin's fixative is excellent for use in preserving soft and delicate tissue structures.
Picric acid, formalin and acetic acid fixative. Bouin's fixative is excellent for use in preserving soft and delicate tissue structures.
Sigma Aldrich Fine Chemicals Biosciences Formalin solution, neutral buffered, 10% case of 24 x 60 mL, histological tissue fixative | MFCD00003274 | 1CS
Formalin solution, neutral buffered, 10% case of 24 x 60 mL, histological tissue fixative | MFCD00003274 | 1CS
Electron Microscopy Sciences Orange G Solution 500 ML
Used in Dane's Method for Prekeratin, Keratin, and Mucin. Keratin stain method for the staining of keratin, prekeratin, acid mucopolysaccharides and nuclei. See Dane, E.T., and Herman, D.L.: Stain Technology, 38:97, 1963., or AFIP Manual of Histological Staining Methods, 3rd Ed., Ed L. Luna: New York: McGraw Hill Publications, c 1968, p.83.
Electron Microscopy Sciences Propylene Glycol, 85% Solution 500 ML
Used in Oil Red O Method for Lipofuscin, Pigment stain. See references Luna, L.G., (ED.), manual of Histologic Staining Methods of the AFIP, 3rd Edition. The AFIP, 3rd Edition, McGraw-Hill, NY, p 186, c 1968.
Electron Microscopy Sciences Glutaraldehyde Aqueous 8%, EM Grade 10 ML
OCHCH₂CH₂CH₂CHO F.W. 100.12
Aqueous glutaraldehyde, the universal fixative for electron microscopy, is purified to EM grade from commercial grade by "all-glass vacuum-distillation". It is the highest possible quality, free from polymers and other contaminents. Each lot is assayed prior to shipping assuring consistent purity and the highest possible quality.
If stored properly, our Glutaraldehyde has a very long shelf life. EM grade aqueous glutaraldehyde, stored in volumes greater than 100 ml, with repeated defrosting and recapping, will polymerize in time, producing an absorption peak of 235nm rather than the 280nm of pure EM grade. The presence of polymerized glutaraldehyde greatly reduces its efficiency as a cross-linking agent, preventing proper fixation. For this reason, we provide 10 ml ampoules sealed under dry nitrogen and 100 ml bottles that are kept refrigerated until it is shipped.
Grade: EM Grade
Electron Microscopy Sciences Eosin Y 0.1% Aqueous 500 ML
Used in Maximow's Method for Bone Marrow, Morphology details of marrow cells. Mallory (1961). Morphology details of marrow cells. See AFIP Manual of Histologic Staining Methods, 3rd Ed, Ed. L. Luna: New York: McGraw Hill Publications, c. 1968, p. 120 or Mallory, F.B., Pathological technique, New York, Hafner Publishing Co., 1961, p.196
Electron Microscopy Sciences Bouin's Fixative 500 ML
Used in Masson's Trichrome for Connective Tissue for stain nuclei, agentaffen granules, collagen, cytoplasm, keratin, etc. References: Sheehan and Hrapchak: Theory and Practice of Histotechnology, St. Louis. The Moxby Company 1980, p. 190
Electron Microscopy Sciences 20% Paraformaldehyde (formaldehyde) aqueous solution, 100 mL
Paraformaldehyde - methanol free solution. A more efficient and rapid penetrant fixative used in combination with Glutaraldehyde and Acrolein fixatives. 100 ml bottles.
Electron Microscopy Sciences Formalin 10% Buffered in Acetate Buffer, 6.0 to 7.1 pH at 20°C pH 1 LT
Buffered with Sodium Acetate in accordance with the A.F.I.P. manual of Histology. Filtered and ready-to-use. pH 6.0 to ph 7.1 at 20 degree C
pH: 6.0 to 7.1 pH at 20°C
Electron Microscopy Sciences Ammonia Water 0.1% 500 ML
Used in Hirano-Zimmerman Method for Nerve Cells and Fibers for staining of nerofibrils, dendrites, axis cylinders, senile plaques, etc. See AFIP Manual of Histological Staining Methods, 3rd ed., Ed. L.Luna: New York: McGraw-Hill Pub., c 1968, p. 198 or Hirano, A. and Zimmerman, H.M., Arch. Neurol., 6:114-122, 1962
Electron Microscopy Sciences Glutaraldehyde Aqueous 25%, Biological Grade 2 450 ML
OCHCH₂CH₂CH₂CHO F.W. 100.12
Aqueous glutaraldehyde, the universal fixative for electron microscopy, is purified to EM grade from commercial grade by "all-glass vacuum-distillation". It is the highest possible quality, free from polymers and other contaminents. Each lot is assayed prior to shipping assuring consistent purity and the highest possible quality.
If stored properly, our Glutaraldehyde has a very long shelf life. EM grade aqueous glutaraldehyde, stored in volumes greater than 100 ml, with repeated defrosting and recapping, will polymerize in time, producing an absorption peak of 235nm rather than the 280nm of pure EM grade. The presence of polymerized glutaraldehyde greatly reduces its efficiency as a cross-linking agent, preventing proper fixation. For this reason, we provide 10 ml ampoules sealed under dry nitrogen and 100 ml bottles that are kept refrigerated until it is shipped.
Grade: Biological 2