Used the Maldonado's Method for the staining of alpha, beta, D- and Exocrine cells of the islets of langerhans. (Bouin's Fixation without Acetic Acid ). See references Maldonado, R. and San Jose, H. Stain Techn., 42:11-13, 1967 and Luna , L.G., (ed.), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw, N.Y. p. 105, c. 1968