Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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271 – 285 of 3,556 results

271

TRIS, MP Biomedicals™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.14 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM, Tris-(hydroxymethyl) aminomethane,Trometamol

Pricing & Availability
Specifications

CAS	77-86-1
Molecular Weight (g/mol)	121.14
Synonym	2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM, Tris-(hydroxymethyl) aminomethane,Trometamol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

272

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ACES, Biological Buffer, 98%, Spectrum™ Chemical

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273

Thermo Scientific™ Phosphate-buffered saline (DPBS, 1X), Dulbecco's formula, low endotoxin

Pricing & Availability
Specifications

Packaging	Plastic bottle
Physical Form	Liquid
Recommended Storage	Ambient temperatures

274

MilliporeSigma™ Bicine, ULTROL™ Grade, Calbiochem™,

CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M Synonym: N,N-bis-(2-Hydroxyethyl)glycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: sodium 2-[bis(2-hydroxyethyl)amino]acetate SMILES: [Na+].OCCN(CCO)CC([O-])=O

Pricing & Availability
Specifications

PubChem CID	8761
CAS	150-25-4
Molecular Weight (g/mol)	185.16
ChEBI	CHEBI:40957
MDL Number	MFCD00004295
SMILES	[Na+].OCCN(CCO)CC([O-])=O
Synonym	N,N-bis-(2-Hydroxyethyl)glycine
IUPAC Name	sodium 2-[bis(2-hydroxyethyl)amino]acetate
InChI Key	MFBDBXAVPLFMNJ-UHFFFAOYSA-M
Molecular Formula	C6H12NNaO4

275

Bicine, MP Biomedicals

CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M Synonym: bicine,n,n-bis 2-hydroxyethyl glycine,diethylolglycine,dihydroxyethylglycine,diethanol glycine,n,n-di 2-hydroxyethyl glycine,bicene,2-bis 2-hydroxyethyl amino acetic acid,n,n-dihydroxyethyl glycine,n,n-dihydroxyethylglycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]acetic acid SMILES: [Na+].OCCN(CCO)CC([O-])=O

Pricing & Availability
Specifications

PubChem CID	8761
CAS	150-25-4
Molecular Weight (g/mol)	185.16
ChEBI	CHEBI:40957
MDL Number	MFCD00004295
SMILES	[Na+].OCCN(CCO)CC([O-])=O
Synonym	bicine,n,n-bis 2-hydroxyethyl glycine,diethylolglycine,dihydroxyethylglycine,diethanol glycine,n,n-di 2-hydroxyethyl glycine,bicene,2-bis 2-hydroxyethyl amino acetic acid,n,n-dihydroxyethyl glycine,n,n-dihydroxyethylglycine
IUPAC Name	2-[bis(2-hydroxyethyl)amino]acetic acid
InChI Key	MFBDBXAVPLFMNJ-UHFFFAOYSA-M
Molecular Formula	C6H12NNaO4

276

PIPES, 99.4%, MP Biomedicals™

CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N Synonym: Piperazine-N, N'-bis[2-ethanesulfonic acid] PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

Pricing & Availability
Specifications

PubChem CID	79723
CAS	5625-37-6
Molecular Weight (g/mol)	302.36
ChEBI	CHEBI:44933
SMILES	C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
Synonym	Piperazine-N, N'-bis[2-ethanesulfonic acid]
IUPAC Name	2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	IHPYMWDTONKSCO-UHFFFAOYSA-N
Molecular Formula	C8H18N2O6S2

277

Gibco™ DPBS, no calcium, no magnesium

Gibco™ Balanced Salt Solutions are sterile-filtered, packaged in bioprocess containers, and ready to order.

Pricing & Availability
Specifications

Color	Clear, colorless solution
Physical Form	Liquid
pH	7.0 to 7.3
Manufacturing Quality	cGMP
Chemical Name or Material	DPBS
Osmolality	270 to 300 mOsm/kg
Sterility	Sterile-filtered

278

MP Biomedicals™ Phosphate Buffered Saline (PBS), Dulbecco's formula, without Magnesium and Calcium

DPBS Powder

Pricing & Availability

279

MilliporeSigma™ HEPES, Free Acid, ULTROL™ Grade, 1.0 M Solution, Calbiochem™,

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

Pricing & Availability
Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
IUPAC Name	2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

280

MilliporeSigma™ Tris Hydrochloride, OmniPur™, Calbiochem™,

CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N Synonym: 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride; 1,3-Propanediol, 2-amino-2- (hydroxymethyl)-, hydrochloride (1:1); 2-Amino-2-hydroxymethyl-1,3-propanediol hydrochloride; Tris(hydroxymethyl)aminomethane, hydrochloride; Tris hydrochloride PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl

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Specifications

PubChem CID	93573
CAS	1185-53-1
Molecular Weight (g/mol)	157.594
SMILES	C(C(CO)(CO)N)O.Cl
Synonym	1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride; 1,3-Propanediol, 2-amino-2- (hydroxymethyl)-, hydrochloride (1:1); 2-Amino-2-hydroxymethyl-1,3-propanediol hydrochloride; Tris(hydroxymethyl)aminomethane, hydrochloride; Tris hydrochloride
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
InChI Key	QKNYBSVHEMOAJP-UHFFFAOYSA-N
Molecular Formula	C4H12ClNO3

281

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HEPES Sodium Salt, Biological Buffer, High Purity, 98%, Spectrum™ Chemical

CAS: 75277-39-3 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.28 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M IUPAC Name: sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1

Pricing & Availability
Specifications

CAS	75277-39-3
Molecular Weight (g/mol)	260.28
SMILES	[Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1
IUPAC Name	sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate
InChI Key	RDZTWEVXRGYCFV-UHFFFAOYSA-M
Molecular Formula	C8H17N2NaO4S

282

Gibco™ Tris Base Solutions

Gibco concentrates are intended for dilution at the point of use and come sterile-filtered and packaged in conveniently sized bioprocess containers. Sourcing concentrated stock solutions can help streamline buffer preparation by helping reduce preparation time, storage space, and the manufacturing footprint required for hydration.

Pricing & Availability
Specifications

Percent Purity	Not Available
CAS	77-86-1
For Use With (Equipment)	ILC/ILD System
Molecular Weight (g/mol)	Raw Material: 121.14
Packaging	Bioprocess Container in Secondary Packaging
Physical Form	Solution
pH	10.0 to 12.0
Manufacturing Quality	CGMP
Grade	EP/USP
For Use With (Application)	Bioproduction
Solution Type	Buffer

283

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MOPS Hemisodium Salt, Biological Buffer, Spectrum™ Chemical

CAS: 117961-20-3 Molecular Formula: C14H29N2NaO8S2 Molecular Weight (g/mol): 440.50 InChI Key: OVCWZKQPQOVIDT-UHFFFAOYSA-M IUPAC Name: sodium 3-(morpholin-4-yl)propane-1-sulfonic acid 3-(morpholin-4-yl)propane-1-sulfonate SMILES: [Na+].OS(=O)(=O)CCCN1CCOCC1.[O-]S(=O)(=O)CCCN1CCOCC1

Pricing & Availability
Specifications

CAS	117961-20-3
Molecular Weight (g/mol)	440.50
SMILES	[Na+].OS(=O)(=O)CCCN1CCOCC1.[O-]S(=O)(=O)CCCN1CCOCC1
IUPAC Name	sodium 3-(morpholin-4-yl)propane-1-sulfonic acid 3-(morpholin-4-yl)propane-1-sulfonate
InChI Key	OVCWZKQPQOVIDT-UHFFFAOYSA-M
Molecular Formula	C14H29N2NaO8S2

284

R&D Systems™ 10X PBS

The 10X PBS (phosphate buffered saline) buffer is a high quality concentrated solution manufactured without magnesium or calcium, and is suitable for cell resuspension and washing in the in situ apoptosis detection (TUNEL) procedure, CometAssay™ (Cat No. 4250-50-K) applications, and any other experiment requiring ultrapure PBS buffer.

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285

MilliporeSigma™ HEPES, Free Acid, OmniPur™, Calbiochem™,

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

Pricing & Availability
Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
Synonym	N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid
IUPAC Name	2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

Life Science Buffers

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ACES, Biological Buffer, 98%, Spectrum™ Chemical Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

HEPES Sodium Salt, Biological Buffer, High Purity, 98%, Spectrum™ Chemical Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

Gibco™ Tris Base Solutions Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

MOPS Hemisodium Salt, Biological Buffer, Spectrum™ Chemical Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

ACES, Biological Buffer, 98%, Spectrum™ Chemical

HEPES Sodium Salt, Biological Buffer, High Purity, 98%, Spectrum™ Chemical

Gibco™ Tris Base Solutions

MOPS Hemisodium Salt, Biological Buffer, Spectrum™ Chemical