Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Quality Biological Inc Tris (1M), pH 9.8 1000 mL
Suitable for molecular biology protein chemistry and biochemical applications.
DNase RNase and Protease tested.
Filtered through a 0.22 µm membrane
Applications: Tris Buffer (1M), pH 9.8 is commonly used in SDS-PAGE and Electrophoresis applications.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase RNase and Protease Tested
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Quality Biological Inc Tris-HCl (2M), pH 6.8 500 mL
Tris-HCl Buffer is compatible with many enzymes in molecular biology (e.g. DNA modifying enzymes). Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959).
Use the Tris-HCl 0.5 M (pH 6.8) buffer for:
— stacking gels
— resolving gels
— DEAE Dextran-mediated transfection of non-adherent and adherent cells
Applications: Tris-HCl (2M), pH 6.8 is useful with SDS-PAGE and Electrophoresis.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase RNase and Protease Tested
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-25.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 25 Grams
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
![Aat Bioquest HHBS [Hanks Buffer with 20 mM Hepes] (100 mL)](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502249795.JPG-250.jpg)
Aat Bioquest HHBS [Hanks Buffer with 20 mM Hepes] (100 mL)
Hanks Balanced Salt Solution with 20 mM Hepes buffer HHBS is used for a variety of cell culture applications such as washing cells transporting cells or tissue samples diluting cells for counting and preparing reagents. This catalog is for 100 mL.
![Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 50 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75030-50.0.JPG-250.jpg)
Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 50 Grams
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
Research Products International Corp PIPES FREE ACID ULTRAPURE, 10GM
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Research Products International Corp PIPES FREE ACID ULTRAPURE, 100GM
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Research Products International Corp Phosphate Buffered Saline, 5 L
PBS 10X Solution is a concentrated phosphate-buffered saline widely used in cell biology, immunohistochemistry, and molecular biology. It contains phosphate ions that maintain physiological pH and ionic strength, creating a stable environment for cells, tissues, and biomolecules. The 10X concentration allows for easy dilution to 1X for various applications such as washing, resuspending, or preparing samples. The phosphate buffer system resists pH changes, ensuring experimental consistency. PBS 10X is a reliable, versatile reagent that supports reproducible results in biological and biochemical workflows.
STEMCELL Technologies RoboSep™ Human CD11b Positive Selection and Depletion Kit, For processing: 1 x 10^9 cells
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips
Research Products International Corp MES Sodium Salt 5 KG
MES Sodium Salt, also known as 2-(N-Morpholino)ethanesulfonic acid sodium salt, is a zwitterionic buffer commonly used in biological and biochemical research.
In the life science field, MES Sodium Salt is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 5.5 to 6.7, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MES Sodium Salt is commonly used in protein purification, enzyme assays, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MES Sodium Salt is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes.;
Research Products International Corp MES Anhydrous 5 KG
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
Research Products International Corp BES 500 G
For use in preparing electrophoresis buffers. pKa (25° C) 7.1. The working pH Range in aqueous solution is 6.4-7.8
