Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld TE (Tris-EDTA) Buffer, Sterile, 100 mL
Product DescriptionTE (Tris-EDTA) is a sterile buffer that has a pH level of 8.0 brought up with HCl and is stored at room temperature. It contains 10 mM Tris and 1 mM EDTA. It is of biotechnology grade and no DNAse activity is detected.TE buffer is used to solubilize DNA or RNA while protecting it from degradation. Commonly used for DNA isolation and electrophoresis protocols.
Bioworld TBE Buffer (25X), 4 L
Product DescriptionTBE (Tris-Borate-EDTA) has a liquid concentrate of 25X, where 1X contains 0.089M Tris, 0.089M Borate, and 0.002M EDTA. 1L of concentrate makes 25L of 1X buffer. Tris solutions are effective buffers for slightly basic conditions. Borate, a derivative of boric acid, is a weak proton donor but a Lewis acid that can accept an electron pair. EDTA is a chelator of divalent cations, mainly Mg2+. TBE is most commonly used for nucleic acid electrophoresis.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Bioworld TBE Sample Buffer (6X), 50 mL
Product DescriptionTBE (Tris-Borate-EDTA) is a sample buffer used for nucleic acid analysis. It has a 6X concentration, 270 mM Tris base, 270 mM boric acid, 40% glycerol, 0.025% bromophenol blue G, 0.025% xylene cyanol, and 6 mM of EDTA.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Bioworld TAE Buffer (50X), 500 mL
Product DescriptionTAE (Tris-Acetate-EDTA) buffer liquid concentrate has a 50X concentration and is filtered through a 0.22 µm membrane. It has a pH of 8.5 ± 0.1. A concentration of 1X contains 0.04M Tris, 0.02M acetate, and 0.001M EDTA. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
![Research Products International Corp RPI PIPES [Piperazine-N-N'-bis(2-ethanesulfonic acid)], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P40140-5000.0.JPG-250.jpg)
Research Products International Corp RPI PIPES [Piperazine-N-N'-bis(2-ethanesulfonic acid)], 5 Kilograms
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
![Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 98.6g make, 1 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661308.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 98.6g make, 1 L
PBS [Phosphate Buffered Saline] 10X Powder Concentrate is a white granular powder that, when dissolved in water, creates a 10X concentrated PBS solution. This 98.6g pack makes 1 liter of 10X PBS, providing a convenient and efficient way to prepare large volumes of phosphate-buffered saline. The powder dissolves quickly and is ideal for laboratories that need a concentrated PBS solution for dilutions or experiments that require higher concentration buffers. PBS is widely used in cell culture, molecular biology, and protein studies, and the 10X concentrate is ideal for reducing storage space and simplifying buffer preparation in the lab.
Research Products International Corp HEPES, Free Acid, 25 KG
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
Research Products International Corp TE Buffer 20X Solution pH 8.0, 250 Milliliters
Formulation:
EDTA: 20 mM
Research Products International Corp TRIS BUFFERED SALINE, 10X SOLUTION, 10 LITER
TBS 10X Solution (Tris Buffered Saline) is a concentrated buffered salt solution containing Tris and sodium chloride. It maintains pH stability and provides ionic strength for biological experiments. Tris acts as a buffering agent to keep pH consistent, while sodium chloride mimics physiological conditions. Dilute 10X solution with distilled water to prepare working concentrations (e.g., 1X). TBS is widely used in protein and nucleic acid blotting, immunohistochemistry, and molecular biology techniques, ensuring stable and reproducible results across diverse applications.
Research Products International Corp TRIS-Tricine-SDS Running Buffer 10X Solution, 1 Liter
10X Formulation:
Tricine: 1 M
SDS (pH 8.3): 1%
Alkali Scientific QuickSilver™ Buffer Powder, Tris-Glycine-SDS Buffer Powder, 50 pouches
QuickSilverª Buffer Powder Packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, easy to use, and come in a variety of buffers for Nucleic Acid and Protein Electrophoresis, Western Blotting, ELISA, and Cell Culture. Quick and Economical (and low shipping costs!) Convenient premeasured pouches Each pouch makes 1L of 1X buffer No pH adjustment needed Nuclease and protease free Quality and consistency guaranteed Long shelf life Buffers for Electrophoresis of Nucleic Acids and Proteins: TBE, TAE, Fast Running Buffer, Tris-Glycine-SDS, Tris-MOPS-SDS, Tris-MES-SDS Buffers for Western Blotting, ELISA and Cell Culture: TBS, TBST, PBS, PBST
Alkali Scientific CellPro™ DPBS 10X, 500ml
Balanced salt solution used for multiple cell culture applications, (liquid form). Certificate of Analysis available electronically. Standard stock items from 100 mL to 200 L Volumes up to 1000 L available as made to order Not for diagnostic or therapeutic use. For making isotonic solutions for cell or tissue rinsing, cell dilution, reagent prep, transportation etc. where maintenance of cell integrity is required for further manufacturing or research use.
Enzo Life Sciences Wash buffer concentrate, (100ml)
Formulation: Liquid. TRIS buffered saline containing detergents. Long Term Storage: Ambient. Applications: ELISA .
National Diagnostics TBE BUFFER (10X) 1 LT
TBE Buffer (10X), Formulated with 18MegOhm water. 0.2 micron filtration. The most stable TBE on the market, 1 LT
