Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp MES Sodium Salt [2-(N-Morpholino)ethanesulfonic acid] sodium salt, 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M22050-500.0.JPG-250.jpg)
Research Products International Corp MES Sodium Salt [2-(N-Morpholino)ethanesulfonic acid] sodium salt, 500 Grams
MES Sodium Salt, also known as 2-(N-Morpholino)ethanesulfonic acid sodium salt, is a zwitterionic buffer commonly used in biological and biochemical research.
In the life science field, MES Sodium Salt is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 5.5 to 6.7, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MES Sodium Salt is commonly used in protein purification, enzyme assays, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MES Sodium Salt is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.4, RNase Free) - 250ml
Tris-HCl Buffer (1 M pH 7 4 RNase Free) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X without K, pH 7.4) - 1L
Phosphate Buffered Saline PBS (10X without K pH 7 4) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc PIPES Buffer (1 M, pH 7.0) - 500ml
PIPES Buffer (1 M pH 7 0) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc MES-SDS Buffer (20X) - 4L
MES-SDS Buffer (20X) - 4L (For Research Use and Further Manufacturing Only)
Supply Solutions Red Blood Cell Lysing Buffer Hybri-Max™, 100mL
Recommended for use by adding 1 mL of buffer to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is incomplete, steps 1-4 may be repeated.
Rockland Immunochemicals 1.0 M Tris HCl pH 7.6 - MB-003
Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.
Rockland Immunochemicals 10X TE pH 8.0 (100 mM Tris HCl 10 mM EDTA) DEPC TREATED - MB-007
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
Rockland Immunochemicals 1.0 M Tris HCl pH 8.0 - MB-005
Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.
Biotium Fish Gelatin
Fish gelatin is commonly used in blocking solutions to minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Because this gelatin is derived from the skin of cold water fish it does not cross-react with mammalian antibodies. Fish gelatin can be used in blocking buffers to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures.
Biotium Ready-to-Use Tyramide Amplification Buffer, 1X, 25ML
Ready-to-use tyramide amplification buffer can be used for tyramide signal amplification with CF Dye Tyramides or other tyramides without the need to add hydrogen peroxide.
Thermo Scientific Production Chemicals and Services 50MM TRIS 10 MM EDTA 1.65M AM
NC3588257 50MM TRIS 10 MM EDTA 1.65M AM
Thermo Scientific Production Chemicals and Services 50 MM TRIS-HCL 10 MM EDTA PH
NC3588258 50 MM TRIS-HCL 10 MM EDTA PH