Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC 2-Morpholinoethanesulphonic acid monohydrate | 145224-94-8 | 99.95% | 250 MG
MES monohydrate is an analytical standard intended for research and analytical applications. It is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely used in biochemistry and molecular biology experiments as a reference standard for assays.
- Used in cell culture
- Used in enzyme activity determination
- Used in electrophoresis
- Used in protein studies
- Commonly used in qualitative, quantitative, and methodological research experiments in HPLC, GC, and MS
Bioworld BES, 1M, pH 7.0, 100 mL
BES bufferAlso known as N,N-Bis(hydroxyethyl)-2aminoethanesulfonic acid.Technical Information:pKA (25 C): 7.1Heavy Metals: <0.0005%. Appearance: LiquidBoiling Point : 404.34 °C (Predicted)Density : 1.44 g/cm3 (Predicted)Melting Point: 152-154 °CPH Value: 6.4-7.8
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 500 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255318.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 500 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurine ACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agent inbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+. Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 7.0
Chem-Impex International, Inc. Piperazine-N,N'-bis(2-ethanesulfonic acid) 1.5 sodium salt | 100037-69-2 | MFCD00066393 | 250G
Piperazine-N,N'-bis(2-ethanesulfonic acid) 1.5 sodium salt, 100037-69-2, MFCD00066393, 250G
![Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660042.jpg-250.jpg)
Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 250 G
MES Monohydrate (2-(N-Morpholino) ethanesulfonic acid) in a 250g volume is a biochemical reagent designed for pH buffering in biological and chemical experiments. MES is widely used for maintaining a stable pH in laboratory applications such as protein analysis, enzyme assays, and cell culture. This specific size is ideal for smaller volume experiments where larger quantities are unnecessary. MES provides a precise and consistent pH environment, making it invaluable in research involving electrophoresis, chromatography, and other biochemical processes. Its non-interfering nature with biological systems ensures that it does not affect the results of sensitive assays and experiments, especially in molecular biology.
![Alkali Scientific PIPES Disodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid)disodium salt], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659783.jpg-250.jpg)
Alkali Scientific PIPES Disodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid)disodium salt], 500 G
PIPES Disodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) disodium salt) is a highly reliable biochemical reagent used in buffer solutions to maintain pH stability in a variety of laboratory experiments. This 500g quantity is perfect for large-scale buffer preparation in protein analysis, enzyme assays, and microbiological testing. PIPES disodium salt provides a stable pH of approximately 7.0, which is essential for maintaining biological activity during cellular studies and biochemical research. Its strong buffering capacity ensures consistent pH regulation, making it an ideal choice for applications in molecular biology, protein purification, and other sensitive laboratory experiments requiring precise pH control.
![Alkali Scientific POPSO, Free Acid, [Piperazine N N' bis(2 hydroxypropane sulfonic acid)], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660132.jpg-250.jpg)
Alkali Scientific POPSO, Free Acid, [Piperazine N N' bis(2 hydroxypropane sulfonic acid)], 25 G
POPSO (Free Acid), Laboratory Grade, is available in a 25g package and is used in a wide range of biochemical and molecular biology applications. It is commonly used as a buffering agent to maintain a stable pH environment during protein analysis, enzyme assays, and other scientific procedures. POPSO is known for its effectiveness in maintaining physiological pH ranges, making it ideal for cell culture, biochemical reactions, and analytical studies. The reagent is available in a convenient 25g size, perfect for smaller experiments and academic research, ensuring reliable performance in maintaining pH stability during laboratory work.
![eMolecules 467454-51-9 | tert-butyl 1-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | Pharmablock | MFCD24466941 | 213.277 | C11H19NO3 | 97.000 | CC(C)(C)OC(=O)N1CC2CC1(CO)C2 | 1g | 586155718](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502158706.JPG-250.jpg)
eMolecules 467454-51-9 | tert-butyl 1-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | Pharmablock | MFCD24466941 | 213.277 | C11H19NO3 | 97.000 | CC(C)(C)OC(=O)N1CC2CC1(CO)C2 | 1g | 586155718
tert-butyl 1-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | Pharmablock | 467454-51-9 | MFCD24466941 | 213.277 | C11H19NO3 | 97.000 | CC(C)(C)OC(=O)N1CC2CC1(CO)C2 | 1g | 586155718
LAB BIOREAGENTS LowBinding I Buffer 500ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
Crescent Chemical Co Inc PROTEIN TEST MIX 4 FOR SDS PG
Protein Test Mixture 4 for SDS PAGE 4 Dalton-marker proteins, high molecular weight, for SDS PAGE Molecular weight markers for SDS gel electrophoresis. The bovine albumin (BSA) contains monomers and oligomers.Reconstitute with SDS sample buffer (125 mM Tris-HCl pH 6.8, 2 % SDS, 15 %