Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
| Percent Purity | 99.8% to 100.2% |
|---|---|
| CAS | 77-86-1 |
| For Use With (Equipment) | Instruments and Electrodes |
| Molecular Weight (g/mol) | 121.135 |
| Physical Form | Crystalline Powder |
| pH | 10.0 to 11.5 for 5% solution |
| Grade | ACS |
| For Use With (Application) | Laboratory |
| Solution Type | Standard |
Tris(hydroxymethyl)aminomethane, ACS Reagent (Tris; THAM; 2-Amino-2-(hydroxymethyl)-1,3-propanediol), Reagents
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.14 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tromethamine IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: NC(CO)(CO)CO
| CAS | 77-86-1 |
|---|---|
| Molecular Weight (g/mol) | 121.14 |
| SMILES | NC(CO)(CO)CO |
| Synonym | Tromethamine |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Lonza™ AccuGENE™ PBS
Ready to use solutions ideal for a wide range of molecular biology applications
| pH | 7.4 |
|---|---|
| Quantity | 1000mL |
| Recommended Storage | 18° to 26°C |
| For Use With (Application) | Ideal for a wide range of molecular biology applications |
| Recommended Storage | 18° to 26°C |
|---|
GVS™ Dilution Bottle Butterfield's Buffer (72Pack)
Butterfield’s Buffer in 90 mL or 99 mL sterile bottles for 1:10 dilutions and general use.
| pH | 7.2 |
|---|---|
| Chemical Name or Material | Butterfield's Buffer |
Phosphate Buffered Saline 47, Gold Standard Diagnostics
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Ready-to-use buffer solution for AIX1000 workflows, supporting consistent assay performance and reliable diagnostic results.
GVS™ Dilution Bottle Phosphate Buffer Magnesium Chloride 99mL
Phosphate Buffer with magnesium chloride is used as the diluents for the preparation of dilutions in plate counts in the dairy and food industries. Recommended by ALHA for the recovery of injured Microorganisms from dairy and food samples. For use in BOD testing of water and wastewater.
AccuGENE™ MOPS, 10X Buffer Concenrate, Lonza™
Specially formulated 10X buffer ensuring lot-to-lot consistency for RNA electrophoresis with precast gels.
YSI™ Nitrate Calibration Solutions
Extremely accurate calibration solutions to calibrate field instruments equipped with a nitrate sensor
| Quantity | 1 Ea. |
|---|
| Formulation | Tris Glycine-SDS Buffer, 10X with 0.25M Tris base, 1.92M Glycine, 1% SDS |
|---|---|
| Physical Form | Liquid |
| Chemical Name or Material | Protein Sample Loading Buffer |
| Recommended Storage | 18° to 26°C |
| Concentration | 10X |
AccuGENE™ 1X TE Buffer, Lonza™
A ready to use solution ideal for a wide range of molecular biology applications
Citrate Buffer (pH 6.0), Concentrate
Citrate buffer is used in Heat Induced Epitope Retrieval (HIER) methods.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Wash Buffer for GeneJET Gel Extraction Kit
Thermo Scientific™ GeneJET Wash Buffer is a component of the GeneJET Gel Extraction Kit and may be purchased separately. Provided in 45 mL aliquots.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Tris EDTA buffer, 1X, Molecular Biology Grade, Promega™
10mM Tris-HCl containing 1mM EDTA-Na2.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Recommended Storage | 15 to 30°C |
|---|