Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova MES Elution Buffer, pH 6.5. 1000mL, Sterile
MES solution is a zwitterionic buffer used to help maintain pH levels in biochemistry experiments. It has a buffering range of pH 5.8 - 6.5.
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660279.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 100 G
HEPES, Free Acid (N-(2-Hydroxyethyl) Piperazine-N'-(2-Ethanesulfonic Acid)) is a biochemical reagent commonly used as a buffering agent in biological and chemical research. The free acid form of HEPES is highly effective at maintaining pH stability in biological assays, particularly in environments where pH needs to be tightly controlled. This 100g package size is ideal for smaller laboratories or experiments that require high-purity HEPES for preparing buffer solutions. HEPES is widely used in molecular biology, cell culture, and microbiology for its low interference in biological reactions, ensuring accurate and reproducible results in a variety of applications.
![Alkali Scientific HEPPS [N (2 Hydroxyethyl)piperazine N' (3 propane sulfonic acid)] [EPPS], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660370.jpg-250.jpg)
Alkali Scientific HEPPS [N (2 Hydroxyethyl)piperazine N' (3 propane sulfonic acid)] [EPPS], 100 G
HEPPS (N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)) is a biochemical reagent available in a 100g bottle. This buffering agent is commonly used in laboratory settings to maintain a stable pH in aqueous solutions, particularly in biochemistry and molecular biology experiments. HEPPS is frequently used for enzyme assays, protein purification, and cell culture applications where precise pH control is necessary. It has a stable buffering capacity and works well at physiological pH levels, making it ideal for maintaining pH balance in a variety of biological assays. This reagent is essential for accurate experimental results in numerous biochemical processes.
Boston Bioproducts Inc TAPS Buffer (1 M, pH 8.5) - 100ml
TAPS Buffer (1 M pH 8 5) - 100ml (For Research Use and Further Manufacturing Only)
Research Products International Corp TAPS 1 KG
pKa(25°:C): 8.4. The working pH range in aqueous solution is 7.7-9.1.
![Research Products International Corp TAPS, [Tris(hydroxymethyl)-3-aminopropanesulfonic acid], Free Acid, 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T12000-500.0.JPG-250.jpg)
Research Products International Corp TAPS, [Tris(hydroxymethyl)-3-aminopropanesulfonic acid], Free Acid, 500 Grams
pKa(25°:C): 8.4. The working pH range in aqueous solution is 7.7-9.1.
Teknova 1M MOPS, pH 7.0. 1000mL, Sterile. Endotoxin Tested. 2 pack.
MOPS (3-(N-morpholino)propanesulfonic acid) is a Good’s buffer and has a pKa of 7.01, making it an ideal buffer for many biological systems at near-neutral pH. MOPS can be used in cell culture media and biopharma buffer formulations as well as in the purification of antibodies, peptides, proteins and blood components. It is nontoxic to cell culture lines.
Alkali Scientific Bicine Ultra Pure, 500 G
Bicine Ultra Pure in a 500g size is a high-purity biological buffer, formulated for optimal performance in a wide range of laboratory applications. This buffer is commonly used in protein assays, electrophoresis, and DNA/RNA work due to its excellent buffering capacity within the pH range of 7.6–9.0. The 500g package is ideal for high-throughput research laboratories that require a reliable and cost-effective source of buffer for large-scale operations. Bicine's minimal background interference and low ionic strength make it perfect for sensitive applications. This product ensures consistent pH regulation, vital for experimental reproducibility in molecular biology, biochemistry, and pharmaceutical research.
![Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92040-500.0.JPG-250.jpg)
Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 500 Grams
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
Research Products International Corp PIPES Sesquisodium Salt, 1 KG
PIPES Sesquisodium Salt, also known as PIPES Na3, is a chemical compound that has significant applications in the life science marketplace. PIPES stands for piperazine-N,N'-bis(2-ethanesulfonic acid), and the sesquisodium salt refers to the trisodium salt form of PIPES.
It is primarily utilized as a buffering agent. Buffers are substances that help maintain a stable pH in biological and biochemical processes, ensuring optimal conditions for various enzymatic reactions and cellular activities.
PIPES Sesquisodium Salt is particularly valuable in biological research, molecular biology, and cell culture applications. It offers excellent buffering capacity in the physiological pH range (6.1 - 7.5), making it suitable for a wide range of experimental conditions.
Sigma Aldrich Fine Chemicals Biosciences HEPES | 7365-45-9 | MFCD00006158 | 250 g
HEPES | Purity: ≥99.5% | Mol Wt: 238.30 | 7365-45-9 | MFCD00006158 | 250 g
Research Products International Corp Bicine Ultra-Pure, 500 Grams
Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.
pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C
Medchemexpress LLC MES sodium salt (2-Morpholinoethanesulphonic acid sodium salt) | 71119-23-8 | 98.0% | 1 ML
MES sodium salt (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. It is a Good's buffer widely used in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Buffer capacity ranging from pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments
- Effective buffering capacity around pH 6.15
- Poor affinity with metal ions
- Suitable for studies involving metal ions
Medchemexpress LLC MOPS | 1132-61-2 | 99.9% | 100 G
MOPS is a buffering agent used in biology. MOPS buffer maintains the pH of mammalian cell culture media. It also significantly improves the thermal stability of BSA (HY-D0842).