Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific Neutralizing Buffered Peptone Water (NBPW), Sterile, Pre Enrichment Medium with Disinfectant Neutralizers, Ready to Use (Bottled) Microbiology Diluent, 400mL, 10/Case
Neutralizing Buffered Peptone Water (NBPW), Sterile, Pre Enrichment Medium with Disinfectant Neutralizers, Ready to Use (Bottled) Microbiology Diluent, 400mL, 10/Case. This diluent is ideal for neutralizing disinfectants in microbiological applications, ensuring the safety and accuracy of microbial testing. It provides a sterile and ready-to-use solution to support your lab's needs. For use in microbiological analysis and laboratory protocols, this product is designed to provide reliable results.
Andwin Scientific VWR PBS-T BUFFER POWDER 100/CS
VWR® Buffer Powder Packs can save time and money, as there is no need to measure and weight many separate chemical components which is required when making buffers from scratch.Buffer powder quickly dissolves to make 1L of 1X solutionNo weighing or measuring of separate componentsNo pH adjustments requiredEconomical shipping, long shelf lifeQuality and consistency guaranteedVWR® Pre-measured buffer packs are extremely easy to use and are formulated so each pack makes 1 Liter of 1X buffer. Simply tear open a pack and mix completely with 1 liter of distilled water.Shipping costs are also minimal, as each case of 50 or 100 packs is compact and weighs only 2.5 Lbs.
Rockland Immunochemicals 10X PBS pH 7.2 with Azide (0.2 M Potassium Phosphate 1.5 M Sodium Chloride 0.1% (w/v) Sodium Azide) - MB-011
Phosphate Buffered Saline (PBS) is a regularly used buffer in biological research. The phosphate ions help maintain a constant pH around neutral. The osmolarity of solution typically matches that of the human body (isotonic solution). PBS can act as a diluent for many experimental preparations and is non-toxic to cells. This preparation contains 0.1% sodium azide as a preservative and is 10X concentrated to be diluted 10-fold to the working concentration.
Rockland Immunochemicals 10X TE pH 7.5 (100 mM Tris HCl 10 mM EDTA) DEPC TREATED - MB-006
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
Teknova 1X PBS Solution, pH 7.4, 1000mL, Sterile. 2 Pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova 1M Tris- HCl, pH 9.0 Solution. 100mL. Sterile. 10 pack.
1M Tris- HCl, pH 9.0 Solution. 100mL. Sterile. 10 pack.
Teknova 10mM Tris-HCl, pH 8.0, 20mL in 50mL Tube, 25 Tubes per Pack, DNase/RNase/Protease Tested, Sterile. 4 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Crystalgen Inc PBS PH7.2 10X 500ML
PBS (Phosphate Buffered Saline) pH7.2 10X, with Ca, Mg-500mlUltra pure Phosphate buffered isotonic saline. With calcium and magnesium. Sterile filtered. 1X PBS solutions contains 137mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 2mM KH2PO4.pH: 7.2Storage : 18-26oC
Axxora LLC Hepes Buffer 1 M pH 7.5
Hepes Buffer is one of the best general purpose buffers available for biological research. It has been used to advantage in tissue culture, oxidative phosphorylation and protein synthesis with cell-free bacterial systems. Hepes is a recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials.
Newcomer Supply Phosphate Buffer 0.1M, pH 7.2 to 7.4, 1 gallon
Use: IHC wash and rinse. Shelf Life is 1 year from date of manufacture. Store at 2-8°C.
AG Scientific Inc MOPS Free Acid 50 G
MOPS, also known as 3-(N-morpholino)propanesulfonic acid, is one of Good’s buffers. It is N-substituted amino sulfonic acid with a morpholinic ring and has zwitterionic and inert properties. MOPS buffer is broadly used in biological and biochemical studies. It can be mixed with MOPS sodium salt to attain desired pH.MOPS is recommended as an agent for non-coordinating buffers in solutions with metal ions because it hardly forms a complex with most metal ions such as Cu, Ni, Mn, Zn and Co ions. The Good's buffer interferes with the Folin protein assay and partially decomposes when autoclaved in the presence of glucose. MOPS can be used as a Good’s buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water.
Chem Service Inc Tris(1,3-dichloroisopropyl)phosphate | 13674-87-8 | MFCD00083121 | 250mg
Tris(1,3-dichloroisopropyl)phosphate | MW: 430.88 | 13674-87-8 | MFCD00083121 | 250mg
![Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 98.6g make, 1 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661308.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 98.6g make, 1 L
PBS [Phosphate Buffered Saline] 10X Powder Concentrate is a white granular powder that, when dissolved in water, creates a 10X concentrated PBS solution. This 98.6g pack makes 1 liter of 10X PBS, providing a convenient and efficient way to prepare large volumes of phosphate-buffered saline. The powder dissolves quickly and is ideal for laboratories that need a concentrated PBS solution for dilutions or experiments that require higher concentration buffers. PBS is widely used in cell culture, molecular biology, and protein studies, and the 10X concentrate is ideal for reducing storage space and simplifying buffer preparation in the lab.