Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific CellPro™ PBS 10X, pH 7.0, 500mL, 500mL, 10/case
CellPro™ PBS 10X, pH 7.0, 500mL Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
Boston Bioproducts Inc Sodium Phosphate Buffer (0.2 M, pH 7.0) - 1L
The Sodium Phosphate Buffer is a versatile reagent with a high buffering capacity, commonly used across various scientific disciplines across biochemistry and molecular biology. This buffer has a pH range of 5.8 to 8.0. It's applications include protein analysis, where it supports solubility, stability, and activity. It is also compatible with biologically relevant metal ions that are significant for cellular or physiological conditions.
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 100x1ml
Cell crude extracts contain various endogenous enzymes such as phosphatases and proteases capable of degrading proteins present in the extracts The optimal strategy to enhance intact protein yield is adding specific inhibitors targeting these enzymes This Protease Inhibitor Cocktail is used in fungal and yeast extracts to improve protein stability by inhibiting proteases responsible for degrading both phosphorylated and non-phosphorylated protein substrates This Protease Inhibitor Cocktail consists of individual compounds including AEBSF 1 10-Phenanthroline E-64 and Pepstatin A which provide broad-spectrum specific inhibition of cysteine proteases serine proteases aspartic proteases and metalloproteases It is optimized and tested for fungal and yeast extracts This cocktail is available as a ready-to-use solution dissolved in DMSO
Seracare Life Sciences Inc 10X DULBECCO S PBS (D-PBS)
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications as well as tissue and cell culture procedures. This modified Dulbecco's buffer is not formulated with magnesium or calcium salts. pH=7.4. Conductivity controlled.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Alkali Scientific CellPro™ DPBS Buffer, 1X, with Calcium and Magnesium, 500mL, 10/Case
Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.
Scytek Laboratories ScyTek Phosphate Buffered Saline (25x), 7.6 pH, 1000ml
ScyTek Phosphate Buffered Saline (25x) pH of 7.6 is an optimal formulation of pH stabilizers and salts designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal. Our Wash buffers do not contain hazardous preservatives such as Azide or Mercury that may interfere with antibody-antigen binding interactions. For your convenience wash buffer is offered in a wide variety of formulations to meet the needs of your specific ELISA application. This product can be customized to meet the specific needs of your assay. Inquire about custom vialing, labeling, kit assembly and drop shipping.
Apexbio Technology LLC Annexin V-Cy3/SYTOX Green Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane lipid bilayer but flips from the inner to the outer leaflet during the early stages of apoptosis becoming exposed to the extracellular environment Annexin V has a high affinity for PS By conjugating Annexin V with Cy3 the labeled Annexin V serves as a fluorescent probe enabling detection of apoptosis events via flow cytometry or fluorescence microscopy within only 10 20 minutes staining SYTOX Green is a membrane-impermeable cyanine dye that normally cannot penetrate intact cell membranes However during late-stage apoptosis or necrosis SYTOX Green can cross disrupted regions of the cell membrane reach the nucleus and bind DNA double helix molecules forming SYTOX Green-DNA complexes Binding increases fluorescence intensity over 500-fold generating bright green fluorescence Thus SYTOX Green stains o
Apexbio Technology LLC One-step TUNEL Cy5 Apoptosis Detection Kit 20rxns
One-step TUNEL Cy5 Apoptosis Detection Kit is designed for detecting cell nuclear DNA fragmentation in tissues or cells undergoing apoptosis During apoptosis intracellular DNA endonucleases are activated cleaving genomic DNA between nucleosomes and resulting in DNA fragmentation into segments approximately 180-200 bp or multiples thereof The fragmented genomic DNA contains exposed 3 -OH ends which are labeled with fluorescent-tagged dUTP in a TdT (Terminal Deoxynucleotidyl Transferase) catalyzed reaction allowing visualization and detection by fluorescence microscopy or flow cytometry (Ex/Em 649 nm/670 nm) This kit has a broad range of applications and can be utilized to detect apoptosis in frozen or paraffin-embedded tissue sections as well as in cultured adherent or suspension cells
Med Vet International Lisinopril Usp Tablets, 20mg, 100ct
VET LICENSE NEEDS TO BE PROVIDED IN 3-4 BUSINESS DAYS OR ORDER WILL BE CANCELLED
Lisinopril is used to treat high blood pressure (hypertension) in adults and children who are at least 6 years old. Lisinopril is also used to treat congestive heart failure in adults, or to improve survival after a heart attack.
Alkali Scientific CellPro™ DPBS Buffer, 1X, with Calcium and Magnesium, 500mL
CellPro™ DPBS Buffer, offered in a 1X concentration and a convenient 500mL volume, provides a balanced salt solution essential for numerous cell culture and molecular biology procedures. Its molecular biology grade ensures high purity and reliability in maintaining cellular health and integrity during experiments.
Ethos Biosciences Dilurex 1X PBS Buffer, 100ml, Ethos
Dilurex 1X PBS is a ready-to-use working concentration of pH 7.4 phosphate buffered saline. Skip the tedium of weighing powders and stocking raw materials that can expire. Our PBS is convenient, ready to use, and will save you hours in the lab, letting you focus on breakthroughs rather than buffer prep. Thousands of researchers trust Ethos Biosciences 1X PBS for its proven track record in top-tier publications and protocols - upgrade your productivity today.