Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture | 7365-45-9 | MFCD00006158 | 1kg
HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culturePurity: | MW: 238.3 | 7365-45-9 | MFCD00006158 | 1kg
Sigma Aldrich Fine Chemicals Biosciences PIPES | 5625-37-6 | MFCD00006159 | 100 g
PIPES | Mol Wt: 302.37 | 5625-37-6 | MFCD00006159 | 100 g
General Laboratory Products 2mL 0.85 per. Saline, 100/cs
2mL 0.85 per. Saline with Shelf Life of 1 yr.
eMolecules 205535-92-8 | Ambeed | Benzyl (2-(2-(2-hydroxyethoxy)ethoxy)ethyl)carbamate | 250mg | 660585595 | A898378 | MFCD07357495 | 283.324 | C14H21NO5
Ambeed | 6-Chlorochroman-4-one | 1g | 552578805 | A142130 | 37674-72-9 | MFCD00024070 | 182.600 | C9H7ClO2
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Medchemexpress LLC 1H-1,2,3-Triazole, 1-(3-iodophenyl)-4-(3-nitrophenyl)- | 1809031-84-2 | 99.5% | 392.15 | 10 MG
P62-mediated mitophagy inducer (PMI) is a P62-mediated mitophagy activator that activates mitochondrial autophagy without recruitment of Parkin or collapse of the mitochondrial membrane potential. It remains active in cells lacking a fully functional PINK1/Parkin pathway and serves as a pharmacological tool to study the molecular mechanisms of mitosis, avoiding toxicity and some of the non-specific effects associated with the sudden dissipation of mitochondria lacking membrane potential.
- P62-mediated mitophagy activator
- Activates mitochondrial autophagy without recruitment of Parkin or collapse of the mitochondrial membrane potential
- Remains active in cells lacking a fully functional PINK1/Parkin pathway
- Serves as a pharmacological tool to study the molecular mechanisms of mitosis
- Avoids toxicity and some non-specific effects associated with the sudden dissipation of mitochondria lacking membrane potential
ILEX LIFE SCIENCES LLC TRYPSIN 10X P10-022100 6X100ML
PAN-Biotech (10x) Trypsin 2.5 % in PBS, w/o: Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech (10x) Trypsin 2.5% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in a 10x concentrated PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 2.50% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Alkali Scientific MES Buffer, Anhydrous, 5 Kg
MES (2-(N-morpholino)ethanesulfonic acid) anhydrous is a biochemical reagent designed for pH buffering in laboratory applications. This 5kg volume of MES anhydrous is used to prepare MES buffers that provide a stable pH range, particularly for biological assays and biochemical studies. MES is widely used in enzyme reactions, protein stabilization, and tissue culture media where maintaining a consistent acidic pH is critical. It is particularly effective at buffering in the pH range of 5.5 to 6.7, making it suitable for various biochemical processes, including those in protein chemistry and molecular biology. MES is often utilized in electrophoresis, chromatography, and other sensitive experiments requiring precise pH control.
![Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660293.jpg-250.jpg)
Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 100 G
MES Sodium Salt (2-(N-Morpholino) ethanesulfonic acid), sodium salt, in a 100g volume is a biochemical reagent designed for pH buffering in laboratory experiments. It is commonly used in biological assays, cell culture, and protein purification due to its ability to maintain a stable and non-interfering pH. The sodium salt form of MES is particularly useful in experiments where higher ionic strength is required. This 100g size is ideal for smaller experiments and research studies that need precise pH control without requiring large quantities of reagent. MES Sodium Salt is an essential component in molecular biology and biochemistry for maintaining optimal conditions in sensitive biological assays.
Apexbio Technology LLC Annexin V-PE Reagent 20tests
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane phospholipid bilayer However during the early stages of apoptosis PS moves from the inner surface to the outer surface of the cell membrane becoming exposed to the extracellular environment Labeled with a fluorescence dye such as PE Annexcin V is commonly used as a fluorescent probe to detect apoptosis through flow cytometry or fluorescence microscopy within 15-30 minutes To use this reagent additional purchase of 10X Binding Buffer (Cat No K2284) is required or you can directly purchase the Annexin V-PE Apoptosis Kit which includes the buffer (Cat No K2281) To further identify different stages of apoptosis we provide ready-to-use double-staining kits Annexin V-PE/7-AAD Apoptosis Kit (K2283) or Annexin V-PE/DAPI Apoptosis Kit (K2282)
Apexbio Technology LLC Annexin V-Cy5/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Under normal conditions PS resides on the inner side of the cell membrane lipid bilayer However in early apoptosis PS translocates from the inner leaflet of the plasma membrane to the outer surface becoming exposed externally Annexin V exhibits high binding affinity for PS and when conjugated with fluorescent molecules (Cy5) it serves as a fluorescent probe to detect apoptosis using flow cytometry or fluorescence microscopy The staining procedure requires only 10 20 minutes PI specifically binds to double-stranded DNA to produce red fluorescence it is normally impermeant to intact cell membranes However during late-stage apoptosis or in dead cells PI penetrates the membrane to stain nuclear DNA Combined Annexin V-Cy5 and PI staining allows distinction between cells at various stages of apoptosis and dead cells
Apexbio Technology LLC HEPES Sodium salt 75277-39-3 250g
HEPES Sodium salt (CAS 75277-39-3) is a zwitterionic buffering agent designed to stabilize hydrogen ion concentration and maintain physiological pH (6 8 8 2) in biological systems thereby supporting cellular function and biomolecular interactions HEPES Sodium salt exerts its biological activity primarily through non-volatile amphoteric chemical buffering In in vitro and cell-based settings HEPES Sodium salt supports cell viability and experimental consistency by minimizing pH fluctuations Based on these properties HEPES Sodium salt holds research potential in cell-based assays maintenance of cultured mammalian cells controlled in vitro experiments studies of lysosomal biogenesis and investigations of cellular metabolic mechanisms
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.5, 500 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :pKa (25 °C) :7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.5
Bioworld ACES Buffer, 0.5M, pH 7.5, 100 mL
Product DescriptionACES (N-(2-acetamindo)-2-aminoethancesulfonic acid) buffer has a concentration of 0.5M and a pH level of 7.5. Buffer was prepared in 18.2 megohms water and filtered through 0.22 micron filter. ACES has been used to develop buffers for both agarose and polyacrylamide gel electrophoresis. A saturated solution contains 0.22M at 0°C and a melting point greater than 220°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+, or Mn2+. Product is stored at room temperature.
Bioworld ADA Buffer, 0.2M, pH 6.0, 250 mL
Product DescriptionADA (N-(2-acetamido)iminodiacetic acid) buffer has a concentration of 0.2M and a pH value of 6.0. It has a molecular weight of 190.2 and formula of C6H10N2O5. ADA is a zwitterionic buffer used in biochemistry and molecular biology, such as its use in protein-free medium for supporting chick embryo fibroblasts. It has also been shown to interfere with color development in bicinchoninic acid assay (BCA) for protein quantitation and used in isoelectric focusing of proteins in immobilized pH gradients.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.