Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.8 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.8 (0.2 micron filtered), 500 ml.
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid | 60-00-4 | MFCD00003541 | 5kg
Ethylenediaminetetraacetic acid | Purity: ≥98.5%, powder | MW:292.24 | 60-00-4 | MFCD00003541 | 5kg
Alkali Scientific CellPro™ TE 1X (Tris EDTA), pH7.4, 100mL
Introducing CellPro™ TE 1X (Tris EDTA), meticulously formulated by Alkali Scientific™ to meet the exacting standards of molecular biology research. This Molecular Biology Grade TE buffer ensures exceptional purity and performance, making it an indispensable tool for various molecular biology applications, including DNA and RNA purification, DNA sequencing, and PCR. With its precisely balanced composition and stringent quality control measures, CellPro™ TE 1X provides researchers with reliable and reproducible results, facilitating accurate and efficient laboratory workflows. Trust CellPro™ TE 1X for your molecular biology experiments and experience the assurance of superior quality and performance in every assay.
Chem-Impex International, Inc. Ethylenediaminetetraacetic acid | 60-00-4 | MFCD00003541 | 100G
Ethylenediaminetetraacetic acid, 60-00-4, MFCD00003541, 100G
Pfanstiehl Inc Tris Hydrochloride (Tris HCl) High Purity, Low Endotoxin, Low Metals (T-154) 1Kg GMP CAS: 1185-53-1 Injectable Excipient Grade
Pfanstiehl Tris HCL, High Purity, Low Endotoxin, Low Metals, is a buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris HCl meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. cGMP , pharmaceutical grade .
![Research Products International Corp Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60040-500.04.JPG-250.jpg)
Research Products International Corp Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 500 Grams
Tris Base Ultra Pure is a commonly used reagent and buffer component. Tris Base, Tris(hydroxymethyl)aminomethane, is a primary amine that acts as a buffering agent to maintain a stable pH in experimental procedures.
It is particularly effective in the pH range of 7.2 to 9.0, making it suitable for applications requiring slightly alkaline to mildly acidic pH conditions. Tris Base Ultra Pure is highly purified to remove impurities and contaminants, ensuring the highest quality for laboratory use.
The ultra-pure form is essential for applications where the presence of impurities can negatively impact experimental results. Tris Base Ultra Pure is commonly used in various molecular biology techniques such as DNA and RNA extraction, protein purification, and enzymatic assays.
It is also utilized in the preparation of electrophoresis buffers and PCR reactions.
Research Products International Corp TRIS, Amino Buffer, Enzyme Grade, 5 Kilograms
- CAS Number: 77-86-1
- Molecular Formula: C4H11NO3
- Molecular Weight: 121.14 g/mol
Alkali Scientific CellPro™ Tris Glycine SDS Buffer 10X, pH 8.3, 1L
Alkali Scientific™ CellPro™ Tris Glycine offers a sterile solution ideal for a variety of biochemical and molecular biology applications. Composed of Tris (Tris(hydroxymethyl)aminomethane) and Glycine, this buffer provides optimal conditions for protein electrophoresis, particularly in SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) systems. The sterile formulation ensures the purity and integrity of your samples, minimizing the risk of contamination and ensuring reliable results. CellPro™ Tris Glycine buffer is versatile and suitable for use in protein separation, transfer, and analysis techniques, making it an essential component in the laboratory for protein research and characterization. Trust in the quality and consistency of CellPro™ Tris Glycine for your protein electrophoresis experiments.
National Diagnostics TRIS-GLYCINE-SDS PAGE BUFFER (
Tris-Glycine-SDS PAGE Buffer (10X), Ultra-pure concentrated standard running buffer for SDS-PAGE. Formulated with 18 MegOhm water. 0.2 micron filtration, 4 LT
Rockland Immunochemicals 1.0 M Tris HCl pH 9.0 - MB-028-1000
Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid anhydrous, free-flowing, powder, Redi-Dri(TM), ACS reagent, 99.4-100.6% | 60-00-4 | MFCD00003541 | 500G
Ethylenediaminetetraacetic acid anhydrous, free-flowing, powder, Redi-Dri(TM), ACS reagent, 99.4-100.6% | Purity: 99.4-100.6% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500G
Bioworld Tris-Glycine-SDS (TG-SDS) Buffer, Ready-Pack (10X), 4 PK(s)
Tris Glycine-SDS BufferTD-SDS Buffer is blended using bioPLUS reagents to provide the highest quality buffer for protein research. Ideal for protein separations, this buffer is formulated to ensure exceptional purity in the absence of nuclease and protease activities.TG-SDS Buffer is a widely used running buffer for denaturing electrophoresis of proteins (SDS-PAGE).Technical Information:Storage: RTHazard: Irritant1 pack makes 1 L of 10x concentrate.1X Solution Contains: 0.025 M Tris, 0.192 M Glycine and 0.1% SDS.
Bioworld TBE Urea Sample Buffer (2X), 50 mL
Product DescriptionTBE(Tris-Borate-EDTA) urea sample buffer is used for nucleic acid analysis. It has a 2X concentration and contains 45 mM Tris base, 45 mM Boric acid, 6% Ficol, 3.5M Urea, 0.005% Bromophenol blue, 0.025% Xylene and 1 mM EDTA.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.