Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
National Diagnostics ProtoGel Resolving Buffer (4X) 450ML
Traditional Laemmli Resolving Gel Buffer (4X). Formulated with 0.2 Micron Filtration.Ultra-Pure Reagents in 18 MegOhm Water.Crystal Clear, Reproducible Gels. Catalog number: EC-892. The use of National Diagnostics' ProtoGel Resolving Buffer will ensure the purity and performance of your Laemmli gels. When diluted as directed ProtoGel Resolving Buffer forms a gel of 0.375 M Tris-HCl and 0.1% SDS, pH 8.8. Storage: ProtoGel Resolving Buffer is stable for 24 months when stored tightly capped in a dark area at room temperature.
National Diagnostics PROTOGEL STACKING BUFFER 200 M
ProtoGel Stacking Buffer, 4X Laemmli Tris-HCl stacking gel buffer for clear, reproducible SDS-PAGE, 200 ML
National Diagnostics SEQUAGEL BUFFER 200 ML
SequaGel Buffer, System consists of SequaGel Concentrate, SequaGel Diluent, and SequaGel Buffer for casting 19:1 denaturing DNA gels of a varying percentage, 200 ML
Lonza Walkersville TheraPEAK PBS w/o Ca Mg GMP
TheraPEAK PBS serves as a wash solution in a wide variety of laboratory applications this GMP produced version fits excellent with downstream processing in cell and gene therapy and protein manufacturing applications This BioWhittaker Cell Culture Media is produced according to cGMP and labelled For Further Manufacturing (FFM) GMP Products are intended for research or further manufacturing use only THIS PRODUCT IS NOT INTENDED FOR DIRECT THERAPEUTIC USE IN HUMANS
Alkali Scientific CellPro™ Tris HCL 1M pH 8.5, 500mL
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Medchemexpress LLC HEPPS | 16052-06-5 | 99.0% | 50 G
HEPPS (EPPS) is a buffering agent with a useful pH range from 7.3 to 8.7. It reduces Aβ-aggregate-induced memory deficits and rescues cognitive deficits in mice. EPPS is orally active and penetrates the blood-brain barrier. It binds to amyloid-β aggregates and converts them into monomers, thereby reducing the formation of amyloid-β oligomers and plaques. This agent also rescues cell damage induced by amyloid-β aggregates.
- Binds to amyloid-β aggregates and converts them into monomers
- Reduces formation of amyloid-β oligomers and plaques
- Rescues cell damage induced by amyloid-β aggregates
- Improves cognitive dysfunction in APP/PS1 transgenic mice
- Reduces Aβ oligomer and plaque deposition in the brain
- Reduces glial GABA release in the brain
Gold Biotechnology Inc HEPES Free Acid 2 x 1 kg
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic piperazinic buffer that is useful for a pH range of 6 8 8 2 HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
Biotium Fixation Permeabilization Other Buffers DNA Gel Stains and Accessories DNA Gel Stains Chemical Reagents 50/PK
TAE (Tris-acetate-EDTA) buffer powder packet for preparing 1 L of 1X TAE buffer for nucleic acid gel electrophoresis Box includes 50 packets
Gold Biotechnology Inc MES Free Acid Monohydrate
MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 5 5 6 7 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
Gold Biotechnology Inc MOPS Free Acid Ultra Pure
MOPS is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 6 5 7 9 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
Alkali Scientific CellPro™ PBS Buffer, 1X, p.H 7.2, 1L, 1 Liter
CellPro™ PBS Buffer, 1X pH 7.2, 1 Liter Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
Alkali Scientific QuickSilver™ Buffer Powder, TAE Buffer Powder, 100 pouches
QuickSilverª Buffer Powder Packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, easy to use, and come in a variety of buffers for Nucleic Acid and Protein Electrophoresis, Western Blotting, ELISA, and Cell Culture. Quick and Economical (and low shipping costs!) Convenient premeasured pouches Each pouch makes 1L of 1X buffer No pH adjustment needed Nuclease and protease free Quality and consistency guaranteed Long shelf life Buffers for Electrophoresis of Nucleic Acids and Proteins: TBE, TAE, Fast Running Buffer, Tris-Glycine-SDS, Tris-MOPS-SDS, Tris-MES-SDS Buffers for Western Blotting, ELISA and Cell Culture: TBS, TBST, PBS, PBST
Chem Service Inc Tris(1,3-dichloroisopropyl)phosphate | 13674-87-8 | MFCD00083121 | 250mg
Tris(1,3-dichloroisopropyl)phosphate | MW: 430.88 | 13674-87-8 | MFCD00083121 | 250mg
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS | 6976-37-0 | MFCD00002853 | 500g
BIS-TRIS | Purity: 98% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 500g
Hampton Research HR2-795/1.0 M BIS-TRIS propane pH 7.0 - 100 ml
HR2-795/1.0 M BIS-TRIS propane pH 7.0 - 100 ml. Please note items from this supplier are non-returnable.