Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC 20 mM HEPES, 150 mM NaCl, 240 mM sucrose, pH 7.2 buffer | 30 ML
This physiological buffer is composed of 20 mM HEPES, 150 mM NaCl, and 240 mM Sucrose, adjusted to pH 7.2. It is primarily utilized for resuspending human erythrocyte ghost preparations and acts as the final resuspension medium for purified human right-side-out and inside-out erythrocyte ghosts.
- Composed of 20 mM HEPES, 150 mM NaCl, and 240 mM sucrose
- Adjusted to pH 7.2
- Used for resuspending human erythrocyte ghost preparations
- Serves as final resuspension medium for purified human right-side-out and inside-out erythrocyte ghosts
- Liquid appearance
- Colorless to light yellow
Medchemexpress LLC 20 mM HEPES, 150 mM NaCl, 240 mM Sucrose, pH 7.2 buffer | 10 ML
This physiological buffer is composed of 20 mM HEPES, 150 mM NaCl, and 240 mM Sucrose, meticulously adjusted to a pH of 7.2. It is specifically designed for the resuspension of human erythrocyte ghost preparations.
- Physiological buffer
- Composed of 20 mM HEPES, 150 mM NaCl, and 240 mM Sucrose
- Adjusted to pH 7.2
- Used for resuspending human erythrocyte ghost preparations
AG Scientific Inc HEPES Free Acid 1 KG
HEPES is a zwitterionic N-substituted aminosulfornic acid buffer used in cell culture and as an ampholytic separator to create a pH gradient in isoelectric focusing. It is used as a non-coordinating buffer in solutions with metal ions because it is not likely to form a complex with metal ions. The buffer is able to form free radicals so it is not suitable for redox reactions. It is reported that oxidative DNA damage can be induced by free radicals generated from HEPES buffer in the presence of Au(III).In addition, it is demonstrated that HEPES in RPMI medium potently increases hydrogen peroxide and cytotoxic products during exposure to medium to visible light.
Medchemexpress LLC Mes (2-morpholinoethanesulphonic acid) | 4432-31-9 | 99.85% | 1 ML
MES (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer designed for various biochemistry and molecular biology applications. It functions effectively within a pH range of 5.5-7.0 and is widely recognized as a Good's buffer, suitable for experiments including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Buffer capacity from pH 5.5-7.0
- Ideal for cell culture, enzyme activity determination, electrophoresis, and protein studies
- Solid, white to off-white appearance
- Soluble in water and DMSO
Medchemexpress LLC Heppso | 68399-78-0 | 99.89% | 100 G
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It exhibits a relatively high ability to bind copper(II) and possesses a pKa of 7.84 at a 2.0 mM buffer concentration. It is intended for research use only and is not sold to patients.
- Working pH range of 7.1-8.5.
- Exhibits a relatively high ability to bind copper(II).
- Possesses a pKa of 7.84 at a 2.0 mM buffer concentration.
- For research use only.
- Molecular weight: 268.33.
- Formula: C9H20N2O5S.
- Appearance: Solid.
- Color: White to off-white.
Medchemexpress LLC Ada disodium | 41689-31-0 | 98.0% | 10 G
ADA (disodium) is a buffer salt derived from ADA (adenosine deaminase) and is widely used in biochemical research, especially in the preparation of enzyme buffers. It is for research use only.
- Buffer salt derived from adenosine deaminase
- Widely used in biochemical research
- Used in the preparation of enzyme buffers
Medchemexpress LLC 1-Piperazinepropanesulfonic acid, β-hydroxy-4-(2-hydroxyethyl)- | 68399-78-0 | 99.9% | 10 G
HEPPSO is a zwitterionic buffer. It has a working pH range of 7.1-8.5. HEPPSO demonstrates a relatively high capacity to bind copper(II) and has a pKa of 7.84 at a 2.0 mM buffer concentration.
- Zwitterionic buffer
- Working pH range of 7.1-8.5
- High capacity to bind copper(II)
- pKa of 7.84 at 2.0 mM buffer concentration
Medchemexpress LLC MOPS (sodium salt) | 71119-22-7 | 99.8% | 1 KG
MOPS sodium salt is a buffering agent used in biology. It maintains the pH of mammalian cell culture media and significantly improves the thermal stability of BSA.
- Buffering agent for biological applications.
- Maintains pH in mammalian cell culture media.
- Improves thermal stability of BSA.
Medchemexpress LLC 1-Propanesulfonic acid, 3-(bis(2-hydroxyethyl)amino)-2-hydroxy- | 68399-80-4 | MFCD00038353 | 98.0% | 100 G
DIPSO is a biological zwitterionic buffer with a useful pH range of 7.0 to 8.2. It can interfere with meiotic regulation in mouse oocytes and exhibits surfactant activity at 10 mM. This product is for research use only.
- Useful pH range of 7.0 to 8.2
- Can interfere with meiotic regulation in mouse oocytes
- Exhibits surfactant activity at 10 mM
Medchemexpress LLC Tapso | 68399-81-5 | 98.0% | 100 G
TAPSO is a widely used biological buffer and pH stabilization reagent. It offers an effective pH range between 7 and 8, with pKa values typically falling between 7.5 and 9.0. This compound contains Tris groups and has been observed to exhibit reactive activity with zwitterionic glycine peptides. It is primarily intended for research applications.
- Effective pH range of 7-8.
- pKa values ranging from 7.5-9.0.
- Contains Tris groups.
- Shows reactive activity with zwitterionic glycine peptides.
- Suitable for various research applications.
Medchemexpress LLC N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid | 10191-18-1 | 98.0% | 500 G
BES is a secondary standard biochemical buffer used in diagnostic testing. It offers a stable pH environment for various applications.
- Effective pH range of 6.4 to 7.8
- pKa of 7.15 at 20 °C
- Suitable for diagnostic testing areas
Medchemexpress LLC HEPPS | 16052-06-5 | 99.0% | 50 G
HEPPS (EPPS) is a buffering agent with a useful pH range from 7.3 to 8.7. It reduces Aβ-aggregate-induced memory deficits and rescues cognitive deficits in mice. EPPS is orally active and penetrates the blood-brain barrier. It binds to amyloid-β aggregates and converts them into monomers, thereby reducing the formation of amyloid-β oligomers and plaques. This agent also rescues cell damage induced by amyloid-β aggregates.
- Binds to amyloid-β aggregates and converts them into monomers
- Reduces formation of amyloid-β oligomers and plaques
- Rescues cell damage induced by amyloid-β aggregates
- Improves cognitive dysfunction in APP/PS1 transgenic mice
- Reduces Aβ oligomer and plaque deposition in the brain
- Reduces glial GABA release in the brain
Medchemexpress LLC BES sodium | 66992-27-6 | 97.0% | 25 G
BES sodium is a secondary standard biochemical buffer with an effective pH range of 6.4 to 7.8 (pKa: 7.15 at 20 °C). It is used in the diagnostic testing area.
- Biochemical buffer
- Effective pH range: 6.4 to 7.8 (pKa: 7.15 at 20 °C)
- Used in diagnostic testing
- Appearance: Solid
- Color: White to off-white
- Target: Biochemical assay reagents
- Pathway: Others
Medchemexpress LLC HEPPSO | 68399-78-0 | 99.9% | 25 G
HEPPSO is a zwitterionic buffer designed for various research applications. It operates within a working pH range of 7.1-8.5 and exhibits a relatively high ability to bind copper(II). With a pKa of 7.84 at a 2.0 mM buffer concentration, it is suitable for use in laboratory settings.
- Zwitterionic buffer
- Working pH range of 7.1-8.5
- High ability to bind copper(II)
- pKa of 7.84 at 2.0 mM buffer concentration
- For research use only
Medchemexpress LLC Mops | 1132-61-2 | 99.95% | 25 G
MOPS is a buffering agent used in biology. It is known for its ability to maintain the pH of mammalian cell culture media and significantly improve the thermal stability of BSA.
- Buffering agent for biological applications.
- Maintains pH of mammalian cell culture media.
- Improves thermal stability of BSA.