Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 5 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659782.jpg-250.jpg)
Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 5 Kg
PIPES [Piperazine N N' bis(2 ethanesulfonic acid)] is a biochemical buffer available in USP-grade, designed for a wide range of scientific and research applications. This 5kg container is ideal for laboratories that require large volumes of buffer solution for high-throughput processes, including protein studies, enzymatic assays, and cell culture applications. PIPES is known for its ability to maintain a stable pH under various conditions where other buffers may not be suitable. It is especially effective in high-salt and high-temperature environments. The 5kg pack ensures that large-scale laboratory projects can proceed without the need for frequent reordering, making it both a cost-effective and convenient solution.
![Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659565.jpg-250.jpg)
Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 100 G
PIPES [Piperazine N N' bis(2 ethanesulfonic acid)] is a biochemical reagent supplied in USP grade, specifically formulated for use in biological and chemical research. This 100g bottle is ideal for preparing solutions where pH stability is required. PIPES is commonly used as a buffer in a wide range of biochemical experiments due to its ability to maintain a stable pH, especially under conditions where other buffers may fail. It is used in protein studies, enzyme kinetics, and various biochemical assays. The buffer provides effective pH control in the physiological range, making it a versatile tool in molecular biology, immunology, and other laboratory disciplines.
Sigma Aldrich Fine Chemicals Biosciences POPSO hydrate >=99% (titration) | 68189-43-5 (anhydrous) | 100G
POPSO hydrate >=99% (titration) | Purity: >=99% (titration) | 68189-43-5 (anhydrous) | 100G
AG Scientific Inc MOPS Free Acid 1 KG
MOPS, also known as 3-(N-morpholino)propanesulfonic acid, is one of Good’s buffers. It is N-substituted amino sulfonic acid with a morpholinic ring and has zwitterionic and inert properties. MOPS buffer is broadly used in biological and biochemical studies. It can be mixed with MOPS sodium salt to attain desired pH.MOPS is recommended as an agent for non-coordinating buffers in solutions with metal ions because it hardly forms a complex with most metal ions such as Cu, Ni, Mn, Zn and Co ions. The Good's buffer interferes with the Folin protein assay and partially decomposes when autoclaved in the presence of glucose. MOPS can be used as a Good’s buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water.
Medchemexpress LLC 2-Morpholinoethanesulphonic acid potassium | 39946-25-3 | 99.9% | 50 G
MES potassium is an amphoteric ion buffer widely used in biochemistry and molecular biology experiments. It provides effective buffering capacity around pH 6.15 and is suitable for cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Features a buffer capacity ranging from pH 5.5-7.0
- Functions as a zwitterionic buffer
- Exhibits good buffering properties in biological experiments
- Shows poor affinity with metal ions, suitable for metal ion studies
Medchemexpress LLC Rasagiline-13C3 mesylate | 1391052-18-8 | 270.32 | 1 MG
Rasagiline-13C3 mesylate is a 13C-labeled version of Rasagiline (mesylate). It acts as a highly potent, selective, and irreversible mitochondrial monoamine oxidase (MAO) inhibitor.
- 13C-labeled version of Rasagiline (mesylate)
- Highly potent MAO inhibitor
- Selective MAO inhibitor
- Irreversible mitochondrial MAO inhibitor
- IC50 values of 4.43 nM for rat brain MAO B and 412 nM for MAO A activity
Medchemexpress LLC Rasagiline-13C3 mesylate | 1391052-18-8 | 270.32 | 1 MG
Rasagiline-13C3 mesylate is a deuterium-labeled Rasagiline mesylate, which acts as a highly potent, selective, and irreversible mitochondrial monoamine oxidase (MAO) inhibitor. It exhibits IC50s of 4.43 nM and 412 nM for rat brain MAO B and A activity, respectively.
- Used as a tracer.
- Internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
- Incorporates stable heavy isotopes into drug molecules for quantitation.
- Deuteration can affect the pharmacokinetic and metabolic profiles of drugs.
Medchemexpress LLC AR-13324 analog (mesylate) | 2095432-73-6 | 98.0% | 631.72 | 10 MM * 1 ML
AR-13324 analog mesylate is an analog of AR-13324. AR-13324 is an ROCK and norepinephrine transporter (NET) inhibitor.
- 10 mM * 1 mL in DMSO (ready for reconstitution)
- 10 mM * 1 mL in DMSO
- 5 mg
- 10 mg
- 50 mg
- 100 mg
- 200 mg
![Alkali Scientific MOPSO [3 (N Morpholino) 2 hydroxypropanesulfonic acid], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661261.jpg-250.jpg)
Alkali Scientific MOPSO [3 (N Morpholino) 2 hydroxypropanesulfonic acid], 25 G
MOPSO (3-(N-Morpholino)2-hydroxypropanesulfonic acid) sodium salt is a pH buffering reagent used in biochemical and biological applications requiring stable pH control. This 25g volume of MOPSO sodium salt is ideal for laboratories with specific buffer preparation needs for enzyme assays, protein studies, and other research applications. MOPSO is widely used in molecular biology protocols, including nucleic acid analysis, where maintaining a constant pH of 7.0–7.5 is critical. Its buffering capacity helps maintain pH stability during reactions and experiments, ensuring optimal conditions for enzymatic activity and other biochemical processes. MOPSO is a valuable reagent in research involving protein, DNA, and RNA studies.