Biotin and derivatives
Apexbio Technology LLC Sulfo-NHS-Biotin Kit (90-180 kDa) 3samples
Sulfo-NHS-Biotin labeling is widely utilized in biochemical research to covalently conjugate biotin molecules to free amino groups on protein molecules primarily lysine residues and N-terminal amines The reaction proceeds via activated ester intermediates forming stable amide bonds that facilitate subsequent detection or purification using streptavidin-biotin affinity interactions This labeling method is routinely applied to diverse protein samples particularly immunoglobulin G and other antibodies (molecular weight 90-180 kDa) enabling downstream applications such as immunoassays Western blotting immunoprecipitation and flow cytometry analyses Commonly the labeled proteins are purified rapidly by spin-based filtration to eliminate unreacted small molecules improving purity and yield
Apexbio Technology LLC Sulfo-NHS-Biotin Kit (30-90 kDa) 3samples
Sulfo-NHS-Biotin Kit (30-90 kDa) is a biotinylation reagent set designed to introduce biotin molecules onto primary amines in proteins such as immunoglobulin G (IgG) The biotin molecules in this kit are activated ester derivatives (Sulfo-NHS-biotin) functioning through covalent conjugation reactions where the NHS ester group selectively reacts with primary amino groups (e g lysine residues and protein N-termini) on proteins After conjugation labeled proteins can be applied in biotin-streptavidin platforms for detection immunoassays protein immobilization and affinity purification This kit is optimized for proteins within the molecular weight range of 30-90 kDa accommodating 50-200 g protein per reaction and allows removal of excess biotin or interfering low-molecular-weight components using filtration-based purification for improved labeling quality and purity
Medchemexpress LLC Biotin LC hydrazide (biotinamidocaproyl hydrazide) | 109276-34-8 | MFCD00077659 | 99.8% | 371.50 g/mol | C16H29N5O3S | 100 MG
Biotin LC hydrazide is a long-chain biotinylation reagent that reacts with periodate-oxidized glycoproteins and glycans to introduce biotin labels for detection, affinity capture, and conjugation workflows. It is carbohydrate-reactive and reported to be more sensitive than simple biotin hydrazide, improving labeling efficiency in biochemical assays.
- Long-chain hydrazide reagent for labeling oxidized carbohydrates.
- Reacts with periodate-oxidized glycoproteins and glycans.
- Higher sensitivity than biotin hydrazide for improved detection.
- Soluble in DMSO for solution preparation.
- Suitable for biotinylation of proteins, glycans, and nucleic acids.
Medchemexpress LLC Biotin-PEG3-bromide | 1041766-91-9 | 95.0% | 482.43 g/mol | C18H32BrN3O5S | 100 MG
Biotin-PEG3-bromide is a biotinylated PEG3 linker terminating in a reactive bromide. It provides a short, hydrophilic spacer for biotin conjugation and affinity capture, while the terminal bromide can be displaced by nucleophiles or used in cross-coupling reactions to install diverse handles. The reagent is intended for pull-down assays, streptavidin-mediated capture, and small-molecule bioconjugation workflows.
Medchemexpress LLC PD-L1 Mouse Biotin 50ug | 50ug
PD-L1 protein serves as a ligand for the inhibitory receptor PDCD1/PD-1 and is critical for maintaining immune tolerance This interaction modulates T cell activation limits effector responses and may stimulate IL10-producing T cell subsets PD-L1 Protein Mouse (Biotinylated HEK293 His) is the recombinant mouse-derived PD-L1 protein expressed by HEK293 with C-His labeled tag
Medchemexpress LLC PD-L1 Mouse Biotin 20ug | 20ug
PD-L1 protein serves as a ligand for the inhibitory receptor PDCD1/PD-1 and is critical for maintaining immune tolerance This interaction modulates T cell activation limits effector responses and may stimulate IL10-producing T cell subsets PD-L1 Protein Mouse (Biotinylated HEK293 His) is the recombinant mouse-derived PD-L1 protein expressed by HEK293 with C-His labeled tag
Medchemexpress LLC Bis-PEG9-NHS ester 5g | 1008402-79-6 | 708.71 | C30H48N2O17 | 5 G
Bis-PEG9-NHS ester is a homobifunctional PEG-based linker bearing two N-hydroxysuccinimide (NHS) ester reactive groups, used to conjugate primary amines on proteins, peptides, and small molecules for research applications.
- Homobifunctional PEG9 spacer with two NHS ester reactive groups.
- Facilitates amine-directed bioconjugation to proteins and peptides.
- Hydrophilic PEG spacer improves solubility in aqueous media.
- Suitable for PROTAC and ADC linker chemistry in research.
- High purity for reliable experimental performance.
Medchemexpress LLC Bis-PEG9-NHS ester 250mg | 1008402-79-6 | 250 MG
Bis-PEG9-NHS ester is a homobifunctional polyethylene glycol (PEG) linker bearing two N-hydroxysuccinimide (NHS) ester reactive groups, used for amine-directed bioconjugation in the synthesis of PROTACs and antibody-drug conjugates. The PEG9 spacer increases hydrophilicity to improve solubility and reduce steric hindrance during conjugation.
- Contains two NHS ester reactive groups for amine coupling.
- PEG9 spacer increases hydrophilicity and solubility in aqueous media.
- High purity (≥98.0%) suitable for research and conjugation chemistry.
- Molecular weight 708.71 g/mol and chemical formula C30H48N2O17.
- Viscous, colorless to light yellow appearance for easy handling.
- Applicable to protein labeling, bioconjugation, and linker synthesis.
Apexbio Technology LLC Phos binding reagent (Phosbind) Biotin LC 50mg
Phosbind Biotin LC is a biotin-labeled dinuclear Zn(II) complex that selectively binds phosphorylated molecules at neutral pH (Kd 25 nM to phenyl phosphate) It is widely used in phosphoproteomics workflows to detect phosphoproteins on PVDF membranes via chemiluminescence (with streptavidin-HRP) or enrich phosphorylated peptides/proteins using streptavidin-agarose columns Compared to standard prestained protein markers (e g F4005 with 11 bands from 10 180 kDa) Phosbind enables phospho-specific detection Compatible buffers 20 mM Tris 150 mM NaCl pH 7 5
Medchemexpress LLC PC azido-PEG3-NHS carbonate ester | 1802907-96-5 | MFCD29052189 | 98.7% | 640.60 g·mol⁻¹ | C26H36N6O13 | 5 MG
PC Azido-PEG3-NHS carbonate ester is a photocleavable, heterobifunctional linker designed for covalent labeling of primary amines and for introducing an azide handle for click chemistry. The reagent couples to amine groups via an NHS carbonate, and the photocleavable moiety enables light-triggered release of the attached cargo; supplied as a dry research reagent with recommended cold storage.
Medchemexpress LLC LAIR1 Human Biotin 20ug
LAIR1 protein has a negative regulatory effect on NK cells B cells and T cells It activates the phosphatases PTPN6 and PTPN11 inhibits calcium increases in B cells and regulates cytokine production in CD4 T cells LAIR1 Protein Human (Biotinylated HEK293 Avi-His) is the recombinant human-derived LAIR1 protein expressed by HEK293 with C-Avi C-6 His labeled tag