Dithiothreitol (DTT), also known as Cleland's Reagent, is a powerful reducing agent used to stabilize proteins and enzymes in biochemical research. This 1g reagent is commonly employed in protein purification, enzymatic assays, and structural studies to prevent oxidation of cysteine residues in proteins. DTT works by cleaving disulfide bonds, allowing proteins to maintain their reduced form, which is essential for proper folding and activity. It is widely used in cell lysis, protein extraction, and the stabilization of protein complexes during chromatography and electrophoresis experiments. DTT is crucial for ensuring protein integrity and activity during biochemical analyses in molecular biology and biochemistry.