HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.

16 – 30 of 27,366 results

MilliporeSigma™ Supelco™ PTFE Fitting, Non-Jacketed Liquid Chromatography Column, 30 cm

Constructed of borosilicate glass and are rated at a maximum pressure of 15 psi (1 bar)

Pricing & Availability
Specifications

Length (Metric)	30 cm
Column Type	Chromatography
Material	Borosilicate Glass (Column), PTFE (Fittings)

Thermo Scientific™ Acclaim™ HILIC-10 HPLC Columns

Use this HILIC column to retain and separate highly polar molecules that are unretained by reversed-phase chromatography.

Pricing & Availability
Specifications

Pore Size	120 Å
Carbon Load	8%
Stationary Phase	Proprietary
Temperature	70°C
Phase	HILIC
For Use With	LC/MS
Endcapped	Yes
Column Type	HILIC
pH	2 to 8
Surface Area	300 m²/g
Product Line	Acclaim

Thermo Scientific™ Syncronis™ C18 HPLC Columns

Robust separations with highly pure, high surface area silica that is double endcapped Syncronis C18 preparative HPLC columns with inertness toward basic compounds.

Pricing & Availability
Specifications

Pore Size	100 Å
Particle Size	5 μm
Carbon Load	16%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Spherical, Fully Porous, Ultrapure Silica
Column Type	Reversed Phase
pH	2 to 9
Surface Area	320 m²/g
USP Type	L1

MilliporeSigma™ Supelco™ BIOshell™ A400 Protein C4 HPLC Column, 400 Å, 3.4 μm Particle Size

BIOshell™ 400 Å Protein C4 columns contain 3.4 μm advanced Fused-Core particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins.

Pricing & Availability
Specifications

Pore Size	400 Å
Particle Size	3.4 μm
Carbon Load	0.4%
Stationary Phase	C4 (Butyl)
Phase	Reversed Phase
Matrix	C4 (Butyl) Phase/Fused-Core Particle Platform
Endcapped	Yes
pH	2 to 9
Material	Stainless Steel Hardware
Technique	Fused-Core HPLC
Surface Area	15 m2/g
Product Line	BIOshell

Thermo Scientific™ Acclaim™ 300 C18 HPLC Columns

Use these wide-pore C18 columns to achieve high-resolution protein separations.

Pricing & Availability
Specifications

Pore Size	300 Å
Stationary Phase	C18
Temperature	60°C
For Use With	LC/MS
Endcapped	Yes
Column Type	Reversed Phase
Green Features	FSC
Surface Area	100 m²/g
Particle Size	3 μm
Flow Rate	1 mL/min
Carbon Load	8%
pH	2.5 to 7.5
Product Line	Acclaim
USP Type	L1

MilliporeSigma™ Chromolith™ WP 300 Protein A HPLC Column

Chromolith™ WP 300 columns allow flow rate flexibility for much higher throughput and more solvent options for optimum selectivity.

Pricing & Availability
Specifications

Pore Size	Monolith
Stationary Phase	Protein A bonding phase
Temperature	2 to 45°C
Phase	Affinity Separation
Matrix	Monolithic silica
Length (Metric)	25 mm
Endcapped	No
pH	1.5 to 7.5
Material	PEEK
Technique	HPLC, LC/MS
Surface Area	120 m²/g
Max. Pressure	200 bar

MilliporeSigma™ Supelco™ Discovery™ C8 HPLC Column, 5 μm Particle Size

Discovery™ C8 HPLC column has been used in the separation of fluoroquinolone antibiotics in tablets using reversed-phase high-performance liquid chromatography (RP-HPLC).

Pricing & Availability
Specifications

Pore Size	180 Å
Particle Size	5 μm
Stationary Phase	C8 (Octyl)
Phase	Reversed Phase
Matrix	C8 (Octyl) Phase/Silica Gel, High Purity, Spherical Base Material
Endcapped	Yes
pH	2 to 8
Surface Area	200 m2/g
Product Line	Discovery

Thermo Scientific™ Hypersil GOLD™ PREP C18 HPLC Columns

Easily scale up your method to prep LC using Hypersil GOLD C18 preparative HPLC columns for excellent peak shape in a variety of formats including XtendedLife technology.

Pricing & Availability
Specifications

Pore Size	175 Å
Carbon Load	10%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Spherical, Fully Porous, Ultrapure Silica
Column Type	Reversed Phase
pH	2 to 9
Surface Area	220 m²/g
USP Type	L1

MilliporeSigma™ Supelco™ Kromasil C8 HPLC Column, 100 Å, 10 μm Particle SIze

For small molecules and peptides.

Pricing & Availability
Specifications

Pore Size	100 Å
Particle Size	10 μm
Stationary Phase	C8 (Octyl)
Phase	Reversed Phase
Matrix	C8 (Octyl) Phase
Product Line	Kromasil

MilliporeSigma™ Supelco™ Omnifit™ EZ Benchmark Columns with 1 Adjustable and 1 Fixed Endpiece

Omnifit™ Labware complete columns are supplied assembled and ready for use, with tubing and fittings to connect the column into an existing system.

Pricing & Availability
Specifications

Column Type	Chromatography
Product Line	Omnifit

Thermo Scientific™ ProPac™ WCX-10 HPLC Columns

Separate proteins and their variants using these weak cation-exchange columns with exceptionally high resolution.

Pricing & Availability
Specifications

Pore Size	Nonporous
Particle Size	10 μm
Stationary Phase	Carboxylate
Temperature	60°C
Phase	Ion Exchange
Column Type	Ion Exchange
pH	2 to 12
Quantity	Each
Product Line	ProPac

Thermo Scientific™ DNAPac™ RP Columns

Get high resolution LC and LC-MS separation of oligonucleotides, including double-stranded (ds) DNA/RNA fragments, using these DNAPac reversed phase columns.

Pricing & Availability

Thermo Scientific™ PepMap™ Neo Trap Cartridge

Use the Thermo Scientific™ PepMap™ Neo Trap Cartridge with PepMap™ Neo UHPLC Columns to filter impurities from your peptide mapping samples.

Pricing & Availability

Thermo Scientific™ ProSwift™ RP-1S PSDVB Reversed Phase HPLC Column, Monolith, 4.6 mm x 50 mm

Column Format: Analytical Column; Pore Size: Monolith; Column Phase: Reversed Phase

Pricing & Availability

Thermo Scientific™ Accucore™ C30 HPLC Columns

Use this specially designed solid core column for fast, high-resolution separations of hydrophobic, long-chain compounds.

Pricing & Availability
Specifications

Pore Size	150 Å
Particle Size	2.6 μm
Carbon Load	5%
Stationary Phase	C30
Temperature	70°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Ultrapure Silica
pH	2 to 8
Surface Area	80 m²/g
Max. Pressure	14500 psi (1000 bar)
Product Line	Accucore
USP Type	L62

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HPLC Columns

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Thermo Scientific™ Acclaim™ HILIC-10 HPLC Columns Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Thermo Scientific™ Acclaim™ 300 C18 HPLC Columns Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Thermo Scientific™ ProPac™ WCX-10 HPLC Columns GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Thermo Scientific™ DNAPac™ RP Columns Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Thermo Scientific™ ProSwift™ RP-1S PSDVB Reversed Phase HPLC Column, Monolith, 4.6 mm x 50 mm Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Thermo Scientific™ Accucore™ C30 HPLC Columns GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More