HPLC Columns
Imtakt SS-C18 SCHERZO 4.6X150MM 3UM
The Scherzo family of columns is one of the most unique and versatile series of columns on the market today. These multi-mode columns utilize reversed phase, cation and anion exchange, as well as normal phase modes all on the same column, virtually eliminating the need for ion-pairing reagents. -Polar and non-polar retention in Reversed Phase conditions Multi-level retention without ion-pairing reagents- Compatible with LC-MS- Most unique selectivity of any HPLC column on the market today- Wide range of method development possibilities
Chiral Technologies, Inc. CHIRALPAK AD-H SFC 4.6X100MM
Coated Polysaccharide, CHIRALPAK AD-H, 5 micron,4.6 mm ID x 100mm LN, SFC analytical column
Waters Corp XBridge Preparative Column, BEH C18, 10 x 150 mm, 10 μm
These OBD Preparative Columns feature uniform, optimum packed bed density, enabling direct scalability from analytical to preparative chromatography. They offer excellent column lifetime and consistent performance, resulting in superior resolution, peak shape, and mass loading.
- Uniform packed bed density.
- Scalable from analytical to preparative chromatography.
- Excellent column lifetime and consistent performance.
- Delivers superior resolution, peak shape, and mass loading.
- BEH Technology™ for broad compound compatibility.
- Exceptional peak shape for basic analytes at high pH.
- Wide pH stability (1 to 12).
- High temperature stability (up to 80 °C low pH, 60 °C high pH).
Waters Corp BioResolve RP mAb Polyphenyl, 450Å, 2.7 μm, 3 x 50 mm
This BioResolve RP mAb Polyphenyl Column is for reverse phase protein separation and analysis. It uses 2.7 μm superficially-porous-silica particles with a 450Å pore diameter, functionalized for LC or LC-MS analyses of intact IgG monoclonal antibodies and their fragments.
- Optimized for reverse phase protein separation and analysis.
- Polyphenyl bonding for LC/LC-MS of intact IgG monoclonal antibodies and fragments.
- Manages biotherapeutic drug analysis challenges with high throughput and sensitivity.
- VanGuard column protection enhances lifetime and performance.
- Addresses challenges in intact protein analysis of monoclonal antibodies and antibody drug conjugates.
- Works with UHPLC, UPLC, and HPLC systems, supporting higher flow rates and back pressures.
- Offers a wide and usable pH range.
- Reduces injection-to-injection carryover.
Waters Corp HPLC Column, BEH C18 AX, 2.5 μm, 2.1 x 100 mm, VanGuard FIT
These columns provide excellent retention for polar acidic analytes under reversed-phase conditions. Their reversed-phase/anion-exchange mixed-mode chemistry is stable from pH 2-10, offering complementary selectivity to traditional C18 stationary phases. The VanGuard FIT Column format is an all-in-one, guard-column design engineered to reduce dead volume and increase column lifetime.
- Separates polar acidic analytes via HPLC.
- Anion-exchange chemistry supports analytes from 2 to 10 pH.
- VanGuard FIT Technology prevents signal loss.
- Reduces dead volume, increases efficiency.
- Offers complementary selectivity to C18 phases.
- Maximizes throughput for reliable data.
- Effective for amphiphilic compound separations.
- Stable BEH particle morphology resists acids and bases.
- Compatible with 100% aqueous mobile phases.
Waters Corp Atlantis Column, BEH C18 AX, 1.7 μm, 2.1 x 30 mm
The Atlantis Premier BEH C18 AX Columns offer excellent retention for polar acidic analytes under reversed-phase conditions. Its reversed-phase/anion-exchange mixed mode chemistry is stable from pH 2 to 10 and provides complementary selectivity to traditional C18 stationary phases, aiding method development.
- Excellent retention for polar acidic analytes under reversed phase conditions.
- Reversed-phase/anion-exchange mixed mode chemistry stable from pH 2 to 10.
- Complementary selectivity to traditional C18 stationary phases for method development.
- Hybrid particle substrate with spherical shape.
- Maximum pressure of 18000 psi (1240 Bar).
- 1.7 μm particle size; 2.1 mm inner diameter; 30 mm length.
- 95 Å pore size.
- UPLC and UHPLC system compatible.
- Endcapped with low silanol activity.
- 17% carbon load.
- eCord enabled.
Waters Corp Column, BioResolve SCX mAb, 3 μm, 2.1 mm ID, 50 mm Length, VanGuard FIT
These corrosion-resistant columns contain non-porous, polymer-based particles grafted with a rigorously-optimized, multi-component network of negatively charged sulfonic acid ligands.
- Utilizes an optimized network of negatively charged sulfonic acid ligands for charge variant analysis of monoclonal antibodies and other proteins.
- Based on non-porous particle technology for optimal protein diffusion kinetics and pressure tolerances across UHPLC, HPLC and UPLC systems.
- Provides high resolution and robust separations quickly and efficiently for method development.
- The included cartridge actively removes contaminants from the mobile phase stream.
- Cartridge upholds mechanical and chemical stability, offering the same pH range and sorbent as the column.
Waters Corp CORTECS HILIC Column, 90Å, 2.7 μm, 2.1 mm x 50 mm
This column is a CORTECS HILIC column designed for the retention of very polar compounds using hydrophilic-interaction chromatography (HILIC). It offers an orthogonal separation mode to reversed-phase for mixtures of ionizable and very polar compounds, leveraging solid-core particle technology.
- Enables seamless method transfer between UPLC, UHPLC, and HPLC platforms.
- Exhibits maximum chromatographic performance.
- Retains very polar bases, while acidic, neutral, and non-polar compounds will have limited retention.
- Combination of acetonitrile-rich mobile phase and polar, unbonded stationary phase provides increased mass spectrometry response.
- Allows direct injection of organic extracts from common sample preparation techniques such as solid-phase extraction, liquid-liquid extraction, and protein precipitation.
Waters Corp XSelect Premier Peptide CSH C18 Column, 130 Å, 2.5 μm, 2.1 x 100 mm
This column is specifically designed for peptide separations, featuring a low and carefully-defined concentration of positive charges that ensures excellent peak shape. It is ideal for LC and LC-MS applications due to minimal dependence on strong ion pairing agents. The 2.1 mm ID columns are recommended for low dispersion systems and are part of the MaxPeak Premier Class, delivering enhanced reproducibility, peak shape, and recovery for metal-sensitive analytes.
- First choice for peptide separations.
- Ensures excellent peak shape for peptides using FA- or TFA-containing mobile phases.
- Quality controlled with cytochrome c digest standards.
- Unique design includes an integrated, replaceable 5 mm pre-column to extend analytical column life.
- VanGuard FIT cartridge replacements use the same qualified sorbent.
- Suggested for peptide separations, analysis, mapping, and synthetic peptides.
Waters Corp CORTECS C18 Column, 1.6 μm, 90 Å, 2.1 x 75 mm
CORTECS C18 Columns are general purpose, high-efficiency columns based on a solid-core particle that offer balanced retention of acids, bases, and neutrals at low and mid-range pH.
- Chemistry: C18
- Separation mode: Reversed Phase
- Particle substrate: Silica
- pH range: 2 to 8
- Temperature limits: 45°C
- Maximum pressure: 18000 psi (1240 Bar)
- Endcapped: Yes
- Silanol activity: Low
- Particle shape: Spherical
- Particle size: 1.6 μm
- Endfitting type: Parker-style
- Pore size: 90 Å
- Format: Column
- Surface area: 100
- System compatibility: UPLC, UHPLC
- Particle technology: Solid Core
- USP classification: L1
- Inner diameter: 2.1 mm
- Length: 75 mm
- Carbon load: 6.6%
- eCord: Yes
- Units per package: 3
Waters Corp ACQUITY Premier HSS T3 Column, 1.8 μm, 2.1 x 50 mm
This column features a universal, silica-based bonded phase compatible with 100% aqueous mobile phase, suitable for developing separations for polar and non-polar compounds. It offers ultra-low MS bleed and is fully scalable to HPLC. MaxPeak High Performance Surface hardware reduces unwanted analyte/surface interactions, preventing poor peak shape and signal losses. The VanGuard FIT Column design protects the column from contaminants, extending its lifetime.
- Maximizes UPLC column efficiency, aids MS adoption.
- High-Strength Silica (HSS) particle technology increases retentivity of polar and non-polar compounds.
- Provides increased mechanical stability under higher backpressures.
- VanGuard FIT column protection prevents signal intensity losses from fouling.
Waters Corp SEC Column, 250 Å, 2.5 μm, 7.8 mm ID, 300 mm Length, with MaxPeak Premier Protein SEC Guard
This SEC column provides reliable protein size variant separations by combining MaxPeak Premier High Performance Surfaces and novel BEH-PEO Particle technologies. It minimizes undesired secondary interactions, which reduces method development time and improves data confidence for complex biotherapeutics.
- Achieves reliable protein size variant separations from 10,000 to approximately 650,000 Daltons.
- Minimizes undesired secondary ionic or hydrophobic interactions.
- Reduces method development by enabling a “generic” approach for various samples.
- Increases analyte recovery, sensitivity, and reproducibility with MaxPeak High Performance Surfaces technology.
- Reduces column conditioning and passivation times.
- Allows for simpler mobile phases without complex additives.
- Lowers the cost per analysis by utilizing Premier SEC Guards.
