Protein Method Blocking Buffers
Alkali Scientific CellPro™ BSA-Blocking Buffer, 3% in TBS, 250mL bottle
CellPro™ BSA-Blocking Buffer, 3% in TBS, 250mL bottle
LICOR Intercept (TBS) Blocking Buffer and Diluent Kit
Try Intercept (TBS) Blocking Buffer and Intercept T20 (TBS) Antibody Diluent for improved results in your quantitative Western blots and other immunoassays. Intercept Blocking Buffers provide excellent blocking performance with low background and low variability. Intercept T20 Antibody Diluents improve the specificity of the primary and secondary antibodies, reducing off-target effects. They are preformulated with Tween 20. There’s no need to mix the diluent yourself, which saves you time and reduces potential variation. This kit includes Intercept (TBS) Blocking Buffer (1 x 500 mL) and Intercept T20 (TBS) Antibody Diluent (2 x 500 mL)
LICOR Intercept (PBS) Protein-Free Blocking Buffer (500 mL)
Intercept (PBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. This buffer contains no proteins, for assays where use of animal-based products is prohibited. They are an excellent choice for biotin-streptavidin detection and suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in phosphate-buffered saline and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
Bioworld Blotto (5%) in PBS, 1 L
Blotto in PBS Blot blocking buffer used to block non-specific binding for antibodies on the membrane Technical Information: 5% nonfat dry milk, 0.02% Sodium azide in PBS
Diagnostic Biosystems BACKGROUND BLOCKER Background Blocker (1X)
To block nonspecific background staining observed during immunostaining.\n\nBackground Blocker is unique serum-free blocking solution used to reduce nonspecific background staining. It is a universal blocking agent which eliminates the need to match species with the link antibody. This blocking reagent has been shown to be more superior than serum-based blocking solutions. In addition to immunihistochemistry, Background Blocker can also be used for ELISA and Western Blot techniques.
Electron Microscopy Sciences Aurion Blocking Solution Donkey 30 ML
Aurion has developed Blocking Solutions which effectively block ‘sticky' surfaces by multi-point hydrophobic and charge-based interactions. The Blocking Solutions are specifically designed to meet the characteristics of each type of secondary conjugate. With adequate blocking and incubation media, background reactions are minimized whereas specific reactions are not hampered.
Medchemexpress LLC 1,1'-(4,4'-(((5-chloropyrimidine-2,4-diyl)bis(azanediyl))bis(3-methoxy-4,1-phenylene))bis(piperazine-4,1-diyl))diethanone | 1472795-20-2 | MFCD28133395 | 96.2% | 609.12 g·mol^-1 | C30H37ClN8O4 | 10MM 1ML
KRCA-0008 is a selective dual inhibitor of anaplastic lymphoma kinase (ALK) and activated Cdc42-associated kinase 1 (ACK1/TNK2) with reported IC50 values of approximately 12 nM for ALK and 4 nM for ACK1. It is intended for preclinical research into oncogenic signalling and cancer biology and is supplied as a concentrated DMSO solution suitable for in vitro biochemical and cellular assays.
- Potent dual kinase inhibition with low nanomolar IC50 values.
- Suitable for biochemical and cellular assays.
- Provided as a 10 mM solution in DMSO for convenient dosing.
- High purity (≈96.2%) with stated stability and storage recommendations.
- Intended for research use only; not for human consumption.
Sigma Aldrich Fine Chemicals Biosciences OmniPur(R) Nonfat Powdered Milk | 10GM
OmniPur(R) Nonfat Powdered Milk | 10GM
Electron Microscopy Sciences Aurion Blocking Solution (Protein A & G) 30 ML
Aurion has developed Blocking Solutions which effectively block ‘sticky' surfaces by multi-point hydrophobic and charge-based interactions. The Blocking Solutions are specifically designed to meet the characteristics of each type of secondary conjugate. With adequate blocking and incubation media, background reactions are minimized whereas specific reactions are not hampered.
INNOVEX BIOSCIENCES UNIVERSAL Fc RECEPTORS BLOCKER NON-ANTIBODY BASED 60 ML
Innovex peptide-based Fc Blocker universally blocks all types Fc receptors (I, II, III) of human, mouse & all animal species; Contains NO antibody, Ig or Ig fragments, is used for IHC, IF & flow cytometry.
Rockland Immunochemicals Blocking Buffer for Fluorescent Western Blotting - MB-070S
Western Blot Blocking Buffer is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. This buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. See www.rockland-inc.com for specific protocols. This product is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (20X, with 1% Tween-20, pH 7.4) - 1L
20X Tris Buffered Saline - Tween (TBST) is a buffer-surfactant solution supporting a stable pH range between 7.4 and 8.0. When combined in a variable ratio, TBST is an effective wash buffer in Western blotting, in situ hybridization procedures, and immunohistochemistry staining, as it minimizes non specific binding of the materials without disrupting antigen-antibody binding. TBST is also used as an antibody diluent in immunoassays, and supports an array of molecular biology techniques.
Cell Signaling Technology Animal-Free Blocking Solution(5X) 250 ml
Animal-Free Blocking Solution(5X) 250 ml
Santa Cruz Biotechnology Tris Buffered Saline with Tween 20: 1 L of 20X, 1 L
Tris Buffered Saline with Tween 20: 1 L of 20X is a high-quality 20X concentrate for dilution to a 1X working solution commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. Tris Buffered Saline with Tween 20 (TBST) is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.
Seracare Life Sciences Inc 10X DULBECCO S PBS W/ TWEEN 20
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications as well as tissue and cell culture procedures. This modified Dulbecco's buffer is not formulated with magnesium or calcium salts. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures. pH=7.4. Conductivity controlled.