Protein Method Blocking Buffers
Genetex Inc GeneTex Blocking Buffer, 5% Milk For WB and ELISA, Reduces Background Interference, 250 mL
GeneTex Blocking Buffer, TBS with 5% Milk is a ready-to-use solution that enhances assay sensitivity by minimizing non-specific background interference in immunological detection methods. It is specifically validated for Western blotting (WB) and ELISA, improving signal-to-noise ratios by binding to sites of nonspecific interaction. This liquid formulation comes in a 250 mL volume, making it ideal for various applications including immunohistochemistry.
Abcam Immunoassay Blocking Buffer, 1000ML
Abcam’s Immunoassay Blocking Buffer (ab171534) effectively preserves the conformation and activity of dried proteins in immunoassays.
The product is subject to the following: Abcam Restricted Use Statement
Rockland Immunochemicals Blocking Buffer (2X) for Fluorescent Western Blotting - MB-070-0050
This product is a 2X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 2X concentrate with 1 parts TBS or equivalent. This buffer was meticulously prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.
LICOR TBS Blocking Buffer and PVDF Kits (single buffer kit)
The non-mammalian Intercept Blocker in these kits is in TBS (Tris-buffered saline) containing 0.06% Kathon II. The Immobilon-FL PVDF membrane included in these kits has been tested to ensure a low background for infrared Western blot success. This kit contains Intercept (TBS) Blocking Buffer (500 mL) and Millipore Immobilon-FL PVDF Membrane (1 roll; 0.45µm, 26.5 cm x 3.75 m).
LICOR Intercept T20 Protein-Free Antibody Diluent (10x500mL)
Intercept T20 (TBS) Protein-Free Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Protein-Free Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. This diluent can be used where animal-based products are prohibited. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Protein-Free Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Teknova 1X PBS with 0.1% Tween 80. 1000mL. Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Biotium TrueBlack IF Background Suppressor System (Permeabilizing)
The TrueBlack Background Suppressor System (Permeabilizing) is a buffer system designed for blocking background from both non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections.
LICOR Intercept T20 (PBS) Protein-Free Antibody Diluent (500mL)
Intercept T20 (PBS) Protein-Free Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (PBS) Protein-Free Blocking Buffer preformulated with Tween 20 for use in a phosphate-buffered saline (PBS) system. This diluent can be used where animal-based products are prohibited. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (PBS) Protein-Free Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
ILEX LIFE SCIENCES LLC Block Ace Powder: blocking reagent for immunological experiments (1 x 40 g)
Block Ace Powder: blocking reagent for immunological experiments. Block Ace is a blocking agent prepared from bovine milk proteins (lactoprotein). Since lactoprotein contains a lot of hydrophobic groups, it shows a high affinity to plastic, membrane filter, etc. Block Ace exhibits a superior blocking effect on nonspecific adsorption of antigens or antibodies in ELISA, RIA and immunoblocking methods compared with conventional blocking agents such as BSA (Bovine Serum Albumin). FEATURES: Since Block Ace has high blocking effects and maintains the background at a low level, it is useful for low-concentration measurement such as ELISA. Block Ave is heat-treated and thus bovine-derived antibodies, enzymes and viruses are inactivated. Block Ace is stable for heat and freezing. It can stabilize physiologically active substances and can be used as a diluting agent. Block Ace can be rapidly dissolved in water. MANUFACTURER: KAC Co., Ltd., Kyoto-city 603-8432, Japan.
Bioworld BBS-Casein (10X), 500 mL
BSS-Casein, supplied in 10X PBS and 5% Csein. Technical Information:10X Concentrate has a working pH of 8.5 ( +/- 0.1)Formulated in a borate buffered physiologic saline bufferSold as 2 x 100mL or 500mL.
Vector Laboratories Animal-Free Blocker and Diluent (AFB), Ready-to-Use (R.T.U.) 100 ml
R.T.U. Animal-Free Blocker and Diluent is a ready-to-use antibody diluent and blocking reagent that stabilizes diluted antibody solutions and diminishes background due to non-specific binding in immunohistochemical applications. It contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations where these or other traditional blocking agents are ineffective. It is especially convenient for multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used.
Abcam SN x 482, R-Type Ca2+ (Cav2.3) channel blocker, 10UG
MW 4495 Da, Purity >97%. SNX 482, R-Type Ca2+ (Cav2.3) channel blocker. Achieve your results faster with highly validated, pure and trusted compounds.
The product is subject to the following: Abcam Restricted Use Statement
Crescent Chemical Co Inc Denhardt's Solution, 50X Concentration, 10mL
Denhardt's Solution, 50X Concentration, 10mL, Molecular Biology Grade, DNase/RNase not detected.Suitable for nucleic acid hybridization. Denhardt's solution is a mixture of blocking agents capable of saturating non-specific binding sites and to be used in membrane-based hybridization protocols. It is recommended for use with nylon membranes. Storage Conditions: -15 °C TO -25 °C
Rockland Immunochemicals Blocking Buffer for Fluorescent Western Blotting 10-PACK (10 x 500 ml) - MB-070-010
Western Blot Blocking Buffer is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. This buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. See www.rockland-inc.com for specific protocols. This product is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
Boca Scientific Inc SmartBlock™, 500ml
SmartBlock™ is a BSA-free, peptide-based blocker for saturating free binding sites on plastic surfaces or other protein binding surfaces, removing background and preventing unwanted binding. This reagent was produced by chemically modifying peptides in a specific process that resulted in a peptide solution with strong blocking characteristics. SmartBlock™ has high lot-to-lot consistency that had previously only been obtained by synthetic blockers. For many assays, SmartBlock™ is an economical and reliable alternative to BSA-containing solutions. Problems caused by BSA within blockers, such as BSA-interactions and cross reactivities, can be eliminated by using SmartBlock™.