Protein Method Blocking Buffers
Rockland Immunochemicals Blocking Buffer for Fluorescent Western Blotting 3-PACK (3 x 500 ml) - MB-070-003
Western Blot Blocking Solution is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. Blocking buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. Western Blot blocking buffer is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
Abcam 10 x Blocking Buffer, 50ML
10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding. - Cited in over 30 publications - Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB) - 100% casein blocking as preservative
The product is subject to the following: Abcam Restricted Use Statement
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (20X, with 1% Tween-20, pH 7.4) - 4x1L
20X Tris Buffered Saline - Tween (TBST) is a buffer-surfactant solution supporting a stable pH range between 7.4 and 8.0. When combined in a variable ratio, TBST is an effective wash buffer in Western blotting, in situ hybridization procedures, and immunohistochemistry staining, as it minimizes non specific binding of the materials without disrupting antigen-antibody binding. TBST is also used as an antibody diluent in immunoassays, and supports an array of molecular biology techniques.
Biogenex Laboratories Power Block
Power Block ™ reagent is a highly effective blocking reagent capable of reducing non-specific background staining associated with immunodetection techniques such as immunocytochemistry, ELISA, and Western blotting. Antibodies tend to bind non-specifically to membranes and tissues. To minimize this nonspecific binding, a blocking buffer is used to enhance signal-to noise ratio. Power Block™ reagent shows no cross-reactivity and it blocks more effectively than serum albumins. The blocking potency of Power Block™ reagent is superior to most other conventional formulations in producing a high signal-to-noise ratio. Power Block™ reagent may also be used as effective diluents for antibodies.""""
Vector Laboratories Animal-Free Blocker and Diluent (AFB), Ready-to-Use (R.T.U.) 100 ml
R.T.U. Animal-Free Blocker and Diluent is a ready-to-use antibody diluent and blocking reagent that stabilizes diluted antibody solutions and diminishes background due to non-specific binding in immunohistochemical applications. It contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations where these or other traditional blocking agents are ineffective. It is especially convenient for multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used.
INNOVEX BIOSCIENCES UNIVERSAL Fc RECEPTORS BLOCKER NON-ANTIBODY BASED 5 ML
UNIVERSAL Fc RECEPTORS BLOCKER NON-ANTIBODY BASED 5 ML
Rockland Immunochemicals Blocking Buffer for Fluorescent Western Blotting - MB-070
Western Blot Blocking Solution is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. Blocking buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. Western Blot blocking buffer is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (20X, with 2% Tween-20, pH 7.4) - 1L
20X Tris Buffered Saline - Tween (TBST) is a buffer-surfactant solution supporting a stable pH range between 7.4 and 8.0. When combined in a variable ratio, TBST is an effective wash buffer in Western blotting, in situ hybridization procedures, and immunohistochemistry staining, as it minimizes non specific binding of the materials without disrupting antigen-antibody binding. TBST is also used as an antibody diluent in immunoassays, and supports an array of molecular biology techniques.
Sigma Aldrich Fine Chemicals Biosciences OmniPur(R) Nonfat Powdered Milk | 10GM
OmniPur(R) Nonfat Powdered Milk | 10GM
RIGAKU REAGENTS Block, Wizard pH Buffer, 96 formulations, 1mL volumes
The Wizard pH Buffer screen is an optimization screen that can be applied to your crystallization hit optimization to simultaneously explore the effects of pH and buffer composition on crystal growth. The Wizard pH Buffer screen is a set of 96 unique buffer solutions encompassing 12 unique buffer systems at 8 pH points, incrementally varied by 0.4 pH units.96-well block plate formulations are 1.0 ml each in a 96 deep-well matrix block plate. All formulations are prepared with ASTM type-1 water and high-purity chemicals, 0.2 �m filtered in sterile packaging.
LI COR BIOTECH LLC Intercept (TBS) Protein-Free Blocking Buffer (10x500mL)
Intercept (TBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays where animal-based products are prohibited. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no proteins, for assays where animal-based products are prohibited, it can be used with the widest range of targets for Western blot detection, it is ideal for primary antibodies that are optimized for Tris-based buffer systems, it can be used for detection of phosphorylated targets, it has superior performance for alkaline phosphatase detection, and it is an excellent choice for biotin-streptavidin detection. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in TBS and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.