Protein Method Blocking Buffers
Bioworld NFDM Diluent/Blocking, 1 L
NFDM Diluent/Blocking 4X, in a priority physiological saline borate buffer is used to minimize non-specific binding of antibody Technical Information:pH 7.6Sold as 10 x 100mL.
Medchemexpress LLC (-)-KCC2 blocker 1 | 1228439-73-3 | 98.24% | 415.50 | 1 MG
KCC2 blocker 1 is an orally active and selective K+-Cl- cotransporter KCC2 blocker with an IC50 of 1 μM. It is a benzyl prolinate. The product is for research use only and not sold to patients.
- Appearance: Solid
- Color: Light yellow to khaki
Bioworld BBS-Casein (10X), 200 mL
BSS-Casein, supplied in 10X PBS and 5% Csein. Technical Information:10X Concentrate has a working pH of 8.5 ( +/- 0.1)Formulated in a borate buffered physiologic saline bufferSold as 2 x 100mL or 500mL.
As One International Inc 50x Glass Blocking agent, 3 x 1.25 ml
One extra challenge using the LightCycler instrument is that the PCR reagents can form precipitates on the glass capillary surface as the Real time PCR progresses. To prevent this event AS ONE offers a special reagent for blocking the glass capillaries during the Quantitative PCR reaction. The Glass blocking agent is a 50x solution (1 ul per 50 ul PCR reaction).
LICOR Intercept T20 (PBS) Antibody Diluent (10 x 500 mL)
Intercept T20 (PBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects.The diluent contains Intercept (PBS) Blocking Buffer preformulated with Tween 20 for use in a phosphate-buffered saline (PBS) system. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (PBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
IBI SCIENTIFIC 3P 10X PBST (PBS W/0.5% TWEEN 20) 4/PACK
Sterile buffer solution containing sodium phosphate, sodium chloride, potassium phosphate and potassium chloride with 0.5% TWEEN 20.Sterile filtered using 0.1um filterDNase, RNase and protease free.Sterility validated using USP <71> Test for SterilityDoes not contain calcium, magnesium, phenol red or sodium pyruvate. IBI 10X PBST (10X PBS) with TWEEN 20 (0.5% TWEEN 20) is a concentrated sterile solution in a 1 liter bottle that is ideal for use in immunoassay procedures such as Western Blotting and ELISAs in microtiter plate wells.IBI 10X PBST is mixed and bottled in our liquid production facility here in Dubuque, Iowa. Our high output pure water system produces endotoxin free and nuclease free water at a rate of 100 gallons per/hr. This water is the basis for all of our liquid blending production here at IBI.
Abcam Immunoassay Blocking (BSA Free), 50ML
Abcam’s Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.
The product is subject to the following: Abcam Restricted Use Statement
Electron Microscopy Sciences Aurion Blocking Solution Donkey 30 ML
Aurion has developed Blocking Solutions which effectively block ‘sticky' surfaces by multi-point hydrophobic and charge-based interactions. The Blocking Solutions are specifically designed to meet the characteristics of each type of secondary conjugate. With adequate blocking and incubation media, background reactions are minimized whereas specific reactions are not hampered.
Quality Biological Inc DPBS with TWEEN 20 without Ca & Mg, 1000 mL
DPBS can be used to provide a buffer system to maintain cell culture media in the physiological pH range of 7.0 to 7.6. ¿ DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ DPBS is also used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate, to wash the wells of microtiter plates, and to wash cells during preparation and serial transfer. ¿ 1X working solution is recommended ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Cell Biolabs Inc PhosphoBLOCKER™ Blocking Reagent, 200 g dry blend (4 Liters prepared)
The utilization of anti-phosphoprotein antibodies in western blotting has become a commonly used tool for signal transduction research. Unfortunately, low levels of endogenous phosphoprotein in various cell lysates often can not be detected, even with high concentrations of antibody and long exposure times. Most commercially available western blot blockers (e.g. dry milk, serum) are sufficient to block the unreacted sites on the membrane, reducing the amount of nonspecific antibody binding during the assay; however, they are not designed to preserve phosphoprotein antigens during blotting. Cell Biolabs’ PhosphoBLOCKER™ contains a proprietary formulation that provides several advantages over conventional blockers: (1) It is designed specifically for phosphoprotein blotting; (2) It enhances low level phosphoprotein signal without increasing background; and (3) it is an easy to use premixed dry blend. 200 grams (reconstituted to 4 liters).
Alkali Scientific Blotto Tween 20 Blocking Solution in TBS with Azide, 500 Milliliters
Blotto Tween 20 Blocking Solution in TBS with Azide is a pre-prepared blocking reagent commonly used in immunoassays such as Western Blot, ELISA, and immunohistochemistry. The addition of Azide ensures that the solution remains free of microbial contamination during storage. This solution blocks non-specific binding sites, ensuring that antibodies or proteins of interest bind specifically to their targets, enhancing the accuracy of experimental results. Available in a 500-milliliter bottle, it is the perfect size for routine use in laboratories performing protein analysis or other immunological studies, helping to reduce background noise and improve assay sensitivity.
Alkali Scientific TBS / Tween 20 10X Solution, 500 Milliliters
TBS / Tween 20 10X Solution is a highly effective washing buffer used to minimize background noise during immunodetection procedures such as Western blotting and ELISA. This 500mL solution is ideal for smaller-scale applications or as a concentrate that can be diluted for use in various experimental setups. The Tris component provides pH stability, while Tween 20 aids in reducing non-specific binding during protein-protein interactions. This formulation ensures cleaner results by washing away excess proteins and antibodies that do not bind specifically to the target. TBS-Tween 20 is essential for improving the signal-to-noise ratio in sensitive immunoassays and diagnostic tests.