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Agarose and acrylamide powders, buffers, diluents, markers and stains for protein/nucleic acid electrophoresis; featuring concentration, grade, melting point, capacity to stabilize, control concentration, denature, reduce, and mark target molecules.
Sigmas 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb 1 kb repeats from 3 to 6 kb and 2 kb repeats from 6 to 10 kb. In nucleic acid gel electrophoresis five mul of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide SYBR(R) Green and Nancy-520.
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Sigmas PCR 100 bp Low Marker contains 10 dsDNA fragments from 100 - 1 000 bases evenly spaced in 100 bp repeats. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
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We are committed to bringing you Greener Alternative Products which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.A The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
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Acrylamidenbsp(AA) is a neurotoxin and carcinogen. It was found in potato products like french fries and potato chips and also in several cereal-based products. This highly reactive organic compound has the ability to polymerize to form polyacrylamide.
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Polyacrylamide gels are prepared using acrylamide and bis-acrylamide where bis-acrylamide is a cross-linking agent. On the other hand acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.
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The ready-to-use BLUeye prestained protein ladder has 12 sharp bands at 11 17 20 25 35 48 63 75 100 135 180 and 245 kDa which has more bands than commonly used markers. The BLUeye prestained protein ladder is provided in a gel loading buffer. A green and a red reference band respectively enable easier identification. The BLUeye prestained protein ladder was produced by GeneDirex Taiwan.
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SYPRO Ruby protein gel stain is a ready-to-use ultrasensitive luminescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). This stain designed especially for use in 2-D PAGE has proven to be an excellent choice for 1-D PAGE and isoelectric focusing (IEf) gels as well. SYPRO Ruby protein gel stain attains sensitivity comparable to many silver stain techniques. However unlike silver staining the SYPRO Ruby gel stainuses a simple staining protocol with no possibility of overstainingdelivers a linear quantitation range of over three orders of magnitudeshows less protein-to-protein variabilitystains glycoproteins lipoproteins calcium-binding proteins fibrillar proteins and other difficult-to-stain proteinswill not stain extraneous nucleic acidsdoes not interfere with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry
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Enables cysteine-type endopeptidase activity, protein kinase binding activity, and thiol-dependent deubiquitinase. Involved in G1/S transition of mitotic cell cycle, protein deubiquitination, and regulation of DNA replication. Located in nucleus.
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This gene encodes a member of the SLC25 transporter or mitochondrial carrier family of proteins. Members of this family are encoded by the nuclear genome while their protein products are usually embedded in the inner mitochondrial membrane and exhibit wide-ranging substrate specificity. Although the encoded protein is currently considered an orphan transporter, this protein is related to other carriers known to transport amino acids. This protein may play a role in iron homeostasis.
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The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. The encoded protein is involved in myelopoiesis, and defects in this gene have been associated with several myeloproliferative disorders. Two variants encoding different isoforms have been found for this gene.
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This gene is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. The DNA-binding motifs suggest this protein is involved in the regulation of transcription and chromatin remodeling. Mutations in this gene have been associated with X-linked cognitive disability. Alternative splicing results in multiple transcript variants.
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