Centrifuge Rotors
INTACT GENOMICS INC IG C43(DE3)pLysS Chemically Competent Cells 6x50µl
These chemically competent cells are engineered for high-efficiency transformation and stable expression of toxic or difficult proteins within IPTG-inducible T7 systems. Derived from BL21(DE3), this strain offers improved tolerance to toxic proteins, while the pLysS plasmid works to suppress basal expression, thereby stabilizing challenging constructs.
- T7 expression strain
- Selected for expression of toxic proteins
- Suitable for expression of toxic genes
- Chloramphenicol-resistant (12.5 μg/ml)
- High-efficiency transformation
- Improved tolerance to toxic proteins
- Suppresses basal expression of T7 RNA polymerase
INTACT GENOMICS INC IG C43(DE3) Chemically Competent Cells 6x50µl
Intact Genomics (IG®) C43(DE3) Chemically Competent Cells are optimized for toxic and difficult protein expression using IPTG-inducible T7 promoter vectors. This BL21(DE3) mutant strain was selected from C41(DE3) survivor cells that overexpressed toxic subunit b of E. coli F-ATPase. C43(DE3) has mutations to prevent cell death during recombinant protein expression and effectively expresses toxic proteins from diverse organisms. Performance is patent-validated and supported by numerous publications.
- Optimized for toxic and difficult protein expression.
- Mutant BL21(DE3) derivative with improved protein yield.
- Mutations prevent cell death during recombinant protein expression.
- Effective for expressing toxic proteins from diverse organisms.
- Transformation efficiency ≥ 3.0 x 107 cfu/μg pUC19 DNA.
- Shipped on dry ice.
USA Scientific Inc Dual rotor personal cent
Personal Minicentrifuge, 8 place 1.5/2.0 mL microcentrifuge tube rotor with adapters for both 0.5 mL and 0.2 mL tubes, and PCR strip rotor for up to 6,500 rpm 2,5000 x g