This DNA library preparation kit uses enzyme-based fragmentation for Illumina high-throughput sequencing. It significantly reduces artificial invert chimera reads in FFPE samples, improving SNV and biomarker detection. The kit integrates DNA fragmentation, end repair, and dA-tailing into one step, allowing direct adapter ligation, library amplification, and size selection without additional purification. It can convert 100 pg to 1 μg of template DNA into a library suitable for Illumina platforms.
- Lower false-positive rate for enhanced variant calling.
- Excellent performance with wide template compatibility and high library conversion.
- Integrates DNA fragmentation, end repair, and dA-tailing.
- Direct adapter ligation, library amplification, and size selection.
- Compatible with 100 pg to 1 μg of template DNA.
- Adjustable fragmentation time for desired insert sizes.