PCR Supplies
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Applied Biosystems™ TaqMan™ β-Actin Detection Reagents
TaqMan™ β-Actin Detection Reagents
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ TaqMan™ Rodent GAPDH Control Reagents
TaqMan™ Rodent GAPDH Control Reagents
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ Absolute Q™ Liquid Biopsy dPCR Assay - BRAF 476
Absolute Q™ Liquid Biopsy dPCR assays enable reproducible, specific detection of known somatic mutations.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Control Primer-Probe Set(s) |
| Gene Symbol | 18S |
| Amplicon Size | 187 |
| Concentration | 20X |
| For Use With (Application) | Gene Expression |
| PCR Method | 2-step RT-qPCR |
| Detection Method | Primer-probe |
| Accession Number | X03205.1 |
| Target | 18S rRNA |
| Format | Tube |
| Label or Dye | FAM |
| Species | Eukaryotic, Rat, Mouse, Human |
| Product Line | FAM, TaqMan |
Applied Biosystems™ Protein Thermal Shift™ Dye Kit
This solution significantly reduces the time and cost associated with screening samples relative to currently available methods.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ Absolute Q™ Liquid Biopsy dPCR Assay - NRAS 580
Absolute Q™ Liquid Biopsy dPCR assays enable reproducible, specific detection of known somatic mutations.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ Human B2M (Beta-2-Microglobulin) Endogenous Control (VIC™/MGB probe, primer limited)
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Human B2M (Beta-2-Microglobulin) Endogenous Control (VIC™/MGB probe, primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ DNA Polymerase I/DNase I
An optimized mixture of both enzymes for efficient nick translation of DNA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ T4 DNA Polymerase
DNA polymerase with 3'-exodeoxyribonuclease activity, but not 5'a3' exodeoxyribonuclease activity
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet Ice |
|---|---|
| Polymerase | T4 DNA Polymerase |
| Content And Storage | T4 DNA Polymerase is supplied with a vial of 5X T4 DNA Polymerase buffer [165 mM Tris acetate (pH 7.9), 330 mM sodium acetate, 50 mM magnesium acetate, 5 mM DTT]. Store at -20°C. |
| Description | DNA Polymerase |
| Hot Start | No |
| Exonuclease Activity | 3' - 5' |
Applied Biosystems™ TaqMan™ Gene Expression Master Mix
Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| Purity or Quality Grade | UP (Ultra Pure) |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Product Line | TaqMan |
| Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Multiplex Master Mix
Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | AmpliTaq Fast DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 2 X |
| Product Type | Multiplex Master Mix |
| Sample Type | DNA |
| Reaction Speed | Fast |
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Virus Detection |
| PCR Method | Multiplex qPCR |
| Product Line | TaqMan |
| Detection Method | Primer-probe |
Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | Medium |
| Content And Storage | Store at -15°C to -25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use. |
| Form | Liquid |
| Concentration | 4X |
| For Use With (Application) | Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence |
| PCR Method | 1-step RT-qPCR |
| Multiplex Capability | Up to 5-plex |
| Detection Method | Primer-probe |
| Includes | dUTP |
| Thermostability | 8 hr., Assembled Reaction |
| Polymerase | Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start |
| Format | Single-Tube |
| Passive Reference Dye | ROX (Pre-mixed) |
| Reaction Speed | Fast or Standard |
| Inhibitor Tolerance (Simple) | High |
| Target Specificity | RNA only, Both RNA and DNA |
| Hot Start | Antibody-based |
| Shelf Life | Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt |
| Product Line | TaqPath |
Applied Biosystems™ Fast SYBR™ Green Master Mix
Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Purity or Quality Grade | UP (Ultra Pure) |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Fast |
| Label or Dye | SYBR Green |
| Product Line | SYBR |
Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG
TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet Ice |
|---|---|
| Polymerase | AmpliTaq Gold DNA Polymerase |
| GC-Rich PCR Performance | High |
| Product Type | Universal PCR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
| PCR Method | qPCR |
| Product Line | TaqMan |
| Detection Method | Primer-probe |
Applied Biosystems™ AmpliTaq Gold™ Fast PCR Master Mix
The Applied Biosystems AmpliTaq Gold Fast PCR Master Mix is a premix, hot start chemistry for end-point PCR analysis.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 1X |
| Overhang | 3'-A |
| Concentration | 2X |
| For Use With (Application) | Cloning,Hot-start PCR |
| Exonuclease Activity | 5' - 3' |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Size (Final Product) | 1.5 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Fast PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Starting Material | DNA |