PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.

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586 – 600 of 4,528 results

586

Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)

Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	• RevertAid H Minus Reverse Transcriptase (200 U/μL) • 5X Reaction Buffer Store at –20°C.
Reverse Transcriptase	RevertAid H Minus
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 13 kb
Reaction Format	Separate components
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	42°C to 45°C
Technique	Reverse Transcription
Starting Material	RNA

587

Thermo Scientific Chemicals Taq DNA Polymerase Kit

Pricing & Availability

588

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Applied Biosystems™ TaqMan™ Multiplex Master Mix

Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).

Pricing & Availability
Specifications

Polymerase	AmpliTaq Fast DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Product Type	Multiplex Master Mix
Sample Type	DNA
Reaction Speed	Fast
Concentration	2X
For Use With (Application)	Gene Expression,Virus Detection
PCR Method	Multiplex qPCR
Product Line	TaqMan
Detection Method	Primer-probe

589

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Applied Biosystems™ GeneAmp™ Fast PCR Master Mix (2X), with or without protocol

The GeneAmp Fast PCR Master Mix, with its sensitive, pre-mixed hot-start chemistries, reduces PCR reaction time to as little as 25 minutes when used with the Veriti 96-Well Thermal Cycler.

Pricing & Availability
Specifications

GC-Rich PCR Performance	Medium
Fidelity (vs. Taq)	1X
Overhang	3'-A
Concentration	2X
Exonuclease Activity	5' - 3'
Polymerase	AmpliTaq DNA Polymerase
No. of Reactions	250 Reactions
Format	Tube
Volume	0.1 mL or 0.2 mL
Size (Final Product)	2 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	Fast PCR Master Mix
Reaction Speed	Fast
Hot Start	Built-In Hot Start

590

Thermo Scientific™ Phusion Blood Direct PCR Master Mix

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
GC-Rich PCR Performance	High
Includes Label or Dye	Yes
Overhang	Blunt
Concentration	2X
For Use With (Application)	Genotyping
PCR Method	Direct PCR
Polymerase	Phusion Blood II DNA Polymerase
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Blood
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Starting Material	DNA

591

Thermo Scientific™ DreamTaq™ Buffers (10X)

Thermo Scientific 10X DreamTaq Buffer is specifically optimized for PCR using DreamTaq DNA Polymerase and DreamTaq Hot Start DNA Polymerase.

Pricing & Availability
Specifications

Quantity	4 x 1.25 mL
Concentration	10X
For Use With (Application)	Hot-start PCR

592

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Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Content And Storage	Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Product Type	Universal Master Mix II
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Product Line	TaqMan

593

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Applied Biosystems™ TaqMan™ Array Human PI3K Signaling

Easy to use - Since the primer/probe is supplied in a dry state, Just add the master mix and sample.

Pricing & Availability

594

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Cytiva RAPD Analysis Beads

For performing DNA-profiling experiments using the randomly amplified polymorphic DNA (RAPD) technique

Pricing & Availability
Specifications

Format	Tube
Volume	500 μL
pH	8.3
For Use With (Application)	Standard PCR

595

Invitrogen™ Cells-to-C_T Bulk RT Reagents

Cells-to-C_T Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan™ Gene Expression Cells-to-C_T™ Kit (20X RT enzyme mix and 20X RT buffer).

Pricing & Availability

596

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Applied Biosystems™ TaqMan™ PreAmp Master Mix Kit

TaqMan™ PreAmp Master Mix Kit contains TaqMan™ PreAmp Master Mix and TaqMan™ Gene Expression Master Mix.

Pricing & Availability
Specifications

Content And Storage	Contains 2X TaqMan PreAmp Master Mix (1 mL), TaqMan™ Gene Expression Master Mix (5 mL), and TaqMan™ PreAmp Master Mix Kit Quick Reference Card. Store master mixes at 2-8°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
For Use With (Equipment)	7300 System, 7500 Fast System, 7500 System, 7900HT Fast System
Sample Type	cDNA
Detection Method	Primer-probe

597

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Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)

Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	Medium
Fidelity (vs. Taq)	1X
Format	Tube
Size (Final Product)	5 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	Hot Start PCR Master Mix
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Overhang	3'-A
Concentration	2X
Starting Material	DNA
For Use With (Application)	Hot-start PCR

598

Applied Biosystems™ TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV), 0.1 mL

A simple solution for dengue, chikungunya, and Zika virus RNA detection: just add RNA.

Pricing & Availability

599

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Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System

Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Format	1-step RT-PCR Kit
Reverse Transcriptase	SuperScript IV
Size (Final Product)	Up to 13 kb
Reaction Format	Master Mix
Reagent Type	Reverse Transcription
Hot Start	Built-in Hot Start
Optimal Reaction Temperature	50°C to 65°C
Technique	1-step RT-PCR
Starting Material	RNA
PCR Method	1-step RT-PCR
Multiplex Capability	Yes
Detection Method	Gel Electrophoresis

600

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Applied Biosystems™ GeneAmp™ 10X PCR Gold Buffer & MgCl₂

Applied Biosystems GeneAmp 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold DNA Polymerase, resulting in highly specific, robust PCR amplification.

Pricing & Availability
Specifications

Polymerase	AmpliTaq Gold DNA Polymerase
Format	Kit
Product Type	10X PCR Gold Buffer
Concentration	10X
For Use With (Application)	Standard PCR

PCR Supplies

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Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL) GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Applied Biosystems™ TaqMan™ Multiplex Master Mix GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Invitrogen™ Cells-to-CT Bulk RT Reagents Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)

Applied Biosystems™ TaqMan™ Multiplex Master Mix

Invitrogen™ Cells-to-C_T Bulk RT Reagents