FastAmp Plant Direct RPA kit offers a robust method for amplifying DNA fragments from plant tissues at a single, constant temperature (37°C) using a recombinase (e.g. UvsX), primers, a single-stranded DNA binding protein (SSB), and a strand displacing DNA polymerase. The optimized Isothermal Recombinase Polymerase Amplification (RPA) assay further enhances the utility of the FastAmp Plant Direct RPA kit in genotyping, transgene detection, knockout analysis, and DNA virus diagnostics. The FastAmp Plant Direct RPA Solution is a simple, fast, and reliable step that eliminates the need for DNA extraction and streamlines tissue lysis and genomic DNA prep for RPA in just 3 minutes. Minimizes the need for expensive bead or spin column-based extraction methods. The Kit Includes: FastAmp Plant Direct RPA Solution, IG RPA Enzyme Mixture, IG RPA Reaction Master Mix, Magnesium Acetate (MgOAc), Nuclease-free Water, Micro Pestle, Leaf Puncher, 1.2 mm. Components store at various temperatures.