Hot Start PCR Reagents and Kits
Hot Start PCR Reagents and Kits
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Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase
Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.
Polymerase | DreamTaq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Reaction Format | Separate Components |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Concentration | 5 U/μL |
For Use With (Application) | Hot-start PCR |
Applied Biosystems™ AmpliTaq Gold™ Fast PCR Master Mix
The Applied Biosystems AmpliTaq Gold Fast PCR Master Mix is a premix, hot start chemistry for end-point PCR analysis.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Wet Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Concentration | 2X |
For Use With (Application) | Cloning,Hot-start PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | AmpliTaq Gold DNA Polymerase |
Size (Final Product) | 1.5 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | Fast PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Starting Material | DNA |
Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase
Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
---|---|
Polymerase | Platinum II Taq Hot-Start DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | Hot Start DNA Polymerase |
Reaction Speed | Fast or Standard |
Overhang | 3'-A |
Concentration | 2X |
Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mixes
Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.
Shipping Condition | Dry Ice |
---|---|
Polymerase | DreamTaq DNA Polymerase |
GC-Rich PCR Performance | Low |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Concentration | 2X |
For Use With (Application) | Hot-start PCR |
Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)
Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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GC-Rich PCR Performance | High |
---|---|
Fidelity (vs. Taq) | 1X |
Format | Tube |
Size (Final Product) | 5 kb or less |
Hot Start | Built-In Hot Start |
Concentration | 2X |
For Use With (Application) | Hot-start PCR |
Thermo Scientific™ Phire Hot Start II DNA Polymerase
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.
Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2X |
Purity or Quality Grade | DHPLC |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Starting Material | DNA |
For Use With (Application) | Cloning,Hot-start PCR |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Polymerase | AmpliTaq Gold DNA Polymerase |
---|---|
Fidelity (vs. Taq) | 1X |
Size (Final Product) | 5 kb or less |
Product Type | DNA Polymerase |
Overhang | 3'-A |
Concentration | 5 U/μL |
Exonuclease Activity | 5' - 3' |
Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)
Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Concentration | 2X |
For Use With (Application) | Hot-start PCR |
Polymerase | Platinum Taq DNA Polymerase |
Format | Tube |
Size (Final Product) | 5 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | Hot Start PCR Master Mix |
Reaction Speed | Standard |
Label or Dye | Green |
Hot Start | Built-In Hot Start |
Starting Material | DNA |
Invitrogen™ Platinum™ Taq DNA Polymerase
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Polymerase | Platinum Taq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Format | Tube |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Starting Material | DNA |
For Use With (Application) | Hot-start PCR |
Exonuclease Activity | 5' - 3' |
Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix
AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temp or Wet Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Green Features | Sustainable packaging |
Concentration | 2X |
For Use With (Application) | Cloning,Hot-start PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | AmpliTaq Gold 360 DNA Polymerase |
Format | Tube |
Size (Final Product) | 5 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | PCR Master Mix |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Starting Material | DNA |
Invitrogen™ Platinum™ II Green PCR Buffer (5X)
Invitrogen 5X Platinum II Green PCR Buffer for fast and easy PCR
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Platinum™ Direct PCR Universal Master Mix
Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
---|---|
Polymerase | Platinum II Taq Hot-Start DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Size (Final Product) | 8 kb |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Universal Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Green Features | Less hazardous, less waste |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,Real Time PCR (qPCR) |
Thermo Scientific™ Phire Hot Start II PCR Master Mixes
Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.
Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2X |
Reaction Format | SuperMix or Master Mix |
Product Type | Hot Start PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Cloning,Hot-start PCR |
Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase
AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | AccuPrime GC-Rich DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2X |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
For Use With (Application) | Hot-start PCR |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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GC-Rich PCR Performance | Low |
---|---|
Fidelity (vs. Taq) | 1X |
Product Type | DNA Polymerase With Buffer I |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Concentration | 5 U/μL |
For Use With (Application) | Hot-start PCR |
Exonuclease Activity | 5' - 3' |