Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Invitrogen™ EXPRESS One-Step Superscript™ qRT-PCR Kit, universal
Kit incorporates proven Invitrogen™ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments.
Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
Applied Biosystems™ TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV), 0.1 mL
A simple solution for dengue, chikungunya, and Zika virus RNA detection: just add RNA.
Applied Biosystems™ TrueMark™ Amplification Control I (5 x 107 copies/μL)
The TrueMark Amplification Control I for TrueMark plates contains a linearized multi-target plasmid pool with microbial target sequences used on TrueMark infectious disease research panels.
SureTect™ Salmonella Species PCR Assay
Rapidly detect Salmonella species in food and environmental samples with the Thermo Scientific™ SureTect™ Salmonella Species PCR Assay.
SureTect™ Listeria species PCR Assay
Rapidly detect Listeria species in food and environmental samples with the Thermo Scientific™ SureTect™ Listeria Species PCR Assay.
Applied Biosystems™ TaqMan™ Advanced miRNA cDNA Synthesis Kit
Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription.
Applied Biosystems™ resDNASEQ™ Quantitative CHO DNA Kits
This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ Quantitative CHO DNA System, allows users to detect sub-picogram amounts of residual DNA from CHO cell line in under 5 hours.
Applied Biosystems™ resDNASEQ™ Human Residual DNA Quantitation Kits
The resDNASEQ Human Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from human cell lines used as expression systems for recombinant proteins.
Applied Biosystems™ resDNASEQ™ Quantitative E. coli DNA Kits
This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours.
Thermo Scientific™ SureTect™ Escherichia coli O157:H7 PCR Assay
Simply and accurately detect E. coli O157:H7 on beef meats, dairy, fruits, and vegetables including large test portion sizes with the Thermo Scientific™ SureTect™ E. coli O157:H7 PCR Assay.
Applied Biosystems™ TaqMan™ Vaginal Microbiota Amplification Control
The TaqMan™ Vaginal Microbiota Amplification Control is designed to evaluate the amplification efficacy of the TaqMan Vaginal Microbiota assays. This plasmid serves as a positive control for the TaqMan Vaginal Microbiota Assay collection.
Thermo Scientific™ SureTect™ Salmonella species, Typhimurium and Enteritidis Multiplex PCR Assay
Rapidly detect and differentiate Salmonella species, Salmonella ser. Typhimurium, and Salmonella ser. Enteritidis in a single test with Thermo Scientific™ SureTect™ Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Assay.
Thermo Scientific™ SureTect™ Listeria monocytogenes PCR Assay
Rapidly detect Listeria monocytogenes in food and environmental samples with the Thermo Scientific™ SureTect™ Listeria monocytogenes PCR Assay.
Applied Biosystems™ resDNASEQ™ Quantitative HEK293 DNA Kits
The resDNASEQ Quantitative HEK293 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host-cell DNA from HEK293 cell lines.