Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
New England Biolabs, Inc. LunaScript RT SuperMix Kit – 100 reactions
LunaScript RT SuperMix Kit features an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It includes the thermostable Luna Reverse Transcriptase, which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is also included to protect template RNA from degradation. The LunaScript RT SuperMix contains random hexamer and poly-dT primers, allowing even coverage across the length of the RNA targets. In addition, the LunaScript RT SuperMix Kit contains a blue dye, providing a visual indicator that can be followed throughout the two-step RT-qPCR process. The LunaScript RT SuperMix offers robust, linear, and sensitive detection using total RNA inputs as high as 1 µg and as low as single copies of RNA.
Norgen Biotek Corp 2X One-Step Rt-Pcr Mas 100Rxns
Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add the template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA templates with high yields of PCR products.Taq Source: An E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1RT Source: An E. coli strain with a cloned reverse transcriptase gene from M-MuLV.
New England Biolabs, Inc. OneTaq RT-PCR Kit – 30 reactions
OneTaq RT-PCR Kit combines two powerful mixes, M-MuLV Enzyme Mix and OneTaq Hot Start 2X Master Mix with Standard Buffer for 2-step RT-PCR applications. The two mixes require minimal handling during reaction setup and yet offer consistent and robust RT-PCR reactions. The first strand cDNA synthesis is achieved by using two optimized mixes, M-MuLV Enzyme Mix and M-MuLV Reaction Mix. M-MuLV Enzyme Mix combines M-MuLV Reverse Transcriptase and murine RNase Inhibitor while M-MuLV Reaction Mix contains dNTPs and an optimized buffer. The kit also contains two optimized primers for reverse transcription and nuclease-free water. An anchored oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail. The optimized Random Primer Mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The amplification step features a OneTaq Hot Start DNA Polymerase in a master mix format.
ATCC Acholeplasma laidlawii
Acholeplasma laidlawii strain PG8 [NCTC 10116, PG8; A] is a whole-genome sequenced bacterial type strain isolated from sewage. This strain is recommended as a test microorganism for USP <63>.
New England Biolabs, Inc. Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit - 96 rxn
The Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit is optimized for real-time qualitative detection of SARS-CoV-2 nucleic acid using hydrolysis probes. It features the Luna Probe One-Step RT-qPCR 4X Mix with UDG, an optimized SARS-CoV-2 Primer/Probe mix containing primers and probes specific to two regions of the SARS-CoV-2 virus N-gene, and a positive control template. The probes have been modified to contain different fluorophores (N1, HEX; N2, FAM) to enable simultaneous observation on two different channels of a real-time instrument. To ensure the integrity of the input material and absence of inhibition, an internal control (IC) primer and probe set, designed to amplify the human RNaseP gene, is also provided in the primer mix. The reverse primer of this target has been modified from the CDC design to target an exon/exon boundary to reduce background amplification from possible contaminating genomic DNA. Amplification of the IC is observed in the Cy5 channel.
Biochain Institute Inc dATP, 100 uL (100 mM)/PK
BioChain's dNTP reagent combines the highest quality with the most competitive price on the market. The dNTPs are purified with preparative HPLC to achieve at least 99.5% purity.
New England Biolabs, Inc. Luna Probe One-Step RT-qPCR 4X Mix with UDG (No ROX) 500 reactions
The Luna Probe One-Step RT-qPCR Mix with UDG (No ROX) is supplied at a 4X concentration and enables higher amounts of sample input which is relevant for applications where RNA present in low abundance is of interest such as pathogen detection. The mix consolidates the components for one-step RT-qPCR into a single tube including Luna WarmStart RT Hot Start Taq DNA Polymerase dNTPs and Murine RNase Inhibitor in an optimized buffer. Combining Hot Start Taq DNA Polymerase with a novel WarmStart-activated reverse transcriptase allows for dual control of enzyme activity via reversible aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling providing added security for setting up reactions at room temperature. This formulation contains no reference dye and is compatible with any instrument that does not require ROX (if ROX normalization is needed ROX can be added.)
Alkali Scientific Radiant™ Probe Lo-ROX qPCR Kit, 2,000 Reactions, (20 x 1mL)
Alkali Scientific offers Radiant™ Probe Lo-ROX qPCR Kits, a high-performance portfolio of 2X ready-to-use real-time PCR reagents designed for best-in-class quantitative PCR. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and exceptional speed (rapid extension rates). The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to dramatic improvements in PCR sensitivity in low-copy assays and PCR conditions conducive to ultra-fast amplification. Furthermore, Radiant™ Probe Lo-ROX qPCR Kits are optimized for advanced multiplexing capability, delivering the same high efficiency, early Ct, and reaction speed even in complex multiplex assays.
Integrated DNA Technologies Inc PrimeTime Gene Expression Master Mix, High PCR Efficiency >90%, Compatible With Various qPCR Systems, 5 mL
The PrimeTime Gene Expression Master Mix is a 2X concentrated solution that enhances accuracy and efficiency in qPCR. It guarantees PCR efficiencies over 90% and R² values above 0.99, making it reliable for gene expression analysis. The mix features exceptional stability at room temperature and high temperatures, reducing shipping costs and environmental impact. Its broad compatibility with various primers and probes differentiates it in the market, perfect for both routine and high-throughput applications.
New England Biolabs, Inc. Luna Cell Ready Probe One-Step RT-qPCR Kit – 100 reactions
The Luna Cell Ready Probe One-Step RT-qPCR Kit provides all the necessary components for direct, probe-based RNA detection and quantitation, bypassing the need for RNA extraction and purification. The Luna Cell Ready Probe One-Step RT-qPCR Kit is composed of: 1) the Luna Cell Ready Lysis Module and 2) the Luna Universal Probe One-Step RT-qPCR Kit. Coordinating the actions of DNase I and the Luna Cell Ready Protease, the Luna Cell Ready Lysis Module offers a simple alternative workflow resulting in effective cell lysis, RNA release, and genomic DNA removal simultaneously in a 15-minute protocol. The Lysis Module includes a unique Luna Cell Ready RNA Protection Reagent that maintains RNA integrity during cell lysis. The lysis capacity spans 10 -100,000 cells in a 50 µl lysis reaction. Up to 2 µl of lysate (equivalent to RNA from 0.2 - 4,000 cells) can be transferred into 20 µl downstream RT-qPCR reactions.
Alkali Scientific Radiant™ HRM Mix, High resolution melt - SNP detection, 500 Reactions, (5 x 1mL)
Radiant™HRM Mix produces ultra-sensitive melt profiles allowing accurate discrimination subtle sequence variations including class I to class IV mutations and CpG methylation differences. Fast and accurate detection of subtle sequence differences Ultra-sensitive fluorescence profiles for clear characterisation of samples Third generation saturating dye SyGreen 2 Antibody-mediated hot start technology for improved sensitivity Compatible with all real-time instruments with HRM capability
AMPLYUS LLC miniPCR bio miniPCR Sleep Lab™: Lark or Owl?
Participate in an authentic investigation on sleep genetics. Are you a lark or an owl? Link genotype to phenotype by exploring the genetics behind individual sleep and wake preferences.