Real Time PCR Reagents and Kits
New England Biolabs, Inc. Luna® Universal One-Step RT-qPCR Kit – 500 reactions
In the Luna One-Step RT-qPCR Kit, Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended.
- Non-interfering, visible tracking dye helps to eliminate pipetting errors
New England Biolabs, Inc. Luna® Universal Probe One-Step RT-qPCR Kit – 2500 reactions
In the Luna Universal One-Step Probe RT-qPCR Kit, Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended.
- Non-interfering, visible tracking dye helps to eliminate pipetting errors
Andwin Scientific QSCRIPT 1STEP TOUGHMX 500X20UL
The qScript XLT One-Step RT-qPCR ToughMix by Quanta Biosciences is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates with the use of hybridization probe detection chemistries like the TaqMan 5-hydrolysis probes on real-time PCR systems that do not need a passive reference dye. This product contains: reagent description, qScript XLT One-Step RT-qPCR ToughMix (2X), 2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride, qScript XLT reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase, AccuVue blue qPCR dye, and stabilizers.
