Cell Culture Reagents, Antibiotics and Supplements
Agilent Technologies™ XF 100 mM Pyruvate Solution, 50 mL
XF 100 mM Pyruvate Solution is specifically formulated for Agilent™ Seahorse XF assays to achieve optimal results.
Agilent Technologies™ XF 1 M Glucose Solution, 50 mL
XF 1 M Glucose Solution specifically formulated for Agilent™ Seahorse XF assays to achieve optimal results.
Agilent Technologies™ XF 200 mM Glutamine Solution, 50 mL
XF 200 mM Glutamine Solution is specifically formulated for Agilent™ Seahorse XF assays to achieve optimal results.
Gibco™ Soybean Trypsin Inhibitor, powder
Trypsin inhibited at a molar ratio of 1:1 and chymotrypsin and plasmin to lesser extent
Gibco™ Collagenase, Type II, powder
Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen
STEMCELL Technologies Collagenase/Hyaluronidase, Size: 10 mL
10X Collagenase/Hyaluronidase in Dulbecco’s Modified Eagle’s Medium (DMEM) for the enzymatic dissociation of human mammary tissue.
Sigma Aldrich Fine Chemicals Biosciences Penicillin-Streptomycin, 100 mL
Supplement in Dulbecco′s modified Eagle′s medium (DMEM) to maintain the Huh7 liver hepatoma cell line. Supplement in Roswell park memorial institute-1640 (RPMI) medium to maintain mouse lymphoma YAC-1 cells. A component in minimum essential medium Eagle to treat individual mouse spleens for the preparation of splenocytes.
STEMCELL Technologies MyoCult Expansion 10X Supplement (Mouse), Size: 10 mL
MyoCult Expansion 10X Supplement (Mouse) when combined with DMEM/F-12 to prepare MyoCult Expansion Medium is for the culture of mouse skeletal muscle progenitor cells (satellite cells). MyoCult Expansion Medium has been optimized for the expansion of mouse skeletal muscle satellite cells isolated by fluorescence-activated cell sorting (FACS) as well as for culturing single isolated myofibers grown in suspension. Satellite cells cultured using MyoCult Expansion Medium can be differentiated into multinucleated myotubes
STEMCELL Technologies ClonaCell™-HY PEG, Size: 1.5 mL
ClonaCell™-HY PEG is suitable for inducing cellular fusion of myeloma cells with lymphocytes to produce hybridomas. This reagent has been verified for use during mouse and rat hybridoma development and reportedly is compatible for production, cloning, and expansion of hybridomas using lymphocytes from a variety of host animals including human, mouse, rat, and hamster. ClonaCell™-HY PEG is supplied as a 50% (w/v) solution of PEG 3350 in Dulbecco's Modified Eagle's Medium (DMEM) with gentamicin and phenol red. The solution has been pre-tested and qualified for cell fusion.